Deck 5: Staining

Question 1

Which of the following stains is a coal tar dye?

A)Orcein
B)Saffron
C)Hematoxylin
D)Aniline dye

Answer 1

Aniline dye

Question 2

This is a natural dye derived through the extraction from the heartwood of a Mexican tree.

A)Orcein
B)Saffron
C)Hematoxylin
D)Cochineal dye

Answer 2

Hematoxylin

Question 3

What is the use of a mordant?

A)To form a bridge between the stain used and the tissue to be stained.
B)To decolorize the stained tissue.
C)To act as a counterstain that will stain the tissue with pinkish to red and the background as blue.
D)To combine aqueous solutions of acid and basic dyes.

Answer 3

To form a bridge between the stain used and the tissue to be stained.

Question 4

What stain is used for the study of spermatogenesis?

A)Alum hematoxylin
B)Copper hematoxylin
C)Iron hematoxylin
D)Ripened hematoxylin

Question 5

Weigert's stain is an example of what stain?

A)Copper hematoxylin
B)Iron hematoxylin
C)Alum hematoxylin
D)Ripened hematoxylin

Question 6

This is a fluorescent red dye commonly used as a background stain particularly in chromate and picric acid fixed tissues and in acid decalcified materials?

A)Hematoxylin
B)Eosin
C)Methylene blue
D)Iodine

Question 7

Eosin is a red acid dye available in three forms. What form of eosin is most commonly used as a stain for highlighting cytoplasmic material in the samples?

A)Eosin Y
B)Eosin B
C)Eosin S
D)None of the Above

Question 8

This natural dye is an excellent stain for elastic fibers and is especially recommended in dermatological studies?

A)Toluidine blue
B)Neutral red
C)Carmine
D)Orcein

Question 9

Histological staining is the process of staining tissue constituents by direct interaction with a dye or staining solution. Listed below are classifications of histological staining, except:

A)Periodic Acid Schiff stain
B)Micro-anatomic stains
C)Bacterial stains
D)Neurologic stains

Question 10

Natural dyes are those obtained from plants and animals. Which of the following dyes does not come from these sources?

A)Hematoxylin
B)Orcein
C)Saffron
D)Aniline

Question 11

Synthetic dyes are sometimes known as:

A)Cochineal dyes
B)Logwood dyes
C)Coal Tar dyes
D)Natural dyes

Question 12

What do you call this method of staining that uses another agent or Mordant to make the staining reaction possible?

A)Direct staining
B)Regressive staining
C)Indirect Staining
D)Progressive staining

Question 13

In the indirect staining method, this non-mordant substance is not essential to the chemical union of tissue and dye and does not participate but only accelerates or hastens the speed of the staining reaction.

A)Accentuator
B)Dye
C)Mordant
D)Accelerator

Question 14

Name the substance that intensifies the action of the dye or stain in cell or tissue preparations.

A)Mordant
B)Accentuator
C)Accelerator
D)Dye

Question 15

Which among the following is a group of a metachromatic dye?

A)Thizine
B)Triphenylmethane
C)Both A and B
D)Neither A nor B

Question 16

What staining method involves the technique of decolorization?

A)Progressive staining
B)Regressive staining
C)Direct staining
D)Indirect staining

Question 17

In the regressive method of staining, this substance acts as a differentiator for both basic and acidic dyes?

A)Alcohol
B)Acetone
C)Xylene
D)Water

Question 18

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?

A)Regressive staining
B)Direct staining
C)Progressive staining
D)Metachromatic staining

Question 19

All of the following are metachromatic dyes, except:

A)Safranin
B)Toluidine blue
C)Bismarck brown
D)Eosin Y

Question 20

Trypan blue vitally stains which specific cell structure?

A)Cytoplasm
B)Mitichondria
C)Nucleus
D)Reticulo-Endothelial System

Question 21

In Counterstaining, all will color the cytoplasm red, except:

A)Eosin Y
B)Neutral Red
C)Eosin B
D)Phloxine B

Question 22

What part of a living cell is considered to be resistant to vital stains?

A)Nucleus
B)Mitochondria
C)RES
D)None of the above

Question 23

All of the following are not supravital stains, except:

A)Lithium
B)Carmine
C)India Ink
D)Thionine

Question 24

Eosin Y is a counterstain that colors:

A)Red
B)Yellow
C)Blue
D)Green

Question 25

Most of these fixatives can be used in Routine H & E staining in Paraffin Embedded Sections, except:

A)Alcohol
B)Osmic Acid
C)Mercuric Chloride
D)Acetone

Question 26

It is an oxazine dye used as an alternative to iron hematoxylin nuclear stain?

A)Celestine blue
B)Trypan blue
C)Nile blue
D)Toluidine blue

Question 27

What group of dyes is insoluble in water but soluble in alcohol?

A)Acid dyes
B)Basic dyes
C)Neutral dyes
D)None of the above

Question 28

What specific type of stain is acid mucin?

