Deck 8: An Introduction to Microbial Metabolism: the Chemical Crossroads of Life
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Deck 8: An Introduction to Microbial Metabolism: the Chemical Crossroads of Life
1
Enzymes that catalyze removal of carbon dioxide from a substrate are called
A)phosphotransferases.
B)oxidoreductases.
C)decarboxylases.
D)aminotransferases.
E)ligases.
A)phosphotransferases.
B)oxidoreductases.
C)decarboxylases.
D)aminotransferases.
E)ligases.
C
2
Which of these enzymes is NOT an exoenzyme?
A)ATP synthase
B)streptokinase
C)penicillinase
D)collagenase
E)elastase
A)ATP synthase
B)streptokinase
C)penicillinase
D)collagenase
E)elastase
A
3
Each of the following are true of enzymes except
A)they can be used over and over.
B)they may or may not require cofactors.
C)their active site is specific to the substrate.
D)they increase the activation energy of a reaction.
E)All of the choices are true of enzymes.
A)they can be used over and over.
B)they may or may not require cofactors.
C)their active site is specific to the substrate.
D)they increase the activation energy of a reaction.
E)All of the choices are true of enzymes.
D
4
An apoenzyme
A)is part of a simple enzyme.
B)is also called a coenzyme.
C)is the protein part of a holoenzyme.
D)is often an inorganic metal ion.
E)is an RNA molecule.
A)is part of a simple enzyme.
B)is also called a coenzyme.
C)is the protein part of a holoenzyme.
D)is often an inorganic metal ion.
E)is an RNA molecule.
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5
Enzymes that catalyze moving a phosphate from one substrate to another are called
A)phosphotransferases.
B)oxidoreductases.
C)decarboxylases.
D)aminotransferases.
E)ligases.
A)phosphotransferases.
B)oxidoreductases.
C)decarboxylases.
D)aminotransferases.
E)ligases.
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6
A type of cofactor would be
A)vitamins.
B)metallic ions.
C)active sites.
D)substrates.
E)ribozymes.
A)vitamins.
B)metallic ions.
C)active sites.
D)substrates.
E)ribozymes.
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7
Enzymes are
A)broken down in reactions that require energy input.
B)proteins that function as catalysts.
C)used up in chemical reactions.
D)not needed for catabolic reactions.
E)All of these choices are correct.
A)broken down in reactions that require energy input.
B)proteins that function as catalysts.
C)used up in chemical reactions.
D)not needed for catabolic reactions.
E)All of these choices are correct.
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8
Enzymes that are secreted by a cell to catalyze hydrolysis reactions are
A)apoenzymes.
B)exoenzymes.
C)constitutive enzymes.
D)regulated enzymes.
E)endoenzymes.
A)apoenzymes.
B)exoenzymes.
C)constitutive enzymes.
D)regulated enzymes.
E)endoenzymes.
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9
Formation of peptide bonds between amino acids to build a polypeptide would be called
A)anabolism.
B)phosphorylation.
C)fermentation.
D)exergonic.
E)glycolysis.
A)anabolism.
B)phosphorylation.
C)fermentation.
D)exergonic.
E)glycolysis.
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10
Important components of coenzymes are
A)vitamins.
B)metallic ions.
C)active sites.
D)substrates.
E)ribozymes.
A)vitamins.
B)metallic ions.
C)active sites.
D)substrates.
E)ribozymes.
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11
What is the final electron acceptor during aerobic respiration?
A)pyruvic acid
B)oxygen
C)nitrate
D)Cytochrome c
E)FAD
A)pyruvic acid
B)oxygen
C)nitrate
D)Cytochrome c
E)FAD
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12
_____ reactions are anabolic reactions involving ligases for synthesis and the release of one water molecule for each bond formed.
A)Reduction
B)Condensation
C)Oxidation
D)Transfer
A)Reduction
B)Condensation
C)Oxidation
D)Transfer
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13
Which term refers to all of the chemical reactions of the cell?
A)catabolism
B)redox reactions
C)phosphorylation
D)metabolism
E)cellular respiration
A)catabolism
B)redox reactions
C)phosphorylation
D)metabolism
E)cellular respiration
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14
Ribozymes are
A)ribosomes which catalyze reactions.
B)unique to prokaryotes.
C)unique to eukaryotes.
D)catalysts for RNA editing.
E)catalysts for DNA splicing.
A)ribosomes which catalyze reactions.
B)unique to prokaryotes.
C)unique to eukaryotes.
D)catalysts for RNA editing.
E)catalysts for DNA splicing.
