Deck 2: Laboratory Operations

Question 1

Earl understands that exposure to body fluids, tissue samples, and cells for processing, entails the observance of which of the following biosafety levels?

A)Biosafety Level 1
B)Biosafety Level 2
C)Biosafety Level 3
D)Biosafety Level 4

Answer 1

Biosafety Level 2

Question 2

Frank remembers that the correct set of personal protective equipment required for biosafety level 1 is which of the following:

A)Sterile gloves, lab gown, eye patch, and proper footwear
B)Working gloves, lab coat, goggles, and wooden sandals
C)Gloves, lab gown, eye protection, and appropriate footwear
D)Gloves, lab coat, eyeglasses, and proper footwear

Answer 2

Gloves, lab gown, eye protection, and appropriate footwear

Question 3

Gerald knows that among the following equipment, one of which protects the worker, the products stored and the environment.

A)Chemical fume hoods
B)Biosafety cabinets
C)Clean benches
D)Refrigerator

Answer 3

Biosafety cabinets

Question 4

Houston is correct when he identifies which among the following practices are correct in wearing gloves as a PPE, except:

A)Gloves are donned when handling biohazardous materials.
B)Nitrile gloves must be worn when processing materials of extreme temperatures.
C)Gloves must always overlap the lab coat sleeves to ensure protection.
D)The practice of "double gloving" provides more protection in some situations.

Question 5

Ice is correct when he practices which of the following principles when dealing with sharps in the laboratory?

A)Request removal of the sharps container when it is already almost full.
B)Needles can be recapped with the fingers of the other hand using extra precaution.
C)Always utilize a mechanical tool when removing knives, and other types of sharps.
D)Broken glassware can be handled directly using a double-gloved hands.

Question 6

Jay is correct when he adheres to the following recommended practices for handling human cells and tissues in the clinical laboratory

A)All work with such cells and tissues should be done in a biosafety cabinet.
B)All potentially infected samples should be decontaminated by autoclaving only.
C)All laboratory staff is not required to submit blood samples for reference.
D)All laboratory personnel should strictly follow the BSL-1 recommendation.

Question 7

Dexter understands that the following principles of biosafety are correct, except:

A)The primary goal of biosafety is to contain potentially infectious biological materials.
B)Containing potentially infectious agents is aimed at reducing exposure to these hazardous materials.
C)Utilization of vaccines on health care workers will surely protect against harmful pathogens.
D)Doing assessment of risks when handling potentially hazardous materials can be generalized with a group of specimens to increase efficiency.

Question 8

Joy knows that observation of the following laboratory practices is correct.

A)Containment is effective when standard microbiological procedures are often observed and followed.
B)Personnel working with potentially infectious agents should be aware of the potential hazards of handling such specimens.
C)Training on proper handling of potentially infected samples can be substituted by gaining experience in the laboratory.
D)The laboratory personnel are responsible for self training on such essential laboratory techniques and principles.

Question 9

Mark is correct to identify which among the following is not a form of safety equipment in the laboratory?

A)Biosafety cabinet
B)Enclosed containers
C)Fume hood
D)Engineering controls

Question 10

Noel knows that which among the following statements is true regarding biosafety cabinets.

A)There are four classes of biosafety cabinets used in the laboratories.
B)Both Class I and Class II biosafety cabinets are able to serve as primary barriers that protects both the lab workers and the environment.
C)Class II biosafety cabinets are not able to provide protection from external contamination of materials.
D)Class IV biosafety cabinets provide the greatest level of protection for both the lab personnel and the environment.

Question 11

Orlando knows that the following are primary barriers used inside the laboratory, except:

A)Safety centrifuge cup
B)Shoe covers
C)Reading glasses
D)Face shield

Question 12

Paul remembers correctly that which of the following is not a secondary barrier employed in the laboratory?

A)Biosafety cabinet
B)Division of the specific areas in the laboratory
C)Decontamination facility
D)Autoclave machine

Question 13

Quark is correct to identify which among the biosafety levels are applied when working with samples that might contain the following microorganisms: Bacillus subtilis, Nigeria gruberi, and infectious canine hepatitis virus?

