Deck 2: Living Cells

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Question
During exocytosis membrane-bound vesicles migrate from the Golgi apparatus to the __________.

A) Lysosome
B) Smooth endoplasmic reticulum
C) Cell wall
D) Plasma membrane
E) More than one answer is correct
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Question
Notable features of eukaryotes include all of the following except ________.

A) Rapid cell division
B) Larger size
C) A complex internal structure
D) An endomembrane system
E) Organelles
Question
Gram-negative bacteria lack _________.

A) Cell walls
B) Peptidoglycans in the cell wall
C) An outer coat of lipid bound proteins
D) DNA
E) Carbohydrate containing polymers in the cell wall
Question
The plasma membrane _________.

A) Provides support
B) Is present only in eukaryotes
C) Controls the flow of substances in and out of the cell
D) Is present only in prokaryotes
E) Both A and C are correct
Question
Structural features that make phospholipids suited for their biological role are:

A) A hydrophilic head group
B) Branched carbon chains
C) A hydrophobic group
D) A and C are correct
E) None of the above is true
Question
The grainy appearance of prokaryotic cytoplasm is due in part to ________.

A) A large number of ribosomes
B) Plasmids
C) Nucleolus
D) DNA
E) RNA
Question
Which of the following types of molecules is not hydrophilic

A) Sodium chloride
B) Glucose
C) Amino acids
D) Fats
E) Both A and B are correct.
Question
The basic structure of biological membranes is provided by what class of compounds?

A) Proteins
B) Lipids
C) Carbohydrates
D) Steroids
E) Peptidoglycans
Question
Which of the following is not true of the nucleus?

A) It is found in both eukaryotes and prokaryotes
B) It contains the cell's "genetic blueprint"
C) It exerts a profound influence over all cellular metabolic functions
D) It contains chromatin fibers
E) It is bounded by membrane
Question
Which of the following statements is not true of the endoplasmic reticulum?

A) It often constitutes more than half of the cell's total membrane
B) It exists in two forms: rough and smooth
C) Rough ER is responsible for protein synthesis
D) Smooth ER is responsible for lipid synthesis
E) Rough ER ribosomes are responsible for biotransformation.
Question
Which of the following is not true of eukaryotic ribosomes?

A) They are macromolecular machines
B) They function in the biosynthesis of proteins
C) They contain ribosomal RNA
D) They consist of two unequally sized subunits
E) They are attached to the smooth ER
Question
Which of the following statements is not true of the Golgi apparatus?

A) The analogous plant structure is referred to as a dictyosome
B) It has two faces
C) The cisterna or forming face usually projects toward the ER
D) It is involved in the packaging and secretion of cell products
E) It is usually located near the plasma membrane
Question
Which of the following statements is not true of the lysosome?

A) It functions in intercellular digestion
B) Both ER and Golgi apparatus are involved in its formation
C) It is capable of degrading most biomolecules
D) It is involved in the packaging and secretion of cell products
E) It is involved in pathological conditions such as gout and rheumatoid arthritis
Question
Acid hydrolases are found in ___________.

A) Ribosomes
B) Lysosomes
C) Golgi Apparatus
D) Dictyosomes
E) Smooth ER
Question
The main role of peroxisomes is _____________.

A) Anaerobic respiration
B) Aerobic respiration
C) Lipid oxidation
D) Generation and destruction of peroxides
E) Carbohydrate oxidation
Question
Biological machines are derived from which class of compounds

A) Fats
B) Proteins
C) Carbohydrates
D) Ribosomes
E) B and C
Question
Which of the following organelles is involved in aerobic metabolism?

A) Mitochondria
B) Peroxisomes
C) Glyoxysomes
D) Ribosomes
E) Lysosomes
Question
Respiratory assemblies are responsible for the synthesis of ___________.

A) ATP
B) NADH
C) GTP
D) Hydrogen peroxide
E) ADP
Question
The two membranes of the mitochondria create two separate compartments called the intermembrane space and the __________.

A) Cristae
B) Matrix
C) Lumen
D) Cisternae
E) Vesicle
Question
Which of the following statements is not true of the mitochondrial matrix?