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15
The cell's metabolic reactions involve the participation of _____ that lower the activation energy needed for the initiation of a reaction.
A)cofactors
B)vitamins
C)enzymes
D)ATP
E)Coenzymes
A)cofactors
B)vitamins
C)enzymes
D)ATP
E)Coenzymes
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16
The most likely place where an exoenzyme participates in a chemical reaction is
A)mitochondria.
B)within the cell membrane.
C)lysosomes.
D)cytoplasm.
E)outside of the cell.
A)mitochondria.
B)within the cell membrane.
C)lysosomes.
D)cytoplasm.
E)outside of the cell.
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17
Enzymes that catalyze removing electrons from one substrate and adding electrons to another are called
A)phosphotransferases.
B)oxidoreductases.
C)decarboxylases.
D)aminotransferases.
E)ligases.
A)phosphotransferases.
B)oxidoreductases.
C)decarboxylases.
D)aminotransferases.
E)ligases.
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18
Enzymes that are only produced when substrate is present are termed
A)exoenzymes.
B)endoenzymes.
C)constitutive enzymes.
D)induced enzymes.
E)conjugated enzymes.
A)exoenzymes.
B)endoenzymes.
C)constitutive enzymes.
D)induced enzymes.
E)conjugated enzymes.
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19
A holoenzyme is a combination of a protein and one or more substances called
A)substrates.
B)apoenzymes.
C)catalysts.
D)cofactors.
A)substrates.
B)apoenzymes.
C)catalysts.
D)cofactors.
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20
Reactants are converted to products by
A)enzymes releasing energy.
B)breaking and forming bonds.
C)enzymes binding to reactants.
D)reactants releasing energy.
A)enzymes releasing energy.
B)breaking and forming bonds.
C)enzymes binding to reactants.
D)reactants releasing energy.
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21
Each of the following are electron carriers except
A)NAD.
B)FAD.
C)NADP.
D)FADP.
E)Coenzyme A.
A)NAD.
B)FAD.
C)NADP.
D)FADP.
E)Coenzyme A.
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22
When enzyme action stops due to a buildup of end product, this control is called
A)competitive inhibition.
B)enzyme induction.
C)enzyme repression.
D)feedback inhibition.
A)competitive inhibition.
B)enzyme induction.
C)enzyme repression.
D)feedback inhibition.
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23
Feedback inhibition is best described as
A)substrate binding to DNA, blocking enzyme transcription.
B)product binding to DNA, blocking enzyme transcription.
C)substrate binding to enzyme in noncompetitive site.
D)product binding to enzyme in noncompetitive site.
A)substrate binding to DNA, blocking enzyme transcription.
B)product binding to DNA, blocking enzyme transcription.
C)substrate binding to enzyme in noncompetitive site.
D)product binding to enzyme in noncompetitive site.
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24
Most electron carriers are
A)coenzymes.
B)enzymes.
C)hydrogens.
D)inorganic phosphate.
E)All of these choices are correct.
A)coenzymes.
B)enzymes.
C)hydrogens.
D)inorganic phosphate.
E)All of these choices are correct.
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25
In addition to electrons, which of the following is also involved in electron transfer?
A)ADP
B)glucose
C)carbon
D)hydrogen
E)carbon dioxide
A)ADP
B)glucose
C)carbon
D)hydrogen
E)carbon dioxide
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26
Enzymes that are always present, regardless of the amount of substrate are
A)apoenzymes.
B)axoenzymes.
C)constitutive enzymes.
D)regulated enzymes.
E)endoenzymes.
A)apoenzymes.
B)axoenzymes.
C)constitutive enzymes.
D)regulated enzymes.
E)endoenzymes.
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27
Exergonic reactions
A)include synthesis of large carbohydrates.
B)only occur in heterotrophs.
C)occur during aerobic cellular respiration.
D)do not occur in anaerobic cellular respiration.
E)occur when ADP binds to inorganic phosphate to form ATP.
A)include synthesis of large carbohydrates.
B)only occur in heterotrophs.
C)occur during aerobic cellular respiration.
D)do not occur in anaerobic cellular respiration.
E)occur when ADP binds to inorganic phosphate to form ATP.
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28
Glycolysis
A)uses 2 ATP, produces 2 ATP and requires oxygen.
B)uses 2 ATP, produces 4 ATP and requires oxygen.
C)uses 2 ATP, produces 4 ATP, does not require oxygen.
D)uses 2 ATP, produces 2 ATP, does not require oxygen.
A)uses 2 ATP, produces 2 ATP and requires oxygen.
B)uses 2 ATP, produces 4 ATP and requires oxygen.