A)BSL - 2
B)BSL - 3
C)BSL - 1
D)BSL - 4

Question 14

Roger understands that when dealing with lung tissue that may be infected with Mycobacterium tuberculosis, he must apply which of the following biosafety levels to adequately protect himself and the environment?

A)BSL - 4
B)BSL - 3
C)BSL - 2
D)BSL - 1

Question 15

Sylvester is correct when he replies that the following biosafety level is applicable inside the clinical laboratory when working with specimens that are potentially contaminated with pathogens that presents a moderate risk of causing human diseases such as the hepatitis B virus, HIV, and Salmonella species?

A)BSL - 1
B)BSL - 2
C)BSL - 3
D)BSL - 4

Question 16

Tony understands that sterilization in the clinical laboratory can be achieved by the following methods, except:

A)Heating
B)Radiation
C)Ethylene oxide gas
D)Plasma

Question 17

Uranus is correct when he states that which among the following is correct about the factors that affect the effectiveness of disinfection?

A)The number of non-pathogenic microorganisms
B)The volume of organic matter that is present during disinfection
C)The model or make of the materials to be disinfected
D)The humidity of the environment

Question 18

Voltaire is correct to identify that instruments that come in contact with sterile parts of the body must require sterilization and is classified under which of the following category?

A)Non critical items
B)Semi-critical items
C)Critical items
D)Extremely critical items

Question 19

William is right when he classifies items used in the laboratory that constantly comes into contact with the skin. These items can be disinfected with a low to intermediate level of disinfectant as well as by the use of soap and water. In which of the following are these types of items classified?

A)Non critical items
B)Semi-critical items
C)Critical items
D)Extremely critical items

Question 20

Xerxes understands correctly if he categorizes the instruments used inside the clinical laboratory that would require either sterilization or disinfection into which of the following classification?

A)Non critical items
B)Semi-critical items
C)Critical items
D)Extremely critical items

Question 21

Yam remembers correctly if he identifies which among the levels of disinfection is being described as being capable of sterilization if done for extended periods of time of up to 10 hours?

A)Low level disinfection
B)Intermediate level disinfection
C)High level disinfection
D)Sterilization

Question 22

Zack recalls that the level of disinfection that is able to kill proliferative microorganisms, all types of fungi, and is able to inactivate most species of viruses is which among the following levels?

A)Low level disinfection
B)Intermediate level disinfection
C)High level disinfection
D)Sterilization

Question 23

Ariel understands that the type of disinfection that is able to kill most species of bacteria, fungi, and viruses is which among the following categories?

A)Sterilization
B)High level disinfection
C)Low level disinfection
D)Intermediate level disinfection

Question 24

Brian remembers correctly if he identifies which among the following is not a purpose of decontamination in the clinical laboratory?

A)Make the instruments and devices safer to work with
B)Protects the personnel from infection
C)Prevents the contamination of the environment
D)Facilitates traffic inside the laboratory

Question 25

Carlo is right when he identifies which principle is applied when using steam sterilization on items that were not pre-cleaned?

A)Duration is prolonged and the temperature is increased.
B)Duration is prolonged but the temperature remains the same.
C)Duration is maintained but the temperature used is increased.
D)Both the duration and the temperature are kept the same way as sterilizing cleaned items

Question 26

Drake correctly identifies which among the following is the most resistant to disinfectants?

A)Spores of Bacillus species
B)Mycobacterium species
C)Fungi
D)Poliovirus

Question 27

Earl understands correctly that which among the following is the easiest to kill or inhibit when using disinfectants in the laboratory?

A)Mycobacterium tuberculosis
B)Candida albicans
C)Rhinovirus
D)Ebola virus

Question 28

Francis recalls correctly that which among the following substances are used in decontaminating large spaces inside the laboratory, except:

A)Formaldehyde gas
B)Hydrogen peroxide vapor
C)Glutaraldehyde vapor
D)Chlorine dioxide gas

Question 29

Gabin understands that he must apply which of the following precautions when dealing with blood samples and tissues that might have blood borne pathogens?

