Exam 11: Regulation of Gene Expression in Bacteria and Their Viruses

DNA-dependent RNA polymerase binds to the:

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -mutation that reverses the effect of another mutation at a different site

The lac repressor protein controls expression of the lac operon by binding to the:

In the bacteriophage $\lambda$ ,the N gene encodes:

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -phenotype of I⁺ O⁺ Z^- / I⁺ O^C Z⁺

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -phenotype with respect to $\beta$ -galactosidase synthesis for the partial diploid I⁺ O⁺ Z⁺ / I^- O^C Z^-

The lac operon is the classic "inducible system" for gene control,while the trp operon is the classic "repressible system." Arguably,the key difference between these control systems involves the manner in which the repressor proteins control transcription.Compare and contrast the LacI repressor protein with the TrpR repressor protein.

A partial diploid of genotype I^S P⁺ O⁺ Z⁺ / I⁺ P⁺ O⁺ Z^- will show:

The trp operon is controlled by:

Predict whether $\beta$ -galactosidase will be produced by the following E.coli strains under the conditions noted.The diploid genotypes represent F' (lac)strains.Use + to indicate that the enzyme is synthesized at greater than basal levels,and 0 to indicate that the enzyme is not synthesized. $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ $\quad$ Lactose absent Lactose present a) $I ^ { + } P ^ { + } O ^ { + } Z ^ { + }$ b) $\mathrm { I } ^ { - } \mathrm { P } ^ { + } \mathrm { O } ^ { + } \mathrm { Z } ^ { + }$ c) $\mathrm { I } ^ { + } \mathrm { P } ^ { + } \mathrm { O } ^ { \mathrm { C } } \mathrm { Z } ^ { + }$ d) $I ^ { - } P ^ { + } O ^ { \mathrm { C } } Z ^ { + }$ e) $I ^ - P ^ { + } O ^ { \mathrm { C } } Z ^ { - }$ f) $\mathrm { I } ^ { + } \mathrm { P } ^ { + } \mathrm { O } ^ { + } \mathrm { Z } ^ { - }$ ?) $\mathrm { I } ^ { + } \mathrm { P } ^ { + } \mathrm { O } ^ { \mathrm { C } } \mathrm { Z } ^ { - } / \mathrm { I } ^ { + } \mathrm { P } ^ { + } \mathrm { O } ^ { + } \mathrm { Z } ^ { + }$ h) $I ^ { + } P ^ { + } O ^ { + } Z ^ { - } / I ^ { + } P ^ { + } O ^ { \mathrm { C } } Z ^ { + }$ i) $I ^ { + } P ^ { + } O ^ { + } Z ^ { - } / I ^ { - } P ^ { + } O ^ { + } Z ^ { + }$ j) $\mathrm { I } ^ { + } \mathrm { P } ^ { + } \mathrm { O } ^ { + } \mathrm { Z } ^ { + } / \mathrm { I } ^ { - } \mathrm { P } ^ { + } \mathrm { O } ^ { + } \mathrm { Z } ^ { - }$ k) $I ^ { + } P ^ { + } O ^ { \mathrm { C } } Z ^ { - } / I^- P ^ { + } O ^ { + } Z ^ { + }$ 1) $I ^ { + } P ^ { + } O ^ { + } Z ^ { - } / I ^ { - } P ^ { + } O ^ { \mathrm { C } } Z ^ { + }$ m) $I ^ { - } P ^ { + } O ^ { + } Z ^ { - } / I^- P ^ { + } O ^ { C } Z ^ { + }$ 1) $I ^ { - } P ^ { + } O ^ { \mathrm { C } } Z ^ { - } / I^- P ^ { + } O ^ { + } Z ^ { + }$

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -phenotype with respect to the synthesis of tryptophan enzymes in the trp operon trp R⁺ O^C S⁺ (trpR codes for repressor;S = structural gene)

The product of the regulator gene of the lac operon is

The lac repressor (LacI)binds to:

In the bacteriophage $\lambda$ ,the mutants cI,cII,and cIII form:

Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l), followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -During lytic cycle of lambda phage,genes concerned with DNA replication are transcribed before genes specifying head proteins,which are transcribed before tail proteins.