A) It contains enzymes responsible for nucleotide metabolism
B) It contains circular DNA molecules
C) It contains all of the necessary compounds for protein synthesis
D) It has a gel-like consistency
E) It has a high concentration of enzymes and ions
Question
The excluded volume of a cell varies between _____ and ______%.

A) 5-10
B) 10-20
C) 20-40
D) 40-50
E) 0-5
Question
Plastids are plant structures similar to what organelle found in animals?

A) Ribosomes
B) Leucoplasts
C) Golgi apparatus
D) Peroxisomes
E) None of the above is correct
Question
The stroma of plastids is analogous to what structure of mitochondria?

A) Grana
B) Matrix
C) Inner membrane
D) Outer membrane
E) Cisternal space
Question
Which of the following help make up the cytoskeleton?

A) Microtubules
B) Microfilaments
C) Intermediate fibers
D) B and C are correct
E) All are correct
Question
Mitochondria and _________ consume molecular oxygen.

A) Plastids
B) Ribosomes
C) Peroxisomes
D) Lysosome
E) Golgi apparatus
Question
Which of the following is present only in prokaryotic cells?

A) Nucleus
B) Endoplasmic reticulum
C) Plasma membrane
D) Nucleolus
E) None of the above is correct
Question
Biotransformation is defined as:

A) The preparation of water-insoluble substances for excretion
B) Oxidation of organic molecules
C) Transformation of food molecules to ATP
D) Reactions occurring within a living system
E) Conversion of harmless compounds to toxins
Question
In place of a nucleus a prokaryotic cell has a __________.

A) Mesosome
B) Nucleoid
C) Centrosome
D) Nucleosome
E) Reticulosome
Question
Which of the following eukaryotic organelles is a key regulator of apoptosis?

A) Mitochondria
B) Flagella
C) Chloroplasts
D) Cilia
E) Ribosomes
Question
Which of the following is not a role of transmembrane proteins?

A) Transport of ions
B) Structural support
C) Transport of nutrients
D) Receptors
E) Enzymes
Question
Which of the following is not a membrane protein?

A) Channel protein
B) Carrier protein
C) Receptors
D) Integral proteins
E) Motor proteins
Question
Examples of eukaryotic signal molecules are:

A) Neurotransmitters
B) Hormones
C) Cytokines
D) Both A and B are correct
E) All of the above are correct
Question
Which of the following is not a phase of the information processing mechanism?

A) Reception
B) Transduction
C) Response
D) Transformation
E) Both A and C are correct
Question
Which of the following is not a component of the endomembrane system?

A) Plasma membrane
B) Endoplasmic reticulum
C) Golgi apparatus
D) Lysosomes
E) Ribosomes
Question
The carbohydrate coat of a cell is called the:

A) Extracellular matrix
B) Glycocalyx
C) Cell cortex
D) Fibroblast
E) Both A and C are correct
Question
The perinuclear space is:

A) The space between the nucleus and the cytoplasm
B) The space between the membranes of the nuclear envelope
C) The space between the nucleus and the nucleous
D) The space between DNA and histones
E) Both A and B are correct
Question
Which of the following organelles are involved in autophagy?

A) Mitochondria
B) Lysosomes
C) Chloroplasts
D) Golgi apparatus
E) Both B and C are correct
Question
Which of the following molecules is not an example of a type of eukaryotic signal molecule?

A) Neurotransmitter
B) Hormone
C) Cytokinin
D) Endotoxin
E) Both A and B are correct
Question
Of the approximately100 trillion cells in the human body how many are actually human cells?

A) All of them
B) 50 Trillion
C) 90 Trillion
D) 10 Trillion
E) 80 Trillion
Question
Analysis of the RNA of prokaryotes reveals that there are ______ distinct types of
Prokaryotes.