A)Progressive stain
B)Regressive stain
C)Metachromatic stain
D)None of the above

Question 29

Of the following choices below, which one is not a type of neutral dye?

A)Romanowsky dyes
B)Giemsa stain
C)Irishman's stain
D)Methylene blue

Question 30

What is the purpose of 10 ml ethyl alcohol in Harris Hematoxylin staining solution?

A)Antibacterial
B)Antifungal
C)Fixative
D)None of the above

Question 31

This is considered as an excellent stain for acid mucopolysaccharides. What is this specific stain?

A)Aniline blue
B)Alcian blue
C)Celestine blue
D)Congo Red

Question 32

The staining done by Janus Green B to demonstrate mitochondria is an example of what type of staining method?

A)Vital staining
B)Counterstaining
C)Direct staining
D)None of the above

Question 33

This staining method is use in Reticulocyte counting. What do you call this method?

A)Supravital staining
B)Direct staining
C)Intravital staining
D)None of the above

Question 34

Which of the following statements is not true about Mayer's Hematoxylin?

A)It is an alum hematoxylin.
B)It is used as a nuclear counterstain.
C)It is used only for regressive staining.
D)It is chemically ripened with sodium iodate.

Question 35

What staining solution does not belong to the group?

A)Ehrlich's hematoxylin
B)Harris's hematoxylin
C)Cole's hematoxylin
D)Heidenhain's hematoxylin

Question 36

Iron hematoxylin is a ferric salt used to ripen hematoxylin. Which of the following statements is not correct about iron hematoxylin?

A)It is used only for regressive staining.
B)It uses acid-alcohol as a differentiating agent.
C)Tissues structures are stained blackish or grayish.
D)The two main iron hematoxylin solutions are Weigert's and Cole's hematoxylin solution.

Question 37

Eosin is a red dye available in how many forms?

A)1
B)2
C)3
D)4

Question 38

Among the oil soluble dyes, which dye is considered to be the most sensitive in staining intracellular fats?

A)Sudan Black B
B)Sudan III
C)Sudan IV
D)None of the above

Question 39

This dye is an essential component of the Schiff reagent used in histochemical staining of carbohydrates?

A)Acid fuchsin
B)Basic fuchsin
C)Carmine
D)Aniline blue

Question 40

In glycogen demonstration, what stain is widely used?

A)Methylene blue
B)Aniline blue
C)Carmine
D)Periodic Acid Schiff

Question 41

Which among the following methods can the Schiff reagent be possibly prepared?

A)Barger and Delamater
B)De Tomasi Coleman
C)Hikawa and Oguru
D)All of the above

Question 42

In preparing the Schiff reagent through the Barger and Delamater method, what solution is added to the Schiff reagent?

A)Thionyl chloride
B)Sulfur dioxide gas
C)Sodium potassium metabisulfite
D)None of the above

Question 43

What stain is used as a counterstain in Best Carmine Method?

A)Cole's hematoxylin
B)Harris' hematoxylin
C)Ehrlich's hematoxylin
D)Mayer's hematoxylin

Question 44

In H & E staining, mucins will produce what color when stained with eosin?

A)Light pink color
B)Light blue
C)Red
D)Yellow

Question 45

Below are examples of oil soluble dyes, which dye is used to stain phospholipids as well as neutral fats?

A)Sudan IV
B)Sudan III
C)Sudan Red VII B
D)Sudan Black B

Question 46

What fat stain was popularized by Lilie and Ashburn in 1943?

A)Sudan III
B)Sudan Black B
C)Sudan IV
D)Oil Red O

Question 47

This fluorescent stain is used to demonstrate acid mucins. What do you call this stain?

A)Methylene blue
B)Acridine orange
C)Alcian blue
D)Rhodamine B

Question 48

The components of this dye are capable of differentiating two lipid classes. What is this dye?

A)Methylene blue
B)Nile blue sulfate
C)Osmic acid
D)Aniline blue

Question 49

It is an acid dye that stains basic protamines and histones?

A)Fast green
B)Nile blue
C)Prussian blue
D)Methyl green

Question 50

Which histochemical staining technique used for DNA is the most reliable and specific?

A)Feulgen's reaction
B)Methyl-green pyronin
C)Both
D)Neither

Question 51

The most commonly used fluorochrome to demonstrate DNA and RNA in fresh or fixed tissues is?

A)Janus green B
B)Acridine orange
C)Victoria blue
D)Night blue

Question 52

In the Feulgen technique of acid hydrolysis, how many percent of alcoholic solution of Acriflavine is needed in order to be used as an alternative to basic fuchsin in Schiff's reagent?

A)0)01 %
B)0)02 %
C)0)03 %
D)0)04 %

Question 53

Below are techniques used in staining collagen. Which of the following techniques is not used for such purpose?

