Deck 6: Microbial Viability and Growth

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Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Turbidity
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Question
_____________ is the basic building block for living organisms on Earth.​
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Growth factors
Question
The time it takes for a bacterial colony to divide and double its population size is called ________________ or doubling time.​
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Centrifuge
Question
The temperature that is most favorable for bacterial growth is considered its ________________ temperature.​
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Mesophiles
Question
How many phases are there in bacterial growth?​

A)​Two
B)​Three
C)​Four
D)​Five
Question
Which direct counting method is reported in terms of colony forming units (CFUs)?​

A)​Direct count
B)​Filtration
C)​Plate count
D)​Most probable number
Question
______________________ are often used to plot large numbers in a graphic bacterial population growth curve rather than arithmetically plotting the information.​
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Hypotonic
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Quorum sensing
Question
Which of the following methods of bacterial counts is considered indirect?​

A)​Direct count​
B)Filtration​
C)​Plate count
D)​Turbidity
Question
Obligate ________________ are microbes which cannot tolerate molecular oxygen.​
Question
In which stage of microbial growth is there no division seen for a period of one hour to several days?​

A)​Lag
B)​Log
C)​Death
D)​Stationary
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Suspension
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Biofilm
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Nucleotides
Question
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Chemostats
Question
Formation of biofilms is primarily dependent on the ability of bacteria for:​

A)​Colony growth
B)​Generation time
C)​Osmotic pressure
D)​Quorum sensing
Question
Approximately 50% of the dry weight of a bacterium is carbon.​
Question
An example of a solute is water and an example of a solvent is salt.​
Question
Substances with a pH of 6 or lower are termed alkaline.​
Question
Bioleaching is a method of removing microbial contamination from soil and plants.​
Question
Salted fish and meats are preserved and kept free of microbial growth by plasmolysis.​
Question
Second intention healing is characterized by the filling in of a wound with granulation tissue from the bottom up.​
Question
Facultative anaerobes produce energy more efficiently in the absence of oxygen.​
Question
Smelting is the process of extracting metals from ore by heat or chemical refining.​
Question
Pheromones are chemical signaling molecules used in quorum sensing of bacteria and also known as autoinducers.​
Question
E.coli is a species of bacteria which do not require additional growth factors.​
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Deck 6: Microbial Viability and Growth
1
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Turbidity
b
2
_____________ is the basic building block for living organisms on Earth.​
Carbon​
3
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Growth factors
e
4
The time it takes for a bacterial colony to divide and double its population size is called ________________ or doubling time.​
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5
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Centrifuge
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6
The temperature that is most favorable for bacterial growth is considered its ________________ temperature.​
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7
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Mesophiles
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8
How many phases are there in bacterial growth?​

A)​Two
B)​Three
C)​Four
D)​Five
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9
Which direct counting method is reported in terms of colony forming units (CFUs)?​

A)​Direct count
B)​Filtration
C)​Plate count
D)​Most probable number
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10
______________________ are often used to plot large numbers in a graphic bacterial population growth curve rather than arithmetically plotting the information.​
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11
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Hypotonic
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12
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Quorum sensing
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13
Which of the following methods of bacterial counts is considered indirect?​

A)​Direct count​
B)Filtration​
C)​Plate count
D)​Turbidity
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14
Obligate ________________ are microbes which cannot tolerate molecular oxygen.​
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15
In which stage of microbial growth is there no division seen for a period of one hour to several days?​

A)​Lag
B)​Log
C)​Death
D)​Stationary
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16
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Suspension
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17
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Biofilm
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18
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Nucleotides
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19
Match the following terms or phrases with the correct description or definition.​
a.Devices which allow bacterial cultures to be grown at a steady rate by removing waste products and adding fresh nutrients.
b.The amount of cloudiness present of a bacterial population in liquid media.
c.Groups of chemical compounds which, when linked together form the building blocks of DNA or RNA.
d.A solution with osmotic pressure lower than inside a cell, causing cell to swell with fluid.
e.Essential organic compounds which cannot be synthesized by some microbes so must be obtained from the surrounding environment.
f.Ability of bacteria to communicate by way of secreted factors such as Autoinducers and behave as a cohesive group rather than as individuals.
g.A laboratory device which spins specimens to separate fluids of differing density or liquids from solids.
h.An adherent layer created by a multicellular group bound by an extracellular matrix produced by the colony.
i.Microbes with optimum temperature of 98.6° F or 37° C, normal human body temperature.
j.A mixture in which solid particles are mixed into and spread throughout a liquid medium.
​Chemostats
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20
Formation of biofilms is primarily dependent on the ability of bacteria for:​

A)​Colony growth
B)​Generation time
C)​Osmotic pressure
D)​Quorum sensing
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21
Approximately 50% of the dry weight of a bacterium is carbon.​
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22
An example of a solute is water and an example of a solvent is salt.​
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23
Substances with a pH of 6 or lower are termed alkaline.​
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24
Bioleaching is a method of removing microbial contamination from soil and plants.​
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25
Salted fish and meats are preserved and kept free of microbial growth by plasmolysis.​
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26
Second intention healing is characterized by the filling in of a wound with granulation tissue from the bottom up.​
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27
Facultative anaerobes produce energy more efficiently in the absence of oxygen.​
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28
Smelting is the process of extracting metals from ore by heat or chemical refining.​
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29
Pheromones are chemical signaling molecules used in quorum sensing of bacteria and also known as autoinducers.​
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30
E.coli is a species of bacteria which do not require additional growth factors.​
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