A) One
B) Two
C) Three
D) Four
E) Five
Question
Which of the following are microbiota

A) Bacteria
B) Fungi
C) Archea
D) A and B
E) A, B and C
Question
Which of the following are techniques used by the body to protect itself against
Indigenous microbiota

A) Impenetrable epithelial tissue
B) Immune system cells
C) Antimicrobial proteins
D) Beta defensins
E) All of the above
Question
Which of the following is a superorganism

A) Streptococcus
B) Lactobacillus
C) Homo sapiens
D) Corynebacterium
E) Klebsiella
Question
The endocytic cycle is

A) A means of remodeling plasma membrane
B) A form a signal transduction
C) Cycling of sodium and potassium ions through the cell membrane
D) Continuous recycling of membrane through endocytosis and exocytosis
E) A and D
Question
What is an integral protein?
Question
What is a peripheral protein?
Question
What is a ligand?
Question
What is the function of a motor protein?
Question
What is meant by the term macromolecular crowding?
Question
What is an endotoxin?
Question
What is the slime layer?
Question
Explain why the term "crowded" rather than "concentrated" is used to describe the densely packed molecules on the interior of living cells?
Question
Describe how noncovalent interactions promote the self-assembly of supramolecular structures in living organisms.
Question
How do lysosomes participate in the life of the cell?
Question
What functions does the cytoskeleton perform in living cells?
Question
What roles do plasma membrane proteins play in living cells?
Question
What are two essential functions of the nucleus?
Question
Describe the functions of the Golgi apparatus.
Question
List three environmental signals detected by primary cilia.
Question
Describe the function of kinesins and dyneins in intraflagellar transport.
Question
Humans have been described as superorganisms consisting of human cells in combination with a larger number of microorganisms. Explain. Is this a symbiotic relationship? What effect can the consumption of antibiotics have on this relationship?
Question
Biological membranes are held together by weak noncovalent forces. Why is it important that weak forces hold membranes together? What would happen if membrane structure involved stronger covalent bonding?
Question
What effect does macromolecular crowding have on the traditional analysis of reaction rates?
Question
Eukaryotic cells are much larger cells than prokaryotic cells. What factors of internal structure make this increase in size possible?
Question
Comment on the statement that the cytoplasm is just a soup containing organelles.
Question
The interior of living cells is highly organized and this organization is maintained over long periods of time. Entropy, a measure of the disorder or randomness of the system, predicts that highly organized systems should become progressively disorganized. How does a living cell evade entropy?
Question
The surface of the eukaryotic cell is coated with carbohydrate groups attached to protein and lipid molecules. What structural feature of the carbohydrate molecule accounts for this phenomenon?
Question
Mitochondria are one of many types of organelles. What features of mitochondria argue most strongly for their descent from a free living ancestor?
Question
In eukaryotes the DNA remains within the nucleus and RNA, synthesized within the nucleus, is used to transfer information to the rest of the cell. Why use RNA for this purpose and why not just use DNA directly?
Question
In density gradient centrifugation the products are removed from the bottom of the centrifuge tube. Why not remove them from the top of the tube?
Question
The dimensions of a prokaryotic ribosome are approximately 14 nm x 20 nm. If ribosomes occupy 20% of the volume of a bacterial cell calculate how many ribosomes are in a typical cell such as E. coli. Assume that the shape of a ribosome is approximately that of a cylinder. A typical bacterial cell volume is 1.6 um3.
Question
The E. coli cell is 2 m long and 1 m in diameter, while a typical eukaryotic cell is 20 m in diameter. Assuming that the E. coli cell is a perfect cylinder and the eukaryotic cell is a perfect sphere, calculate the surface-to-volume ratio for each cell type [cylinder volume V = r2h; cylinder area A = 2r2 + 2rh; sphere volume V = 4/3(r3); sphere area 4r2].What do these numbers tell you about the evolutionary changes that would have to occur to generate an efficient eukaryotic cell considering that most biochemical processes depend on membrane-bound processes.
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Deck 2: Living Cells
1
During exocytosis membrane-bound vesicles migrate from the Golgi apparatus to the __________.

A) Lysosome
B) Smooth endoplasmic reticulum
C) Cell wall
D) Plasma membrane
E) More than one answer is correct
D
2
Notable features of eukaryotes include all of the following except ________.

