Deck 20: Genomics I: Analysis of Dna

Question 1

A site that has variation within the members of the population is said to be .

A) Monomorphic
B) Polymorphic
C) Trimorphic
D) None of the answers are correct

Answer 1

B

Question 2

What does physical mapping provide that the other types of genetic mapping do not?

A) Recombination distances between genes
B) Number of nucleotide base-pairs between genes
C) Relationship of the gene to specific chromosomal banding patterns
D) All of the answers are correct

Answer 2

B

Question 3

The most common micro satellite in humans is .

A) (AT)100
B) (CA)n
C) (TG)n
D) (CAT)n
E) None of the answers are correct

Answer 3

B

Question 4

The human genome project's first draft took approximately years.

A) 5
B) 13
C) 20
D) 50
E) 2

Question 5

Which of the following is NOT true regarding YACs?

A) YAC stands for yeast artificial cosmid.
B) They are used to clone large sections of eukaryotic chromosomes.
C) They contain DNA fragments much larger than conventional vectors.
D) They make it easier to construct a contig of a chromosome.
E) All of the above are true.

Question 6

If a lod score method indicates that two genes are linked the nucleotide distance between the genes
can be determined by determining the frequency of recombination between the genes.

Question 7

uses the unique banding patterns of a chromosome to determine the general location of a gene.

A) Physical mapping
B) Cytogenetic mapping
C) Linkage mapping
D) All of the answers are correct

Question 8

A map unit distance always corresponds to 106 base pairs.

Question 9

Which of the following is not a model organism under study by the Human Genome Project?

A) Drosophila melanogaster
B) Homo sapiens
C) Escherichia coli
D) Arabidopsis thalania
E) All of the answers are currently being studied

Question 10

Which of the following procedures best determines the relative order, but not precise location, of a series of genes on a chromosome?

A) Physical mapping
B) Cytogenetic mapping
C) Linkage mapping
D) All of the answers are correct

Question 11

If all members of a population have the identical DNA segment, it is said to be .

A) Monomorphic
B) Polymorphic
C) Trimorphic
D) None of the answers are correct

Question 12

A lod score of is accepted by most geneticists to indicate that two genes are linked.

A) 0
B) 1
C) 2
D) 3
E) 4

Question 13

The method of sequencing genomic fragments cloned straight from the genome is:

A) Cytogenetic mapping
B) Whole genome shotgun sequencing
C) Hierarchical shotgun sequencing
D) Southern blotting
E) Dideoxy blotting

Question 14

Which of the following may be used to clone a gene based upon its mapped position along a chromosome?

A) Sub cloning
B) Chromosome walking
C) Positional cloning
D) All of the answers are correct

Question 15

An RFLP marker may be used to predict the likelihood that an individual carries a trait for a specific
disease.

Question 16

The process of in situ hybridization is used for which of the following ?

A) Cytogenetic mapping
B) Physical mapping
C) Linkage mapping
D) None of the answers are correct

Question 17

Which of the following terms best describes how the components of a genome interact to produce an organism's traits?

A) Proteomics
B) Functional genomics
C) Structural genomics
D) Genomics

Question 18

Which of the following is a goal of the Human Genome Project?

A) To develop technological advances in molecular genetics
B) To analyze the genomes of model organisms
C) To obtain a physical map of the human genome
D) To obtain the DNA sequence of the human genome
E) All of the answers are correct

Question 19

Which of the following would generally not be used as a marker in a linkage mapping experiment?

A) RFLPs
B) VNTRs
C) STRs
D) FISH
E) STS

Question 20

involves an examination of how the proteins encoded by genes interact to produce cell and tissue types.

A) Proteomics
B) Functional genomics
C) Structural genomics
D) Genomics

Question 21

An amplified restriction fragment length polymorphism is a combination of the procedures for
producing RFLP and STS markers.

Question 22

A is a combination of a plasmid vector and phage I.

A) contig
B) artificial chromosome
C) cosmid
D) positional clone
E) transposable element

Question 23

Comparative genomics attempts to define evolutionary relationships.

Question 24

Bioinformatics refers to the molecular analysis of the entire genome of a species.

Question 25

In a FISH procedure, the probe being used is labeled with a fluorescent tag.

Question 26

The human genome consists of approximately base pairs of DNA.

A) 100,000
B) 1 million
C) 1 billion
D) 3 billion
E) 2 trillion

Question 27

A series of clones that contain overlapping pieces of a chromosome are called a .

A) contig
B) artificial chromosome
C) cosmid
D) positional clone
E) transposable element

Question 28

BAC stands for bacterial artificial chromosome.

Question 29

Cytogenetic mapping is accurate to within 500,000 base pairs, but not over this value.

Question 30

RFLP stands for restriction fragment linked position.

Question 31

The likelihood of linkage between two genes is determined using a analysis.

A) chi-square
B) linkage
C) shotgun
D) binomial expansion
E) lod score method