Deck 5: The Microscope

Question 1

The magnifying powers of the usual microscope objectives are:

A) 5×, 10×, 20×.
B) 10×, 20×, 40×.
C) 10×, 40×, 80×.
D) 10×, 40×, 100×.

Answer 1

10×, 40×, 100×.

Question 2

Oil needs to be removed from the:

A) ocular.
B) 10× objective.
C) 40× objective.
D) 100× objective.

Answer 2

100× objective.

Question 3

The phase-contrast microscope differs from the brightfield microscope because of changes in:

A) the objective.
B) the condenser.
C) the oculars.
D) both a and b.

Answer 3

both a and b.

Question 4

In a polarizing microscope,the polarizing filters are:

A) placed on top of each other.
B) placed at right angles to each other.
C) red and blue in color.
D) used to brighten the field being observed.

Question 5

The definition of Köhler illumination is the:

A) strength of the transmitted light.
B) light path through the microscope.
C) light path passing through the condenser of the microscope.
D) light path passing through the diaphragm of the microscope.

Question 6

The most common type of microscope illumination system used in the clinical laboratory is:

A) brightfield.
B) phase contrast.
C) polarizing.
D) fluorescent.

Question 7

Fluorescent objects (natural,stained,or labeled)can be seen with a fluorescent microscope because they absorb the ____ light and emit light of yellow or ____ wavelength.

A) blue; green
B) red; orange
C) green; purple
D) purple; red

Question 8

Total magnification is the product of the magnification of the objective ____ the magnification of the ocular.

A) added to
B) multiplied by
C) divided by
D) subtracted from

Question 9

The term ocular is another name for the microscopic ____ that magnifies an image.

A) objective
B) eyepiece
C) diaphragm
D) nosepiece

Question 10

The definition of the microscopic working distance is the distance from the:

A) bottom of the objective to the material being studied.
B) ocular to the bottom of the objective.
C) ocular to the material being studied.
D) top of the objective to the material being studied.

Question 11

An artificial neural network:

A) simulates the action of a robot.
B) simulates human brain processing of information.
C) simulates automated instruments.
D) follows strict algorithms.

Question 12

The definition of microscopic resolution is:

A) an indication of how small individual dots can be and still be recognizable.
B) an indication of how close individual objects (dots) can be and still be recognizable.
C) the point at which the appearance of two dots results in a dumbbell appearance.
D) both a and b.

Question 13

The numerical aperture (NA)is:

A) an index of the resolving power of a lens.
B) the distance from an object being viewed to the lens.
C) inscribed on each objective lens.
D) both a and b.

Question 14

The most dramatic change in microscopy in the last 3 decades,has been:

A) invention of the electron microscope.
B) the ability to digitize images.
C) the ability to transmit microscopic images electronically.
D) both b and c.

Question 15

A brightfield microscope may be converted to a darkfield microscope by:

A) use of a special darkfield condenser in place of the usual condenser.
B) replacement of the usual filters.
C) use of a special ocular.
D) changing the type of light source.