C)uses 2 ATP, produces 4 ATP, does not require oxygen.
D)uses 2 ATP, produces 2 ATP, does not require oxygen.
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29
Metabolic pathways that regenerate their starting point are called _____ pathways.
A)linear
B)bidirectional
C)convergent
D)cyclic
E)divergent
A)linear
B)bidirectional
C)convergent
D)cyclic
E)divergent
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30
Which of the following is not involved in the step that occurs between glycolysis and the Krebs cycle?
A)reduction of NAD
B)decarboxylation of pyruvic acid
C)Coenzyme A attaches to an acetyl group
D)dehydrogenation of pyruvic acid
E)pyruvic acid accepts electrons from NADH
A)reduction of NAD
B)decarboxylation of pyruvic acid
C)Coenzyme A attaches to an acetyl group
D)dehydrogenation of pyruvic acid
E)pyruvic acid accepts electrons from NADH
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31
Substrate-level phosphorylation occurs in
A)glycolysis and electron transport chain.
B)photosynthesis and glycolysis.
C)glycolysis and Krebs cycle.
D)Krebs cycle and electron transport chain.
E)All of these choices are correct.
A)glycolysis and electron transport chain.
B)photosynthesis and glycolysis.
C)glycolysis and Krebs cycle.
D)Krebs cycle and electron transport chain.
E)All of these choices are correct.
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32
FAD, NADP, and NAD are all _____ carriers.
A)hydrogen
B)electron
C)ATP
D)both hydrogen and electron
A)hydrogen
B)electron
C)ATP
D)both hydrogen and electron
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33
Each of the following are denaturing agents except
A)high temperature.
B)low temperature.
C)high pH.
D)low pH.
E)All of the choices are correct.
A)high temperature.
B)low temperature.
C)high pH.
D)low pH.
E)All of the choices are correct.
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34
In the cell, energy released by electrons is often used to phosphorylate
A)ATP.
B)ADP.
C)pyruvic acid.
D)oxygen.
E)NAD.
A)ATP.
B)ADP.
C)pyruvic acid.
D)oxygen.
E)NAD.
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35
The step involving ATP, hexokinase and the phosphorylation of glucose to glucose-6-phosphate is
A)the final step of the Krebs cycle.
B)the first redox reaction of the electron transport system.
C)an example of oxidative phosphorylation.
D)an example of substrate-level phosphorylation.
E)an example of photophosphorylation.
A)the final step of the Krebs cycle.
B)the first redox reaction of the electron transport system.
C)an example of oxidative phosphorylation.
D)an example of substrate-level phosphorylation.
E)an example of photophosphorylation.
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36
The formation of citric acid from oxaloacetic acid and an acetyl group begins
A)glycolysis.
B)the electron transport system.
C)the Krebs cycle.
D)fermentation.
E)oxidative phosphorylation.
A)glycolysis.
B)the electron transport system.
C)the Krebs cycle.
D)fermentation.
E)oxidative phosphorylation.
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37
Which of the following is not a process that regenerates ATP?
A)oxidative phosphorylation
B)reductive phosphorylation
C)substrate-level phosphorylation
D)photophosphorylation
A)oxidative phosphorylation
B)reductive phosphorylation
C)substrate-level phosphorylation
D)photophosphorylation
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38
All of the following pertain to glycolysis EXCEPT
A)occurs without oxygen.
B)ends with formation of pyruvic acid.
C)occurs during fermentation.
D)degrades glucose to CO2 and H2O.
E)involves reduction of NAD.
A)occurs without oxygen.
B)ends with formation of pyruvic acid.
C)occurs during fermentation.
D)degrades glucose to CO2 and H2O.
E)involves reduction of NAD.
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39
When the product of reaction A becomes the reactant of reaction B, the metabolic pathway is
A)linear.
B)bidirectional.
C)convergent.
D)cyclic.
E)divergent.
A)linear.
B)bidirectional.
C)convergent.
D)cyclic.
E)divergent.
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40
Enzymes that function inside a cell are
A)apoenzymes.
B)exoenzymes.
C)constitutive enzymes.
D)regulated enzymes.
E)endoenzymes.
A)apoenzymes.
B)exoenzymes.
C)constitutive enzymes.
D)regulated enzymes.
E)endoenzymes.
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41
The reactions of fermentation function to regenerate _____ molecules for use in glycolysis.
A)pyruvic acid
B)ATP
C)NAD
D)NADH
E)glucose
A)pyruvic acid
B)ATP
C)NAD
D)NADH
E)glucose
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42
How many carbon dioxide molecules are produced by the complete aerobic breakdown of one glucose molecule?
