Deck 8: Control of Microorganisms in the Environment

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Question
An agent that prevents the growth of bacteria without causing irreversible damage to the bacteria is referred to as __________.
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Question
When antiseptics and disinfectants are compared,antiseptics are generally

A)less toxic.
B)more toxic.
C)equally as toxic.
D)unpredictable in toxicity.
Question
Sterilization involves __________ all viable microorganisms.

A)killing
B)removing
C)killing OR removing
D)metabolically inactivating
Question
A(n)__________ is a chemical that can be used to sterilize materials.

A)oxidant
B)disinfectant
C)antiseptic
D)sterilant
Question
The rate of killing by an antimicrobial agent may slow when the microbial population has been greatly reduced because the remaining population may have a high proportion of resistant organisms.
Question
Which of the following influence(s)the efficiency of an antimicrobial agent?

A)Concentration of the agent.
B)Duration of exposure.
C)Temperature.
D)All of the choices.
Question
The prevention of infection caused by microorganisms is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
Question
An agent that kills bacteria is referred to as __________.
Question
The substantial reduction of the total population of microorganisms on inanimate objects and the destruction of potential pathogens is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
Question
Larger populations generally are killed as rapidly as smaller populations.
Question
Microorganisms show differential sensitivity to antimicrobial agents.
Question
Which will require a longer time to kill?

A)A larger population of microorganisms.
B)A smaller population of microorganisms.
C)Killing will be equally as rapid in a large or a small microbial population.
D)There is no way to predict which will require a longer kill time.
Question
Which of the following inhibits bacterial growth but does not kill bacteria

A)Lysozyme.
B)Bactericidal agent.
C)Bacteriostatic agent.
D)Antiseptic agent.
Question
All germicides are capable of killing all pathogenic organisms and endospores.
Question
Which of the following represents the best definition for microbial death?

A)The organism will not grow on minimal medium.
B)The organism will not grow on a medium that normally supports its growth.
C)The organism no longer retains its original shape and structures.
D)None of these adequately describe microbial death.
Question
The reduction of the microbial population to levels that are considered safe by public health standards is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
Question
An agent that specifically kills fungi but not other kinds of microorganisms is also known as a

A)fungistatic agent.
B)mycostatic agent.
C)fungicidal agent.
D)germicidal agent.
Question
Which of the following environmental factors generally do(es)not have an impact on the efficiency of an antimicrobial agent?

A)PH.
B)Presence or absence of a biofilm.
C)Concentration of organic matter.
D)Refractive index.
Question
The time required to kill 90% of the microorganisms or spores in a sample at a specified temperature is the

A)thermal death time (TDT).
B)thermal death point (TDP).
C)decimal reduction time (D value).
D)z value.
Question
The destruction or removal of all viable organisms is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
Question
Which of the following is not a method of heat sterilization?

A)Autoclaving.
B)Incineration.
C)Pasteurization.
D)Heating in a dry air oven.
Question
Which of the following lacks sufficient penetrating power for bulk sterilization?

A)Ultraviolet (UV)radiation at 260 nm.
B)X rays.
C)Gamma radiation.
D)Alpha and beta particles.
Question
If one left a "Pasteurized" flask of broth for a long time at room temperature,it would stay sterile forever,at least in principle.
Question
Which of the following is MOST effective against resistant endospores?

A)autoclaving
B)boiling
C)pasteurization
D)All of these are equally effective against resistant endospores.
Question
Because filtration removes rather than destroys microorganisms,it does not truly sterilize the materials passing through the filter.
Question
Gamma radiation

A)is a type of nonionizing radiation.
B)has poor penetrating power.
C)is used to sterilize some food products.
D)has a longer wavelength than UV radiation.
Question
The type of filter used in a laminar flow biological safety cabinet is called a(n)__________ filter.
Question
Ultraviolet radiation is an effective means of sterilizing surfaces.
Question
Which of the following is true about membrane filters?

A)It can be used only to remove organisms from liquids.
B)It does not truly sterilize because it removes rather than kills microorganisms.
C)It can be used only to remove organisms from liquids and it does not truly sterilize because it removes rather than kills microorganisms.
D)It can't be depended on to remove all viruses from liquids.
Question
The first product to be commercially treated by pasteurization was

A)milk
B)wine
C)beer
D)cheese
Question
Dry heat methods usually require lower temperatures and shorter exposure times than moist heat methods to achieve the same degree of killing because of the drying effects of this form of heat.
Question
Pasteurization is used to

A)Kill any pathogens present.
B)Retard spoilage.
C)Sterilize beverages.
D)Kill any pathogens present and retard spoilage.
Question
Heavy metals may inactivate proteins by reacting with their sulfhydryl groups.
Question
Which kind of sterilization procedure takes advantage of the size of microorganisms?