A) Rapid cell division
B) Larger size
C) A complex internal structure
D) An endomembrane system
E) Organelles
A
3
Gram-negative bacteria lack _________.

A) Cell walls
B) Peptidoglycans in the cell wall
C) An outer coat of lipid bound proteins
D) DNA
E) Carbohydrate containing polymers in the cell wall
E
4
The plasma membrane _________.

A) Provides support
B) Is present only in eukaryotes
C) Controls the flow of substances in and out of the cell
D) Is present only in prokaryotes
E) Both A and C are correct
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k this deck
5
Structural features that make phospholipids suited for their biological role are:

A) A hydrophilic head group
B) Branched carbon chains
C) A hydrophobic group
D) A and C are correct
E) None of the above is true
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
6
The grainy appearance of prokaryotic cytoplasm is due in part to ________.

A) A large number of ribosomes
B) Plasmids
C) Nucleolus
D) DNA
E) RNA
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
7
Which of the following types of molecules is not hydrophilic

A) Sodium chloride
B) Glucose
C) Amino acids
D) Fats
E) Both A and B are correct.
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
8
The basic structure of biological membranes is provided by what class of compounds?

A) Proteins
B) Lipids
C) Carbohydrates
D) Steroids
E) Peptidoglycans
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
9
Which of the following is not true of the nucleus?

A) It is found in both eukaryotes and prokaryotes
B) It contains the cell's "genetic blueprint"
C) It exerts a profound influence over all cellular metabolic functions
D) It contains chromatin fibers
E) It is bounded by membrane
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
10
Which of the following statements is not true of the endoplasmic reticulum?

A) It often constitutes more than half of the cell's total membrane
B) It exists in two forms: rough and smooth
C) Rough ER is responsible for protein synthesis
D) Smooth ER is responsible for lipid synthesis
E) Rough ER ribosomes are responsible for biotransformation.
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
11
Which of the following is not true of eukaryotic ribosomes?

A) They are macromolecular machines
B) They function in the biosynthesis of proteins
C) They contain ribosomal RNA
D) They consist of two unequally sized subunits
E) They are attached to the smooth ER
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
12
Which of the following statements is not true of the Golgi apparatus?

A) The analogous plant structure is referred to as a dictyosome
B) It has two faces
C) The cisterna or forming face usually projects toward the ER
D) It is involved in the packaging and secretion of cell products
E) It is usually located near the plasma membrane
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
13
Which of the following statements is not true of the lysosome?

A) It functions in intercellular digestion
B) Both ER and Golgi apparatus are involved in its formation
C) It is capable of degrading most biomolecules
D) It is involved in the packaging and secretion of cell products
E) It is involved in pathological conditions such as gout and rheumatoid arthritis
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
14
Acid hydrolases are found in ___________.

A) Ribosomes
B) Lysosomes
C) Golgi Apparatus
D) Dictyosomes
E) Smooth ER
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Unlock Deck
k this deck
15
The main role of peroxisomes is _____________.

A) Anaerobic respiration
B) Aerobic respiration
C) Lipid oxidation
D) Generation and destruction of peroxides
E) Carbohydrate oxidation
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Unlock Deck
k this deck
16
Biological machines are derived from which class of compounds

A) Fats
B) Proteins
C) Carbohydrates
D) Ribosomes
E) B and C
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Unlock Deck
k this deck
17
Which of the following organelles is involved in aerobic metabolism?

A) Mitochondria
B) Peroxisomes
C) Glyoxysomes
D) Ribosomes
E) Lysosomes
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Unlock Deck
k this deck
18
Respiratory assemblies are responsible for the synthesis of ___________.

A) ATP
B) NADH
C) GTP
D) Hydrogen peroxide
E) ADP
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
19
The two membranes of the mitochondria create two separate compartments called the intermembrane space and the __________.

A) Cristae
B) Matrix
C) Lumen
D) Cisternae
E) Vesicle
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
20
Which of the following statements is not true of the mitochondrial matrix?