A)2
B)3
C)4
D)5
E)6
A)2
B)3
C)4
D)5
E)6
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43
The majority of reduced NAD is produced in
A)glycolysis.
B)Krebs cycle.
C)the electron transport chain.
D)photosynthesis.
A)glycolysis.
B)Krebs cycle.
C)the electron transport chain.
D)photosynthesis.
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44
As the electron transport carriers shuttle electrons, they actively pump _____ into the outer membrane compartment setting up a concentration gradient called the proton motive force.
A)ATP
B)phosphate
C)hydrogen ions
D)oxygen
E)NADH
A)ATP
B)phosphate
C)hydrogen ions
D)oxygen
E)NADH
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45
An oxidase detection test can be used to identify certain bacteria because they lack
A)Cytochrome c oxidase.
B)NAD.
C)mitochondria.
D)ATP synthase.
E)Coenzyme Q.
A)Cytochrome c oxidase.
B)NAD.
C)mitochondria.
D)ATP synthase.
E)Coenzyme Q.
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46
In bacterial cells, the electron transport system is located in the
A)cell membrane.
B)mitochondria.
C)chloroplasts.
D)ribosomes.
E)cytoplasm.
A)cell membrane.
B)mitochondria.
C)chloroplasts.
D)ribosomes.
E)cytoplasm.
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47
Fermentation
A)requires an organic electron acceptor.
B)requires oxygen.
C)only occurs in aerobic organisms.
D)is equivalent to aerobic respiration in ATP production.
A)requires an organic electron acceptor.
B)requires oxygen.
C)only occurs in aerobic organisms.
D)is equivalent to aerobic respiration in ATP production.
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48
Reduction of nitrogen-oxygen ions and compounds by some bacteria is called
A)aerobic respiration.
B)denitrification.
C)nitrification.
D)fermentation.
E)deamination.
A)aerobic respiration.
B)denitrification.
C)nitrification.
D)fermentation.
E)deamination.
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49
Each FADH2 from the Krebs cycle enters the electron transport system and gives rise to _____ ATPs.
A)1
B)2
C)3
D)4
E)5
A)1
B)2
C)3
D)4
E)5
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50
Which of the following are not photosynthetic pigments?
A)carotenoids
B)leukophylls
C)phycobilins
D)chlorophylls
E)All of these choices are correct.
A)carotenoids
B)leukophylls
C)phycobilins
D)chlorophylls
E)All of these choices are correct.
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51
Mixed acid fermentation
A)produces butyric acid.
B)occurs in all bacteria.
C)produces several different acids plus CO2 and H2 gases.
D)is seen in Streptococcus and Lactobacillus.
E)also produces ethanol.
A)produces butyric acid.
B)occurs in all bacteria.
C)produces several different acids plus CO2 and H2 gases.
D)is seen in Streptococcus and Lactobacillus.
E)also produces ethanol.
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52
The redox carriers of the electron transport chain that have a tightly bound metal atom responsible for accepting and donating electrons are
A)NAD molecules.
B)FAD molecules.
C)NADP molecules.
D)the cytochromes.
E)the flavoproteins.
A)NAD molecules.
B)FAD molecules.
C)NADP molecules.
D)the cytochromes.
E)the flavoproteins.
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53
Each NADH that enters the electron transport system gives rise to _____ ATP.
A)2
B)3
C)24
D)36
E)38
A)2
B)3
C)24
D)36
E)38
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54
In bacterial cells, when glucose is completely oxidized by all the pathways of aerobic cellular respiration, what is the maximum number of ATP generated?
A)2 ATP
B)3 ATP
C)24 ATP
D)36 ATP
E)38 ATP
A)2 ATP
B)3 ATP
C)24 ATP
D)36 ATP
E)38 ATP
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55
When glucose is broken down by glycolysis during bacterial fermentation, what is the net production of ATP?
A)2 ATP
B)3 ATP
C)24 ATP
D)36 ATP
E)38 ATP
A)2 ATP
B)3 ATP
C)24 ATP
D)36 ATP
E)38 ATP
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56
In which pathway is the most NADH generated?
A)electron transport system
B)Krebs cycle
C)glycolysis
D)alcoholic fermentation
E)mixed acid fermentation
A)electron transport system
B)Krebs cycle
C)glycolysis
D)alcoholic fermentation
E)mixed acid fermentation
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57
In which stage of aerobic respiration is water produced?
A)glycolysis
B)Krebs cycle
C)electron transport system
A)glycolysis
B)Krebs cycle
C)electron transport system
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58
During which of the phases of cellular respiration is the majority of ATP formed?