A)autoclaving
B)open flame
C)use of chemicals
D)filtration
Question
When comparing dry heat and moist heat sterilization,dry heat is

A)faster.
B)slower.
C)equally as fast.
D)sometimes faster but sometimes slower.
Question
Moist heat sterilization at 100 °\degree C kills all of the following except

A)bacterial endospores.
B)fungal spores.
C)viruses.
D)bacterial endospores and fungal spores.
Question
Joseph Lister

A)pioneered the use of gamma rays for sterilizing food.
B)demonstrated that microorganisms are carried on dust particles in the air.
C)revolutionized surgery by introducing phenol as a disinfectant.
D)designed and built the first autoclave.
Question
Which of these is an agent that is used to sterilize the plastic tubing of heart-lung machines?

A)phenol
B)mercaptoethanol
C)ethylene oxide
D)70% ethanol
Question
Which of the following is a function of pasteurization?

A)killing pathogenic microorganisms
B)reducing the total microbial population
C)increasing the shelf life of the product
D)all of the choices
Question
Moist heat sterilizes by

A)causing the formation of thymine dimers.
B)denaturing proteins.
C)causing production of singlet oxygen.
D)all of the choices
Question
The higher the phenol coefficient value,the _______ effective the disinfectant under the test conditions.
Question
Amphipathic organic molecules that serve as disinfectants by disrupting membranes and denaturing proteins are called

A)sterilants.
B)oxidants.
C)detergents.
D)soaps.
Question
Antiseptics are generally regulated by the

A)Food and Drug Administration.
B)Environmental Protection Agency.
C)The National Institutes of Health.
D)Centers for Disease Control and Prevention.
Question
Although heavy metals are no longer widely used as germicides,__________ is an effective algicide in lakes and swimming pools.

A)lead acetate
B)copper sulfate
C)silver nitrate
D)mercuric chloride
Question
Bacteriophage therapy was developed after antibiotics were first discovered and used for treating bacterial infections.
Question
The disinfectant of choice for municipal water supplies is __________.

A)fluorine
B)chlorine
C)either fluorine or chlorine
D)ultraviolet light
Question
The disinfectant action of phenol and phenolic derivatives mainly is due to

A)its inherent detergent action.
B)membrane damage and protein denaturation.
C)oxidation of disulfide bonds in proteins.
D)extraction of lipids from membranes.
E)damage to nucleic acids and proteins caused by free radicals.
Question
The disinfecting properties of Lysol,a common household disinfectant,depend upon the presence of __________.
Question
The disinfectant screening method that is known as the ________ _________ test,which is used to measure the potency of a disinfectant.
Question
Iodine can be complexed with an organic carrier to form water-soluble,stable complexes called __________,which release iodine slowly and eliminate skin burns and irritation associated with iodine use.

A)iodides
B)tinctures
C)iodophors
D)iodochromes
Question
Heavy metals are effective antimicrobial agents but are not widely used because of their high toxicity to humans.
Question
The two most important alcohol germicides are __________ and __________.

A)ethanol; methanol
B)ethanol; isopropanol
C)methanol; butanol
D)methanol; isopropanol
Question
Alcohols are widely used as antiseptics and disinfectants because they are effective against endospores as well as vegetative cells.
Question
Which of the following is (are)a sterilizing gas(es)that can be used on heat-sensitive materials?

A)ethylene oxide
B)glutaraldehyde
C)chlorine gas
D)isopropanol
Question
Disinfectants are generally regulated by the

A)Food and Drug Administration.
B)Environmental Protection Agency.
C)The National Institutes of Health.
D)Centers for Disease Control and Prevention.
Question
Betapropiolactone is not as useful as ethylene oxide as a sterilizing agent because it does not penetrate materials as readily as ethylene oxide.
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Deck 8: Control of Microorganisms in the Environment
1
An agent that prevents the growth of bacteria without causing irreversible damage to the bacteria is referred to as __________.
bacteriostatic
2
When antiseptics and disinfectants are compared,antiseptics are generally

A)less toxic.
B)more toxic.
C)equally as toxic.
D)unpredictable in toxicity.
A
3
Sterilization involves __________ all viable microorganisms.

