Deck 13: Flow Cytometry

Question 1

Laser is an acronym for

A)light amplification by stimulated emission of radiation.
B)light augmentation by stimulated emitted radiation.
C)light amplified by stimulated energy radiation.
D)large angle stimulation by emitted radiation.

Answer 1

light amplification by stimulated emission of radiation.

Question 2

A photon is a

A)basic unit of light.
B)basic unit of all radiation.
C)component of an atom.
D)component of laser light.

Answer 2

basic unit of all radiation.

Question 3

Match the activity with the appropriate phase of testing related to laboratory automation.
Documentation of quality control data

A)Preanalytical
B)Analytical
C)Postanalytical

Answer 3

Postanalytical

Question 4

Laser light is concentrated.

Question 5

Lasers sort the energy in atoms and molecules, concentrate it, and release it in powerful waves.

Question 6

The most frequently used fluorochrome(s) is (are)

A)fluorescein isothiocyanate (FITC).
B)phycoerythrin (PE).
C)peridinin chlorophyll protein (PerCP).
D)All of the above.

Question 7

Blood specimens for flow cytometry analysis should be refrigerated.

Question 8

Match the activity with the appropriate phase of testing related to laboratory automation.
Specimen labeling

A)Preanalytical
B)Analytical
C)Postanalytical

Question 9

Match the activity with the appropriate phase of testing related to laboratory automation.
Validation of results

A)Preanalytical
B)Analytical
C)Postanalytical

Question 10

The major application(s) of flow cell technology is (are)

A)identification of cells.
B)cell sorting before further analysis.
C)diagnosis of autoimmune disease.
D)Both A and B.

Question 11

Tissue specimens require disaggregation before analysis by flow cytometry.

Question 12

Current fluorescent methods can perform up to _______-color analysis.

A)two
B)four
C)eight
D)sixteen

Question 13

A fluorochrome is a(n)

A)dye.
B)immunologic reagent.
C)nonspecific indicator.
D)Both A and B.

Question 14

The most common type of laser light used in laboratory instrumentation is neon.

Question 15

The principle of flow cytometry is based on

A)antigen-antibody reactions.
B)the formation of immune complexes.
C)the reaction of dyes with the cellular component of interest.
D)the reaction of dyes with immune complexes.

Question 16

Laser light is multicolored.

Question 17

Immunophenotyping includes the detection or identification of

A)CD34+ stem cells.
B)minimal residual disease in patients with leukemia.
C)T and B lymphocytes.
D)All of the above.

Question 18

Match the activity with the appropriate phase of testing related to laboratory automation.
Quality control

A)Preanalytical
B)Analytical
C)Postanalytical