Deck 11: Electrophoresis Techniques

Question 1

When comparing immunoelectrophoresis, IEP, and immunofixation electrophoresis, IFE, the following characteristics are true of IFE except that it is

A)more complex.
B)more sensitive.
C)used for difficult to characterize anomalous proteins.
D)challenging.

Answer 1

challenging.

Question 2

IFE results are interpreted visually, by inspection of the bands produced. Characteristics of normal bands on IFE include all of the following except:

A)bands are broad up and down the lane.
B)narrow dense bands with sharp borders are present.
C)gradual reduction in color intensity toward edges of the band.
D)smooth reduction in intensity of band toward edges of the band.

Answer 2

narrow dense bands with sharp borders are present.

Question 3

A monoclonal gammopathy is

A)frequently diagnosed using Immunofixation electrophoresis (IFE).
B)ruled out in a diagnosis of MGUS.
C)ruled out in the case of negative results for IFE.
D)ruled out in the case of negative results for serum and urine protein electrophoresis.

Answer 3

frequently diagnosed using Immunofixation electrophoresis (IFE).

Question 4

Normal results for serum IFE include

A)darker IgG lane.
B)lighter IgA lane.
C)absent IgM lane.
D)darker kappa lane compared to lambda lane.
E)All the above.

Question 5

A polyclonal pattern observed in IFE results for serum or urine is considered

A)specific for a monoclonal protein (M-protein).
B)nonspecific.
C)to exhibit increased intensity of color in one or two lanes.
D)to exclude the possibility of the presence of an M-protein.

Question 6

The most commonly identified M-protein in serum IFE is

A)IgG.
B)IgA.
C)IgM.
D)IgD.

Question 7

In addition to IFE, other techniques for the identification of M proteins include

A)capillary zone electrophoresis.
B)isotachophoresis.
C)capillary isoelectric focusing.
D)All of the above.

Question 8

Separation of proteins by electrophoresis is based on

A)rate of migration of individual components in an electrical field.
B)size of various molecules as they diffuse on cellulose acetate.
C)the type of support medium such as cellulose acetate.
D)All of the above.

Question 9

Electrophoresis is defined as

A)migration of charged solutes in an electrical field.
B)migration of charged particles in an electrical field.
C)diffusion of charged particles in an electrical field.
D)Both A and B.

Question 10

Immunofixation Electrophoresis (IFE) is a reliable and accurate method for detecting

A)multiple myeloma.
B)Waldenstrom's Macroglobulinemia.
C)amyloid light chain amyloidosis.
D)All the above.

Question 11

Suitable samples for Immunofixation Electrophoresis (IFE) include

A)serum.
B)urine.
C)cerebrospinal fluid.
D)other body fluids.
E)All the above.

Question 12

In the first phase of Immunofixation Electrophoresis (IFE), serum is separated according to

A)electrophoretic mobility.
B)diffusion coefficient.
C)antibody specificity.
D)All of the above.

Question 13

Advantages of Microchip capillary electrophoresis include

A)high speed.
B)reduced reagent consumption.
C)integration analysis.
D)All of the above.