A)killing
B)removing
C)killing OR removing
D)metabolically inactivating
C
4
A(n)__________ is a chemical that can be used to sterilize materials.

A)oxidant
B)disinfectant
C)antiseptic
D)sterilant
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5
The rate of killing by an antimicrobial agent may slow when the microbial population has been greatly reduced because the remaining population may have a high proportion of resistant organisms.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
6
Which of the following influence(s)the efficiency of an antimicrobial agent?

A)Concentration of the agent.
B)Duration of exposure.
C)Temperature.
D)All of the choices.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
7
The prevention of infection caused by microorganisms is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
8
An agent that kills bacteria is referred to as __________.
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k this deck
9
The substantial reduction of the total population of microorganisms on inanimate objects and the destruction of potential pathogens is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
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k this deck
10
Larger populations generally are killed as rapidly as smaller populations.
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k this deck
11
Microorganisms show differential sensitivity to antimicrobial agents.
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12
Which will require a longer time to kill?

A)A larger population of microorganisms.
B)A smaller population of microorganisms.
C)Killing will be equally as rapid in a large or a small microbial population.
D)There is no way to predict which will require a longer kill time.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
13
Which of the following inhibits bacterial growth but does not kill bacteria

A)Lysozyme.
B)Bactericidal agent.
C)Bacteriostatic agent.
D)Antiseptic agent.
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k this deck
14
All germicides are capable of killing all pathogenic organisms and endospores.
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15
Which of the following represents the best definition for microbial death?

A)The organism will not grow on minimal medium.
B)The organism will not grow on a medium that normally supports its growth.
C)The organism no longer retains its original shape and structures.
D)None of these adequately describe microbial death.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
16
The reduction of the microbial population to levels that are considered safe by public health standards is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
17
An agent that specifically kills fungi but not other kinds of microorganisms is also known as a

A)fungistatic agent.
B)mycostatic agent.
C)fungicidal agent.
D)germicidal agent.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
18
Which of the following environmental factors generally do(es)not have an impact on the efficiency of an antimicrobial agent?

A)PH.
B)Presence or absence of a biofilm.
C)Concentration of organic matter.
D)Refractive index.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
19
The time required to kill 90% of the microorganisms or spores in a sample at a specified temperature is the

A)thermal death time (TDT).
B)thermal death point (TDP).
C)decimal reduction time (D value).
D)z value.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
20
The destruction or removal of all viable organisms is called

A)disinfection.
B)antisepsis.
C)sterilization.
D)sanitization.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
21
Which of the following is not a method of heat sterilization?

A)Autoclaving.
B)Incineration.
C)Pasteurization.
D)Heating in a dry air oven.
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Unlock Deck
k this deck
22
Which of the following lacks sufficient penetrating power for bulk sterilization?

A)Ultraviolet (UV)radiation at 260 nm.
B)X rays.
C)Gamma radiation.
D)Alpha and beta particles.
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Unlock Deck
k this deck
23
If one left a "Pasteurized" flask of broth for a long time at room temperature,it would stay sterile forever,at least in principle.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
24
Which of the following is MOST effective against resistant endospores?

A)autoclaving
B)boiling
C)pasteurization
D)All of these are equally effective against resistant endospores.
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k this deck
25
Because filtration removes rather than destroys microorganisms,it does not truly sterilize the materials passing through the filter.
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Unlock Deck
k this deck
26
Gamma radiation

A)is a type of nonionizing radiation.
B)has poor penetrating power.
C)is used to sterilize some food products.
D)has a longer wavelength than UV radiation.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
27
The type of filter used in a laminar flow biological safety cabinet is called a(n)__________ filter.
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k this deck
28
Ultraviolet radiation is an effective means of sterilizing surfaces.
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Unlock Deck
k this deck
29
Which of the following is true about membrane filters?

A)It can be used only to remove organisms from liquids.
B)It does not truly sterilize because it removes rather than kills microorganisms.
C)It can be used only to remove organisms from liquids and it does not truly sterilize because it removes rather than kills microorganisms.
D)It can't be depended on to remove all viruses from liquids.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
30
The first product to be commercially treated by pasteurization was

A)milk
B)wine
C)beer
D)cheese
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Unlock Deck
k this deck
31
Dry heat methods usually require lower temperatures and shorter exposure times than moist heat methods to achieve the same degree of killing because of the drying effects of this form of heat.
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Unlock Deck
k this deck
32
Pasteurization is used to

A)Kill any pathogens present.
B)Retard spoilage.
C)Sterilize beverages.
D)Kill any pathogens present and retard spoilage.
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Unlock Deck
k this deck
33
Heavy metals may inactivate proteins by reacting with their sulfhydryl groups.
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Unlock Deck
k this deck
34
Which kind of sterilization procedure takes advantage of the size of microorganisms?

