Question 1

In Southern blotting,DNA molecules are immobilized on

Accepted Answer

A)  silicon chips. 
B)  nitrocellulose membranes. 
C)  agarose. 
D)  gold beads. 
E)  paper. 
A)  silicon chips. 
B)  nitrocellulose membranes. 
C)  agarose. 
D)  gold beads. 
E)  paper.

Question 2

Genetic (screening/sequencing/cloning)can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Accepted Answer

The answer of Genetic (screening/sequencing/cloning)can be used to detect mutant...

Question 3

Injecting DNA into cells can be accomplished using

Accepted Answer

A)  nitrocellulose membranes. 
B)  silicon chips and nucleic acids. 
C)  compressed air and gold beads. 
D)  micropipettes. 
E)  micropipettes, or compressed air and gold beads. 
A)  nitrocellulose membranes. 
B)  silicon chips and nucleic acids. 
C)  compressed air and gold beads. 
D)  micropipettes. 
E)  micropipettes, or compressed air and gold beads.

Question 4

Synthetic nucleic acids are useful as

Accepted Answer

A)  DNA probes. 
B)  primers for PCR. 
C)  antisense RNAs. 
D)  DNA probes and antisense RNAs. 
E)  DNA probes, primers, and antisense RNAs. 
A)  DNA probes. 
B)  primers for PCR. 
C)  antisense RNAs. 
D)  DNA probes and antisense RNAs. 
E)  DNA probes, primers, and antisense RNAs.

Question 5

DNA encoding a normal gene is inserted into cells from a patient with a defective form of the gene.Recombinant cells are identified and isolated,and returned to the patient's body.This is an example of

Accepted Answer

A)  gene therapy. 
B)  genomics. 
C)  genetic mapping. 
D)  genotyping. 
E)  biotechnology. 
A)  gene therapy. 
B)  genomics. 
C)  genetic mapping. 
D)  genotyping. 
E)  biotechnology.

Question 6

The technique called (northern/Southern/western)blotting is useful for using probes to identify specific DNA fragments that have been separated by electrophoresis.

Accepted Answer

The answer of The technique called (northern/Southern/western)blotting is useful for...

Question 7

In the 20th century,scientists harnessed the natural metabolic reactions of bacteria to make ________ for the first time in an industrial setting.

Accepted Answer

A)  soy sauce 
B)  acetone 
C)  wine 
D)  cheese 
E)  alcohol 
A)  soy sauce 
B)  acetone 
C)  wine 
D)  cheese 
E)  alcohol

Question 8

Which method of inserting DNA into cells is illustrated in Figure 8.1?

Accepted Answer

A)  protoplast fusion 
B)  injection 
C)  electroporation 
D)  a gene gun 
E)  transduction 
A)  protoplast fusion 
B)  injection 
C)  electroporation 
D)  a gene gun 
E)  transduction

Question 9

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

A) True 
 B)False

Question 10

The procedure used to determine whether a person has a heritable disease is known as

Accepted Answer

A)  DNA sequencing. 
B)  microarray analysis. 
C)  genetic screening. 
D)  northern analysis. 
E)  xenotransplantation. 
A)  DNA sequencing. 
B)  microarray analysis. 
C)  genetic screening. 
D)  northern analysis. 
E)  xenotransplantation.

Question 11

A collection of bacterial clones each of which contains a portion of the gene sequences of an organism is known as a microbiome of that organism's genome.

Accepted Answer

A) True 
 B)False

Question 12

Restriction (enzymes/proteases/polymerases),first isolated from bacterial cells,cut DNA molecules at specific sites.

Accepted Answer

The answer of Restriction (enzymes/proteases/polymerases),first isolated from bacterial cells,cut DNA...

Question 13

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

Accepted Answer

The answer of A set of clones representing the entire...

Question 14

Which of the following is NOT a goal of recombinant DNA technology?

Accepted Answer

A)  combining genetic material from more than one organism to produce new useful organisms 
B)  making it possible to clone humans 
C)  eliminating undesirable traits from livestock or crops 
D)  being able to remove or correct damaging traits in humans 
E)  creating organisms capable of producing useful products 
A)  combining genetic material from more than one organism to produce new useful organisms 
B)  making it possible to clone humans 
C)  eliminating undesirable traits from livestock or crops 
D)  being able to remove or correct damaging traits in humans 
E)  creating organisms capable of producing useful products

Question 15

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells.Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?

Accepted Answer

A)  Sequence the DNA of the plasmids from each isolate. 
B)  Use a microarray to detect a transcript of the gene. 
C)  Isolate clones that still have the vector's genetic marker. 
D)  Assay for activity of the gene product. 
E)  Use a northern blot to detect the mRNA of the gene. 
A)  Sequence the DNA of the plasmids from each isolate. 
B)  Use a microarray to detect a transcript of the gene. 
C)  Isolate clones that still have the vector's genetic marker. 
D)  Assay for activity of the gene product. 
E)  Use a northern blot to detect the mRNA of the gene.

Exam 8: Recombinant DNA Technology

In Southern blotting,DNA molecules are immobilized on

Genetic (screening/sequencing/cloning)can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Injecting DNA into cells can be accomplished using

Synthetic nucleic acids are useful as

DNA encoding a normal gene is inserted into cells from a patient with a defective form of the gene.Recombinant cells are identified and isolated,and returned to the patient's body.This is an example of

The technique called (northern/Southern/western)blotting is useful for using probes to identify specific DNA fragments that have been separated by electrophoresis.

In the 20th century,scientists harnessed the natural metabolic reactions of bacteria to make ________ for the first time in an industrial setting.

Which method of inserting DNA into cells is illustrated in Figure 8.1?

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

The procedure used to determine whether a person has a heritable disease is known as

A collection of bacterial clones each of which contains a portion of the gene sequences of an organism is known as a microbiome of that organism's genome.

Restriction (enzymes/proteases/polymerases),first isolated from bacterial cells,cut DNA molecules at specific sites.

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

Which of the following is NOT a goal of recombinant DNA technology?

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells.Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest?

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Pathogenic Gram Positive Bacteria

Pathogenic Gram-Negative Cocci and Bacilli

Rickettsias, Chlamydias, Spirochetes, and Vibrios

Pathogenic Fungi

Parasitic Protozoa, Helminths, and Arthropod Vectors

Pathogenic DNA Viruses

Pathogenic RNA Viruses

Applied and Environmental Microbiology

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