Deck 5: Gel Electrophoresis Techniques and Applications

Question 1

Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?

A)Circular
B)Supercoiled
C)Nicked
D)Supercoiled and circular will move at same speed and faster than nicked

Answer 1

Supercoiled

Question 2

How is the size of molecules under analysis measured?

A)By measuring the distance moved through a ruler
B)By measuring the amount of visualising dye used
C)By running a standard molecule, whose size is known in parallel
D)There is no exact criterion for doing so

Answer 2

By running a standard molecule, whose size is known in parallel

Question 3

Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels.

A)Agarose is a polysaccharide which is obtained from red algae
B)Agarose is a polysaccharide obtained from fungus
C)It is composed of glucose residues
D)It is obtained via dissolving in its water by boiling in water and then cooling it

Answer 3

Agarose is a polysaccharide which is obtained from red algae

Question 4

Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

A)They are obtained via polymerization between acrylamide and bis-acrylamide
B)The components added for initiating polymerization are ammonium persulphate and TEMED
C)It is casted in horizontal and flat trays
D)TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

Question 5

If the amount of agarose added is more, the molecular under analysis should have the following characteristic………………..

A)small size
B)large size
C)size has no relation with the amount of agarose
D)the amount of molecules under analysis matters

Question 6

In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?

A)Renaturing
B)Denaturing
C)The routine agarose gel
D)The routine polyacrylamide gel

Question 7

The technique used to located specific genes in chromosomes is ……………..

A)Colony Hybridization
B)In situ Hybridization
C)Dot Blot Hybridization
D)Western Blotting

Question 8

The separation of charged molecules under the influence of electric current is called……….

A)Colony Hybridization
B)Dot Blot Hybridization
C)Electrophoresis
D)Western Blotting

Question 9

If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________

A)secondary electrophoresis
B)recovery electrophoresis
C)denaturing electrophoresis
D)electro-elution

Question 10

The greatest separation is obtained in which portion of the gel?

A)Lower portion where the anode is
B)Lower portion where the cathode is
C)The separation is uniform all over
D)It varies according to the quantity of the size of the molecules to be separated