A)autoclaving
B)open flame
C)use of chemicals
D)filtration
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
35
When comparing dry heat and moist heat sterilization,dry heat is

A)faster.
B)slower.
C)equally as fast.
D)sometimes faster but sometimes slower.
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Unlock Deck
k this deck
36
Moist heat sterilization at 100 °\degree C kills all of the following except

A)bacterial endospores.
B)fungal spores.
C)viruses.
D)bacterial endospores and fungal spores.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
37
Joseph Lister

A)pioneered the use of gamma rays for sterilizing food.
B)demonstrated that microorganisms are carried on dust particles in the air.
C)revolutionized surgery by introducing phenol as a disinfectant.
D)designed and built the first autoclave.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
38
Which of these is an agent that is used to sterilize the plastic tubing of heart-lung machines?

A)phenol
B)mercaptoethanol
C)ethylene oxide
D)70% ethanol
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
39
Which of the following is a function of pasteurization?

A)killing pathogenic microorganisms
B)reducing the total microbial population
C)increasing the shelf life of the product
D)all of the choices
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Unlock Deck
k this deck
40
Moist heat sterilizes by

A)causing the formation of thymine dimers.
B)denaturing proteins.
C)causing production of singlet oxygen.
D)all of the choices
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
41
The higher the phenol coefficient value,the _______ effective the disinfectant under the test conditions.
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k this deck
42
Amphipathic organic molecules that serve as disinfectants by disrupting membranes and denaturing proteins are called

A)sterilants.
B)oxidants.
C)detergents.
D)soaps.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
43
Antiseptics are generally regulated by the

A)Food and Drug Administration.
B)Environmental Protection Agency.
C)The National Institutes of Health.
D)Centers for Disease Control and Prevention.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
44
Although heavy metals are no longer widely used as germicides,__________ is an effective algicide in lakes and swimming pools.

A)lead acetate
B)copper sulfate
C)silver nitrate
D)mercuric chloride
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
45
Bacteriophage therapy was developed after antibiotics were first discovered and used for treating bacterial infections.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
46
The disinfectant of choice for municipal water supplies is __________.

A)fluorine
B)chlorine
C)either fluorine or chlorine
D)ultraviolet light
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
47
The disinfectant action of phenol and phenolic derivatives mainly is due to

A)its inherent detergent action.
B)membrane damage and protein denaturation.
C)oxidation of disulfide bonds in proteins.
D)extraction of lipids from membranes.
E)damage to nucleic acids and proteins caused by free radicals.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
48
The disinfecting properties of Lysol,a common household disinfectant,depend upon the presence of __________.
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Unlock Deck
k this deck
49
The disinfectant screening method that is known as the ________ _________ test,which is used to measure the potency of a disinfectant.
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Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
50
Iodine can be complexed with an organic carrier to form water-soluble,stable complexes called __________,which release iodine slowly and eliminate skin burns and irritation associated with iodine use.

A)iodides
B)tinctures
C)iodophors
D)iodochromes
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
51
Heavy metals are effective antimicrobial agents but are not widely used because of their high toxicity to humans.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
52
The two most important alcohol germicides are __________ and __________.

A)ethanol; methanol
B)ethanol; isopropanol
C)methanol; butanol
D)methanol; isopropanol
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
53
Alcohols are widely used as antiseptics and disinfectants because they are effective against endospores as well as vegetative cells.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
54
Which of the following is (are)a sterilizing gas(es)that can be used on heat-sensitive materials?

A)ethylene oxide
B)glutaraldehyde
C)chlorine gas
D)isopropanol
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
55
Disinfectants are generally regulated by the

A)Food and Drug Administration.
B)Environmental Protection Agency.
C)The National Institutes of Health.
D)Centers for Disease Control and Prevention.
Unlock Deck
Unlock for access to all 56 flashcards in this deck.
Unlock Deck
k this deck
56
Betapropiolactone is not as useful as ethylene oxide as a sterilizing agent because it does not penetrate materials as readily as ethylene oxide.
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Unlock for access to all 56 flashcards in this deck.