A)Van Gieson's stain
B)Masson's Trichome stain
C)Malloy's Aniline blue stain
D)Taenzer-Unna Orcein method

Question 54

Which staining method of collagen is considered the simplest method of differential staining?

A)Van Gieson's stain
B)Masson Trichome stain
C)Mallory's aniline blue stain
D)Azocarmine stain

Question 55

The following choices are the common staining methods used for elastic fibers, except:

A)Taenzer-Unna Orcein method
B)Verhoeff's stain
C)Krajian's method
D)Azocarmine stain

Question 56

In Masson's Trichome staining collagen, what are the fixatives used for fixation?

A)Zenker
B)Helly
C)Bouin's
D)All of the above

Question 57

What will be the color of Elastic fibers when stained with Weigert's?

A)Dark blue
B)Blue black
C)Gray
D)Both A and B

Question 58

In high pH Congo red technique for amyloid, the solutions used are buffered to what pH?

A)7
B)8
C)9
D)10

Question 59

Bielschowsky's Technique is used for staining what part of the Central Nervous System?

A)Neurons
B)Axons
C)Neurofibrils
D)All of the above

Question 60

All of the following substances make up the Romanowsky stains, except:

A)Methylene blue
B)Azure B
C)Eosin
D)Hematoxylin

Question 61

What technique is useful in staining encapsulated fungi?

A)Southgates's mucicarmine
B)Best carmine
C)Langhan's iodine
D)Gomori's aldehyde

Question 62

In this technique, ferricyanide is reduced to ferrocyanide. When in the presence of ferric salts, Prussian blue will be produced. What do you call this method?

A)Schmorl's Ferric Ferricyanide
B)Gomori's Aldehyde
C)Gmelin Technique
D)Modified Fouchet's technique

Question 63

Melanin is best demonstrated by what staining technique?

A)Von Kossa's silver nitrate method
B)Masson Fontana silver technique
C)Lindquist's Modified Rhodanine technique
D)None of the above

Question 64

Lindquist's Modified Rhodanine Technique is used for staining Copper. In this technique, what medium is preferred for mounting?

A)Farrant's medium
B)Brun's fluid
C)Apathy's medium
D)Glycerin

Question 65

What method of staining is used for Acid-Fast Bacteria?

A)Ziehl Neelsen
B)Brown and Brenn
C)Wade-Fite technique
D)Gram's stain

Question 66

Which among the following is the staining method that involves the staining of living cells through the injection of the dye?

A)Vital staining
B)Intravital staining
C)Supravital staining
D)Metachromatic staining

Question 67

What is the active coloring agent of hematoxylin?

A)Hematin
B)Orcein
C)Aniline
D)None of the above

Question 68

The process of ripening can be accelerated through these oxidizing agents. What are these agents?

A)Hydrogen peroxide
B)Mercuric oxide
C)Potassium permanganate
D)All of the above

Question 69

What substance is responsible for the coloring property of a dye?

A)Auxochrome
B)Chromophore
C)Both A and B
D)None of the above

Question 70

Best Carmine solution is a combination of Carmine and what substance?

A)Picric Acid
B)Ammonia
C)Aluminum Chloride
D)Mercuric chloride

Question 71

What is the stain used for metallic impregnation?

A)Bismarck brown
B)Celestine blue
C)Gold sublimate
D)Congo red

Question 72

This is the oldest stain and was originally used for microscopic study of starch granules?

A)Methylene blue
B)Eosin
C)Iodine
D)Safranin

Question 73

It is a valuable stain for plasma cells? What is this stain?

A)Methylene blue
B)Aniline blue
C)Methyl green
D)Neutral red

Question 74

Which of the following choices is a metachromatic dye?

A)Iodine
B)Malachite green
C)Methyl green
D)Methylene violet

Question 75

What is the stain recommended for staining Nissl granules or chromophilic bodies?

A)Toluidine blue
B)Victoria blue
C)Methylene blue
D)Night blue

Question 76

This oil soluble dye has no secondary amino group, making it hard to stain phospholipids. What is this dye?

A)Sudan Black
B)Sudan III
C)Sudan IV
D)Oil Red O

Question 77

What fat stain is good for staining Central Nervous System samples?

A)Sudan Black B
B)Sudan III
C)Sudan IV
D)Oil Red O

Question 78

The development of a reddish purple color when few drops of the Schiff reagent are added to 10 mL of 37-40% formaldehyde indicates which of the following?

A)Schiff reagent is still good and usable
B)Schiff reagent is not effective
C)Schiff reagent should be discarded
D)None of the above

Question 79

It is a histological dye used for the demonstration of acid mucins?

A)Alcian blue
B)Night blue
C)Methylene blue
D)Victoria blue

Question 80

What do you call this stain that is associated with Tay-Sach's disease?

A)Modified PAS
B)Nile blue
C)Toluidine blue
D)Borohydride-periodic-schiff