Deck 17: Manipulating the Genomes of Eukaryotes

Question 1

What is the purpose of the P element in the creation of trasngenic Drosophila?

A)Efficiently transfer DNA into Drosophila chromosomes
B)Transfer DNA into a specific site in the host chromosome.
C)Transfer DNA only into the nuclei of somatic cells.
D)Transfer DNA only into primitive eye cells.

Answer 1

Efficiently transfer DNA into Drosophila chromosomes

Question 2

TALENs are bioengineered molecules that include an endonuclease peptide and a

A)DNA binding domain created from motifs of a bacterial protien
B)DNA binding domain from a eukaryotic transcription factor.
C)DNA binding domain from the Lac repressor.
D)DNA binding domain of a DNA polymerase.

Answer 2

DNA binding domain created from motifs of a bacterial protien

Question 3

Transgenic animals can be limited in terms of their study of the links between diseases and specific genes since

A)the transgene must be dominant and some diseases are caused by recessive mutations.
B)the transgene must be recessive and some diseases are caused by dominant mutations.
C)all disease are the cause of mutations in multiple genes..
D)diseases are not caused by the expression of a gene but on post-translational modifications of the protein products.

Answer 3

the transgene must be dominant and some diseases are caused by recessive mutations.

Question 4

Why are conditional knockout animals desirable?

A)genes that are essential for development can be deleted in the adult.
B)animal cell lines with deletions can be created.
C)they are easier to create than standard knockout animals.
D)the mutations they create are more stable than those created by standard knockout techniques.

Question 5

How can transgenics be used in gene expression studies?

A)A reporter gene can be cloned so that it is under the control of tissue specific regulatory regions.
B)A gene from the trasngenic chromosome can be cloned under an arteficial promoter and enhancer regions.
C)A gene from the trasngenic chromosomes can be cloned under the control of its normal regulatory regions.
D)A misense mutation of a gene froound in the transgenic organisms genome can be cloned under the control of synthetic regulatory regions.

Question 6

What is one problem associated with using retroviruses as human gene therapy vectors?

A)Their random integration into the host chromosome may result in detrimental mutations.
B)They need to integrate through an RNA intermediate.
C)The level of proteins produced from them is usually very low.
D)The level of proteins produced from them is usually very high.

Question 7

The Ti plasmid allows for

A)the transfer of the plasmid from Agrobacterium to a plant cell.
B)trasnfer of plant DNA to Agrobacterium.
C)the trasnfer of DNA between two Agrobaterium cells.
D)the transfer of the plasmid from Agrobacterium to
E)coli.

Question 8

A unique trait of embryonic stem cells that make them useful for generating knockout animals is that they

A)are totipotent.
B)can be grown in culture.
C)have been isolated from mice.
D)are extremely well characterized at the molecular level.

Question 9

Transgenic organisms can help clarify which gene may be responsible for a particular phenotype since

A)individuals with the phenotype frequently carry other mutations as well.
B)the genes that cause disease are always dominant.
C)the genes that cause diseases are always recessive.
D)strains used to create transgenic organisms carry no mutatins all their genes are wild-type.

Question 10

Knockin mice can be created using the Cre-lox system.In this procedure the mutation to be knockedin is

A)cloned outside of the two lox sites.
B)cloned between the two lox sites.
C)can be cloned either between or outside of the two lox sites.
D)cloned on the plasmid that expresses the the Cre protein.

Question 11

Currently the two main vectors for delivery of therapeutic genes for human gene therapy are

A)AAV and retroviruses
B)AAV and Agrobacterium
C)Retroviruses and Agrobacterium
D)AAV, retroviruses and Agrobacterium

Question 12

To create transgenic mice DNA is injected into

A)a pronucleus of a zygote
B)the cytoplasm of a zygote
C)the nucleus after the pronuclei have fused.
D)a pronucleus of one cell from an embryo.

Question 13

What is a possible disadvantage of using genetically modified crops?

A)The trasngene may make its way into non-transgenic plants.
B)Transgenic strains are typically less competetive than the usual strains used for farming.
C)The ability of transgenic plants to use nutrients is reduced so more fertilizer has to be applied.
D)The ability of transgenic strains to use water is not as good so these strains are not drought tolerant.

Question 14

Although bacteria can be used for the production of proteins useful for therapeutic uses what can be a disadvantage to using a prokaryotic system?

A)Proteins may not be post-translationally prcoesssed correctly
B)Transcripts from eukaryotic open reading frames are degraded quickly in prokaryotic cells.
C)Some prokaryotic promoters do not work well to transcribe eukaryotic open reading frames.
D)The structures that seukaryotic mRNAs fold into are frequently used as rho termination signals.