A)Standard precautions
B)Universal precautions
C)Contact precautions
D)Droplet precautions

Question 30

Harris is safely implementing infection control in the lab when he observes which of the following precautions when handling human tissues and organs before fixation?

A)Contact precaution
B)Standard precaution
C)Universal precaution
D)Airborne precaution

Question 31

The histotechnician is correct when he applies which of the following precaution when dealing with another laboratory staff who coughs and sneezes in front of him?

A)Standard precaution
B)Airborne precaution
C)Droplet precaution
D)Vector precaution

Question 32

The histology assistant understands the general principles of using personal protective equipment when he identifies which among the following is not correct?

A)Always perform hand hygiene before doing any personal protective equipment
B)Any damaged parts of are-usable personal protective equipment must be replaced immediately.
C)Removal of personal protective equipment can be delayed even after completing a procedure to save time and resources.
D)All single used personal protective equipment must be discarded properly and immediately after use.

Question 33

The histotechnician understands that the correct sequence of putting on personal protective equipment is done in which order?

A)Hand hygiene, mask, goggles, gown, gloves
B)Hand hygiene, gown, mask, goggles, gloves
C)Hand hygiene, gloves, mask, goggles, gown
D)Hand hygiene, gown, gloves, mask, goggles

Question 34

The microscope is one of the most important instruments used in the laboratory. It is therefore a must that a histotechnician is knowledgeable about how to take care of the microscope. When removing the oil in the lens of a microscope, what should a histotechnician do?

A)Use a damped lens paper with alcohol
B)Use a damped cloth with xylene
C)Use a damped cloth with alcohol
D)Use a damped lens paper with xylene

Question 35

Ben is correct when he disposes wastes in the laboratory that is dry and non infectious in which of the following trash bins?

A)Green
B)Yellow
C)Orange
D)Black

Question 36

Bing correctly disposes infectious, pathological wastes in which of the following trash bins?

A)Red
B)Yellow
C)Yellow with black band
D)Green

Question 37

In which trash bin must Jay-ar dispose the needles and other sharps?

A)Black
B)Green
C)Red
D)Yellow

Question 38

In which trash bin must Adrian dispose tissues that were fixed with mercuric chloride?

A)Orange
B)Yellow with black band
C)Red
D)Green

Question 39

After the frozen section biopsy, Harry disposes properly the tumor specimen of a patient who previously underwent radiation therapy in which of the following trash bins?

A)Orange
B)Yellow
C)Red
D)Green

Question 40

When carrying and transporting the microscope, what is the proper way of handling the microscope?

A)Hold the arm with the other hand and supporting the base with the other hand
B)Support the base with two hands
C)Hold the arm with both hands
D)Hold the arm with one hand

Question 41

Sheena is correct when she uses a fire extinguisher bearing the fire classification that is made for fires on wooden cabinets and shelves with documents inside it?

A)Fire classification A
B)Fire classification B
C)Fire classification C
D)Fire classification D

Question 42

In which classification does fire brought about by faulty electrical wiring of the fixation machine belong?

A)Fire classification A
B)Fire classification B
C)Fire classification C
D)Fire classification D

Question 43

Which of the following is not a precaution to be observed by Jaz regarding fire extinguishers?

A)Do not incinerate the container even when empty.
B)Do not place on wet places
C)Do not place under direct sunlight
D)Do not store the fire extinguisher in the corner of a room

Question 44

How many micrometers should a histotechnician cut a tissue that is to be cut in 0.5 micra of thickness?

A)0)5 um
B)50 micometers
C)500 mcm
D)0)005 micrometers

Question 45

As a histotechnician, one must know the basic knowledge in handling hazardous histological chemicals. As a rule of the thumb, diluting acids should be done correctly using which of the following procedure?

A)When diluting a concentrated acid, pour water into the acid and this should be done under the chemical fume hood.
B)When diluting a concentrated acid, pour the acid into the water and this should be done under the chemical fume hood.
C)When diluting a concentrated acid, pour the acid into the water and this should be done in the sink.
D)When diluting a concentrated acid, pour the acid into the water. This should be done on a place in the laboratory where nobody is present.