A)electron transport
B)Krebs cycle
C)glycolysis
D)processing of pyruvic acid for the Krebs cycle
E)All phases produce the same number of ATP molecules.
A)electron transport
B)Krebs cycle
C)glycolysis
D)processing of pyruvic acid for the Krebs cycle
E)All phases produce the same number of ATP molecules.
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59
What chemical causes death in many eukaryotes because it blocks Cytochrome c oxidase?
A)copper
B)iron
C)cyanide
D)oxygen
E)carbon monoxide
A)copper
B)iron
C)cyanide
D)oxygen
E)carbon monoxide
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60
Which of the following is not true of anaerobic respiration? v: 05_22_2015_QC_CS-15256
A)involves glycolysis
B)generates some ATP
C)utilizes an electron transport system
D)uses the same final electron acceptors as aerobic respiration
A)involves glycolysis
B)generates some ATP
C)utilizes an electron transport system
D)uses the same final electron acceptors as aerobic respiration
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61
ATP synthase is a complex enzyme needed for oxidative phosphorylation.
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62
ATP is composed of deoxyribose, adenine, and 3 phosphate groups.
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63
Building block molecules for biosynthetic pathways come from the cell's catabolic pathways and from the environment.
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64
Amination is the addition of a(n) _____ group to a carbon skeleton.
A)carboxyl
B)amino
C)phosphate
D)hydroxyl
A)carboxyl
B)amino
C)phosphate
D)hydroxyl
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65
Which of the following are characteristics of the Calvin cycle?
A)The Calvin cycle requires light.
B)Nitrogen is fixed into an organic form during the Calvin cycle.
C)The Calvin cycle produces glucose as an end product.
D)The Calvin cycle produces carbon dioxide and water.
E)All of these choices are correct.
A)The Calvin cycle requires light.
B)Nitrogen is fixed into an organic form during the Calvin cycle.
C)The Calvin cycle produces glucose as an end product.
D)The Calvin cycle produces carbon dioxide and water.
E)All of these choices are correct.
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66
The principle sites of amphibolic interaction occur during
A)glycolysis and photosynthesis.
B)Krebs cycle and electron transport system.
C)glycolysis and Krebs cycle.
D)fermentation and Krebs cycle.
E)fermentation and glycolysis.
A)glycolysis and photosynthesis.
B)Krebs cycle and electron transport system.
C)glycolysis and Krebs cycle.
D)fermentation and Krebs cycle.
E)fermentation and glycolysis.
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67
Photosynthesis occurs in chloroplasts of prokaryotes.
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68
All organisms that are photosynthetic are oxygenic.
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69
Only yeast produces alcohol as a fermentation product.
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70
The best way to control a metabolic pathway is to control the fastest enzyme in the series.
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71
Intermediates from the Krebs cycle can be converted to amino acids by the process of
A)amination.
B)deamination.
C)phosphorylation.
D)beta oxidation.
E)gluconeogenesis.
A)amination.
B)deamination.
C)phosphorylation.
D)beta oxidation.
E)gluconeogenesis.
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72
Many exoenzymes from pathogens are virulence factors.
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73
Glyceraldehyde 3-phosphate is an intermediary of glycolysis that can also be utilized in biosynthetic pathways.
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74
Hydrolysis reactions are catabolic reactions that use water to split the reactant into smaller subunits.
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75
All aerobic bacterial species have identical electron acceptors in their electron transport systems.
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76
Which of the following is not true of the light-dependent reactions of photosynthesis?
A)They occur in the thylakoid membranes.
B)They generates NADPH.
C)They generate glucose from CO2 and H2O.
D)Photons cause magnesium to release electrons.
E)ATP is generated by a chemiosmotic mechanism.
A)They occur in the thylakoid membranes.
B)They generates NADPH.
C)They generate glucose from CO2 and H2O.
D)Photons cause magnesium to release electrons.
E)ATP is generated by a chemiosmotic mechanism.
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77
The process in which organisms integrate catabolic and anabolic pathways to improve cell efficiency is called
A)metabolism.
B)amphibolism.
C)anabolism.
D)catabolism.
E)biosynthesis.
A)metabolism.
B)amphibolism.
C)anabolism.
D)catabolism.
E)biosynthesis.
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78
Denaturing an apoenzyme will destroy the three-dimensional shape of the protein, making it nonfunctional.
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79
Microbes can utilize only carbohydrates for energy production.
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80
ATP molecules are catalysts that lower the activation energy needed to initiate a reaction.
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