A) It contains enzymes responsible for nucleotide metabolism
B) It contains circular DNA molecules
C) It contains all of the necessary compounds for protein synthesis
D) It has a gel-like consistency
E) It has a high concentration of enzymes and ions
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
21
The excluded volume of a cell varies between _____ and ______%.

A) 5-10
B) 10-20
C) 20-40
D) 40-50
E) 0-5
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
22
Plastids are plant structures similar to what organelle found in animals?

A) Ribosomes
B) Leucoplasts
C) Golgi apparatus
D) Peroxisomes
E) None of the above is correct
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
23
The stroma of plastids is analogous to what structure of mitochondria?

A) Grana
B) Matrix
C) Inner membrane
D) Outer membrane
E) Cisternal space
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Unlock Deck
k this deck
24
Which of the following help make up the cytoskeleton?

A) Microtubules
B) Microfilaments
C) Intermediate fibers
D) B and C are correct
E) All are correct
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Unlock Deck
k this deck
25
Mitochondria and _________ consume molecular oxygen.

A) Plastids
B) Ribosomes
C) Peroxisomes
D) Lysosome
E) Golgi apparatus
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
26
Which of the following is present only in prokaryotic cells?

A) Nucleus
B) Endoplasmic reticulum
C) Plasma membrane
D) Nucleolus
E) None of the above is correct
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
27
Biotransformation is defined as:

A) The preparation of water-insoluble substances for excretion
B) Oxidation of organic molecules
C) Transformation of food molecules to ATP
D) Reactions occurring within a living system
E) Conversion of harmless compounds to toxins
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
28
In place of a nucleus a prokaryotic cell has a __________.

A) Mesosome
B) Nucleoid
C) Centrosome
D) Nucleosome
E) Reticulosome
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Unlock Deck
k this deck
29
Which of the following eukaryotic organelles is a key regulator of apoptosis?

A) Mitochondria
B) Flagella
C) Chloroplasts
D) Cilia
E) Ribosomes
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Unlock Deck
k this deck
30
Which of the following is not a role of transmembrane proteins?

A) Transport of ions
B) Structural support
C) Transport of nutrients
D) Receptors
E) Enzymes
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Unlock Deck
k this deck
31
Which of the following is not a membrane protein?

A) Channel protein
B) Carrier protein
C) Receptors
D) Integral proteins
E) Motor proteins
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Unlock Deck
k this deck
32
Examples of eukaryotic signal molecules are:

A) Neurotransmitters
B) Hormones
C) Cytokines
D) Both A and B are correct
E) All of the above are correct
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
33
Which of the following is not a phase of the information processing mechanism?

A) Reception
B) Transduction
C) Response
D) Transformation
E) Both A and C are correct
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Unlock Deck
k this deck
34
Which of the following is not a component of the endomembrane system?

A) Plasma membrane
B) Endoplasmic reticulum
C) Golgi apparatus
D) Lysosomes
E) Ribosomes
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Unlock Deck
k this deck
35
The carbohydrate coat of a cell is called the:

A) Extracellular matrix
B) Glycocalyx
C) Cell cortex
D) Fibroblast
E) Both A and C are correct
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
36
The perinuclear space is:

A) The space between the nucleus and the cytoplasm
B) The space between the membranes of the nuclear envelope
C) The space between the nucleus and the nucleous
D) The space between DNA and histones
E) Both A and B are correct
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
37
Which of the following organelles are involved in autophagy?

A) Mitochondria
B) Lysosomes
C) Chloroplasts
D) Golgi apparatus
E) Both B and C are correct
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
38
Which of the following molecules is not an example of a type of eukaryotic signal molecule?

A) Neurotransmitter
B) Hormone
C) Cytokinin
D) Endotoxin
E) Both A and B are correct
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
39
Of the approximately100 trillion cells in the human body how many are actually human cells?

A) All of them
B) 50 Trillion
C) 90 Trillion
D) 10 Trillion
E) 80 Trillion
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
40
Analysis of the RNA of prokaryotes reveals that there are ______ distinct types of
Prokaryotes.

