Deck 9: Proteins

Question 1

The common ingredients of all biuret reagents are:

A) Sodium potassium tartrate, copper sulfate, and sodium hydroxide.
B) Sodium tungstate and sulfuric acid.
C) Copper sulfate and NaOH.
D) Ammonium sulfate and sodium hydroxide.

Answer 1

Sodium potassium tartrate, copper sulfate, and sodium hydroxide.

Question 2

Hyperalbuminemia is caused by:

A) Dehydration syndromes.
B) Liver disease.
C) Burns.
D) Gastroenteropathy.

Answer 2

Dehydration syndromes.

Question 3

Bromcresol Green (BCG) may be used in the dye-binding assay of:

A) Lipoprotein.
B) Cholesterol.
C) Albumin.
D) 17-ketosteroids.
E) Estrogen.

Answer 3

Albumin.

Question 4

In agarose gel electrophoresis of human serum at pH 8.6, the slowest moving fraction is:

A) ?-1 globulin.
B) ?-globulin.
C) ?-2 globulin.
D) Albumin.
E) ?-globulin.

Question 5

High serum total protein but low albumin is usually seen in:

A) Multiple myeloma.
B) Hepatic cirrhosis.
C) Glomerulonephritis.
D) Nephrotic syndrome.

Question 6

Which of the following serum proteins migrates with the beta globulins on cellulose acetate at pH 8.6?

A) Ceruloplasmin
B) Hemopexin
C) Haptoglobin
D) Transthyretin

Question 7

Absence of a large decrease in the ?-1 globulin peak in a serum electrophoresis pattern suggests:

A) Transferrin deficiency.
B) ?_1- antitrypsin deficiency.
C) Nephrotic syndrome.
D) Chronic inflammation.

Question 8

A protein that is increased or normal in nephrotic syndrome is:

A) ?₁-antitrypsin.
B) ?₂-macroglobulin.
C) Albumin.
D) Hemopexin.

Question 9

The principal protein for transport of iron from the intestine to the liver, bone marrow, or spleen for storage is:

A) Haptoglobin.
B) Transferrin.
C) Ceruloplasmin.
D) Alpha-fetoprotein.

Question 10

Ceruloplasmin is an ?₂-globulin that contains:

A) Copper.
B) Manganese.
C) Iron.
D) Zinc.

Question 11

Myeloma (Bence Jones) protein is MOST often found electrophoretically in the area of:

A) Albumin or ?₁-globulins.
B) ?₂-globulins.
C) ? or ? globulins.
D) ?₁- or ?₂-globulins.
E) ?-globulins.

Question 12

A decrease in this protein will result in an emphysema-like condition.

A) ?₁-antitrypsin
B) ?₂-macroglobulin
C) ?-lipoprotein
D) Hemopexin

Question 13

In an Acute Phase Reaction (M.I., etc.), there is a(n):

A) Decrease in albumin, increase in ?₁-globulin, ?₂-globulin, and ?₃-globulin.
B) Intense band in ?₁-globulin, increase in albumin.
C) Marked decrease in ?-globulin.
D) Marked increase in immunoglobulin A.
E) Increase in ?₂-globulin.

Question 14

Which of the following conditions is usually associated with an acute inflammatory pattern?

A) Myocardial infarction
B) Rheumatoid arthritis
C) Hepatitis
D) Surgery

Question 15

Mary's total protein is 7.5 g/dL and her albumin is 4.2 g/dL. What is her A/G ratio?

A) 1.1
B) 1.3
C) 1.5
D) 1.8

Question 16

Which protein band comprises 2-5% of the relative protein?

A) Albumin
B) ?₁-globulin
C) ?₂-globulin
D) ?-globulin

Question 17

What is the clinical significance of testing for Transthyretin?

A) High levels are associated with an acute phase reaction.
B) Levels are associated with glomerular injury in diabetic nephropathy.
C) Low levels indicate compromised nutritional status.
D) Low levels are associated with increased free cortisol.

Question 18

Bill has a total protein of 7.5 g/dL and his protein electrophoresis reports a 12% ?-globulin. What is his ?-globulin level in g/dL?

A) 0.6 g/L
B) 0.9 g/L
C) 1.2 g/L
D) 1.5 g/L

Question 19

Which condition is associated with fusion of the ?-? bridging?

A) Cirrhosis
B) Acute pancreatitis
C) Myocardial infarction
D) Nephrotic syndrome

Question 20

Which of the following conditions is associated with an elevated total protein?

A) Cirrhosis
B) Glomerular disease
C) Starvation
D) Malignancy

Question 21

Which of the following proteins is part of the coagulation cascade?

A) Complement
B) Fibrinogen
C) Transferrin
D) ?-microglobulin