Deck 25: Mycoplasma and Ureaplasma

Question 1

What test method is used to detect antibodies to Mycoplasma spp.?

A)Complement fixation (CF)
B)Enzyme immunoassay (EIA)
C)Direct fluorescent antibody (DFA)
D)Cellulose ion exchange (CIE)

Answer 1

B
Until recently, the most commonly used technique for demonstration of Mycoplasma pneumoniae antibodies was the micromethod CF assay, which was time-consuming and had inherent technical problems.EIA and micro-IF are now available for the detection of serum antibodies.

Question 2

What two organisms are associated with urogenital tract infections but have also been isolated from asymptomatic individuals?

A)Mycoplasma hominis and Ureaplasma urealyticum
B)M.hominis and M.fermentans
C)U.urealyticum and M.pneumoniae
D)M.penetrans and M.pneumoniae

Answer 2

A
Both M.hominis and U.urealyticum are associated with infections of the urogenital tract.They are, however, frequently isolated from asymptomatic individuals, making interpretation of a positive culture difficult.

Question 3

All the following antibiotics are used to treat mycoplasma infections except:

A)macrolides.
B)fluoroquinolones.
C)tetracyclines.
D)penicillin.

Answer 3

D
Because they lack a cell wall, the mycoplasmas are inherently resistant to the b-lactams: penicillins and cephalosporins.Mycoplasma pneumoniae has remained susceptible to the tetracyclines, newer fluoroquinolones, and the macrolides.

Question 4

Mycoplasmas are a different type of bacteria because they do not have a:

A)cell wall.
B)cell membrane.
C)nuclear membrane.
D)messenger transporter.

Question 5

One of the factors that allows Mycoplasma to infect the respiratory tract and the urogenital tract is that:

A)the body has a hard time detecting their presence, because they have no cell wall.
B)they are virulent organisms.
C)they adhere to the epithelium of mucosal surfaces and are not eliminated by mucus secretions.
D)all of the above.

Question 6

Which factor is added to media to provide cholesterol for mycoplasma-like organisms?

A)Trypticase soy digest
B)20% fetal calf serum
C)X and V factor supplement
D)5% sheep blood

Question 7

What organism has been recovered in normal, sexually active females but is also associated with chorioamnionitis, congenital pneumonia, and development of chronic lung disease in premature infants?

A)Mycoplasma hominis
B)Ureaplasma ureolyticus
C)M.penetrans
D)M.fermentans

Question 8

What disease causes the condition primary atypical pneumonia?

A)Mycoplasma hominis
B)M.urealyticum
C)M.fermentans
D)M.pneumoniae

Question 9

Mycoplasma organisms will grow in which of the following atmospheres?

A)Ambient
B)Capnophilic
C)Anaerobic
D)All of the above

Question 10

Cultures for Mycoplasma pneumoniae should be incubated for:

A)2 to 3 days.
B)5 to 7 days.
C)10 to 14 days.
D)21 days or more.

Question 11

Which antibiotic is added to mycoplasma isolation and transport media to minimize bacterial contamination?

A)Tetracycline
B)Penicillin
C)Erythromycin
D)Gentamicin

Question 12

When transporting specimens for Mycoplasma culture to the laboratory, extreme care must be taken so that the specimen does not:

A)become contaminated.
B)leak from the specimen container.
C)become overgrown with bacterial normal flora.
D)dry out.

Question 13

Which of the following methods is used to determine the presence of growth in mycoplasma broth media?

A)Broth turbidity
B)Presence of biofilm on surface
C)Change in phenol red pH indicator
D)Release of radiolabeled CO2

Question 14

When Mycoplasma hominis invades the upper genitourinary tract, it can cause:

A)salpingitis.
B)pyelonephritis.
C)pelvic inflammatory disease.
D)all of the above.

Question 15

What time frame is suitable for blood draws for serologic testing to diagnose Mycoplasma infections?

A)Onset of symptoms and 2 to 3 weeks later
B)Week after symptoms start and 2 months later
C)Weeks after symptoms start and 3 months later
D)Onset of symptoms and 2 months later

Question 16

At-risk groups for contracting walking pneumonia include all the following except:

A)prisoners.
B)college students.
C)military personnel.
D)preschool children.

Question 17

If a newborn has symptoms of meningitis but the cerebrospinal fluid (CSF) is negative for both Gram stain and a routine culture, what organisms should be suspected?

A)Neisseria gonorrhea and Chlamydia trachomatis
B)Treponema pallidum and Borrelia burgdorferi
C)Mycoplasma hominis and Ureaplasma urealyticum
D)M.pneumoniae and C.trachomatis

Question 18

Which of the following stains is used to enhance the visibility of mycoplasma-like colonies?

A)Gram stain
B)Acridine orange
C)Dienes stain
D)Wright's stain

Question 19

Mycoplasma spp.from patient samples are visible by which staining technique?

A)Gram stain
B)Wright's stain
C)Acridine orange
D)All of the above

Question 20

Which of the following organisms was once called "T-strain mycoplasma?"

A)Ureaplasma spp.
B)Mycoplasma hominis
C)Mycoplasma fermentans
D)Mycoplasma orale