Deck 76: Normally Sterile Body Fluids, Bone and Bone Marrow, and Solid Tissues

Question 1

Which fungal condition is most often detected in the bone marrow?

A) Brucellosis
B) Histoplasmosis
C) Tuberculosis
D) Leishmaniasis

Answer 1

B
The diagnosis of diseases, including brucellosis, histoplasmosis, blastomycosis, tuberculosis, and leishmaniasis, can sometimes be made by the detection of organisms in the bone marrow; however, only histoplasmosis is a fungus.

Question 2

Pericardial fluid may be inoculated in blood culture broth and processed as a blood culture, provided the broth contains which anticoagulant?

A) Heparin
B) Ethylenediaminetetraacetic acid (EDTA)
C) Citrate
D) Coumadin

Answer 2

C
With respect to pericardial, pleural, synovial, and peritoneal fluids, the inoculation of blood culture broth bottles at the bedside or in the laboratory may be beneficial.An additional specimen should be submitted to the laboratory for a Gram stain.The specimen in the blood culture bottle is processed as a blood culture, facilitating the recovery of small numbers of organisms and diluting out the effects of antibiotics.Citrate or sodium polyanethol sulfonate (SPS)may be used as an anticoagulant.

Question 3

Fluids should be processed for direct microscopic examination.If one organism is observed per oil immersion field, then at least _____ organisms per milliliter of specimen are present.

A) 102
B) 103
C) 104
D) 105

Answer 3

D
All fluids should be processed for direct microscopic examination.In general, if one organism is observed per oil immersion field, then at least 10⁵ organisms per milliliter of specimen are present.

Question 4

The most common etiologic agent of septic arthritis accounting for approximately 70% of infections is:

A) Streptococcus pyogenes.
B) Haemophilus influenza.
C) Staphylococcus aureus.
D) Streptococcus mitis.

Question 5

Any tissues received in the clinical microbiology laboratory should be minced and homogenized in a biosafety cabinet.If lung tissue is sent and processed, then which organism should be examined using direct fluorescent antibody (DFA)testing?

A) Listeria
B) Legionella
C) Leishmania
D) Leptospira

Question 6

Most cases of peritonitis in patients on continuous ambulatory peritoneal dialysis (CAPD)are caused by:

A) Staphylococcus epidermidis and S. aureus.
B) Escherichia coli.
C) Klebsiella pneumoniae.
D) Streptococcus spp.
E) viral agents.

Question 7

The most common etiologic agent of septic arthritis in adults younger than 30 years of age is:

A) Neisseria gonorrhoeae.
B) Staphylococcus aureus.
C) Haemophilus influenzae.
D) Streptococcus agalactiae.

Question 8

The proper technique for processing clotted body fluids sent to the laboratory for fungal culture is:

A) grinding them in a mortar and pestle or glass tissue grinder.
B) placing small amounts of whole material from the clot directly onto media.
C) centrifugation with glass beads.
D) filtration under pressure.

Question 9

A 68-year-old man has an osteomyelitis infection as a result of bacteremia.The most common etiologic agent found in this infection is:

A) Haemophilus spp.
B) Staphylococcus aureus.
C) Pseudomonas aeruginosa.
D) Fusobacterium necrophorum.

Question 10

Human bites and animal bites caused by ________ and _________, respectively, can ultimately lead to an underlying bone infection.

A) Eikenella corrodens; Actinomyces spp.
B) Pasteurella multocida; Peptostreptococcus spp.
C) Prevotella spp.; Staphylococcus aureus
D) Eikenella corrodens; Pasteurella multocida