A) One
B) Two
C) Three
D) Four
E) Five
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
41
Which of the following are microbiota

A) Bacteria
B) Fungi
C) Archea
D) A and B
E) A, B and C
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
42
Which of the following are techniques used by the body to protect itself against
Indigenous microbiota

A) Impenetrable epithelial tissue
B) Immune system cells
C) Antimicrobial proteins
D) Beta defensins
E) All of the above
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
43
Which of the following is a superorganism

A) Streptococcus
B) Lactobacillus
C) Homo sapiens
D) Corynebacterium
E) Klebsiella
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
44
The endocytic cycle is

A) A means of remodeling plasma membrane
B) A form a signal transduction
C) Cycling of sodium and potassium ions through the cell membrane
D) Continuous recycling of membrane through endocytosis and exocytosis
E) A and D
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
45
What is an integral protein?
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k this deck
46
What is a peripheral protein?
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47
What is a ligand?
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k this deck
48
What is the function of a motor protein?
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49
What is meant by the term macromolecular crowding?
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50
What is an endotoxin?
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51
What is the slime layer?
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52
Explain why the term "crowded" rather than "concentrated" is used to describe the densely packed molecules on the interior of living cells?
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k this deck
53
Describe how noncovalent interactions promote the self-assembly of supramolecular structures in living organisms.
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k this deck
54
How do lysosomes participate in the life of the cell?
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55
What functions does the cytoskeleton perform in living cells?
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56
What roles do plasma membrane proteins play in living cells?
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57
What are two essential functions of the nucleus?
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58
Describe the functions of the Golgi apparatus.
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59
List three environmental signals detected by primary cilia.
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60
Describe the function of kinesins and dyneins in intraflagellar transport.
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Unlock Deck
k this deck
61
Humans have been described as superorganisms consisting of human cells in combination with a larger number of microorganisms. Explain. Is this a symbiotic relationship? What effect can the consumption of antibiotics have on this relationship?
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Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
62
Biological membranes are held together by weak noncovalent forces. Why is it important that weak forces hold membranes together? What would happen if membrane structure involved stronger covalent bonding?
Unlock Deck
Unlock for access to all 72 flashcards in this deck.
Unlock Deck
k this deck
63
What effect does macromolecular crowding have on the traditional analysis of reaction rates?
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64
Eukaryotic cells are much larger cells than prokaryotic cells. What factors of internal structure make this increase in size possible?
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65
Comment on the statement that the cytoplasm is just a soup containing organelles.
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66
The interior of living cells is highly organized and this organization is maintained over long periods of time. Entropy, a measure of the disorder or randomness of the system, predicts that highly organized systems should become progressively disorganized. How does a living cell evade entropy?
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67
The surface of the eukaryotic cell is coated with carbohydrate groups attached to protein and lipid molecules. What structural feature of the carbohydrate molecule accounts for this phenomenon?
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68
Mitochondria are one of many types of organelles. What features of mitochondria argue most strongly for their descent from a free living ancestor?
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69
In eukaryotes the DNA remains within the nucleus and RNA, synthesized within the nucleus, is used to transfer information to the rest of the cell. Why use RNA for this purpose and why not just use DNA directly?
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70
In density gradient centrifugation the products are removed from the bottom of the centrifuge tube. Why not remove them from the top of the tube?
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71
The dimensions of a prokaryotic ribosome are approximately 14 nm x 20 nm. If ribosomes occupy 20% of the volume of a bacterial cell calculate how many ribosomes are in a typical cell such as E. coli. Assume that the shape of a ribosome is approximately that of a cylinder. A typical bacterial cell volume is 1.6 um3.
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72
The E. coli cell is 2 m long and 1 m in diameter, while a typical eukaryotic cell is 20 m in diameter. Assuming that the E. coli cell is a perfect cylinder and the eukaryotic cell is a perfect sphere, calculate the surface-to-volume ratio for each cell type [cylinder volume V = r2h; cylinder area A = 2r2 + 2rh; sphere volume V = 4/3(r3); sphere area 4r2].What do these numbers tell you about the evolutionary changes that would have to occur to generate an efficient eukaryotic cell considering that most biochemical processes depend on membrane-bound processes.
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