Deck 15: Proteomics

Question 1

The primary antibody in a Western blot .

A)binds to the nitrocellulose membrane
B)is conjugated to a detection system
C)is used to block non-specific binding of the secondary antibody
D)binds to the specific protein

Answer 1

D

Question 2

When a virus displays multiple peptide sequences,this is termed .

A)phage array
B)yeast two hybrid system
C)phage display
D)protein microarray

Answer 2

C

Question 3

Why might proteins be tagged?

A)purification
B)identification
C)anchoring
D)all of the above

Answer 3

D

Question 4

What is the proteome?

A)The total set of proteins in the cell at any given point.
B)The number of genes that are expressed in a specific growth condition.
C)The total set of proteins encoded by the organism's genome.
D)The number of expressed genes.

Question 5

All of the following are appropriate methods for investigating protein-protein interactions except .

A)mass spectrometry
B)two hybrid analysis
C)electrophoretic mobility shift assays
D)co-immunoprecipitation

Question 6

A method that uses an antibody to detect a specific protein is called .

A)SDS-PAGE
B)Western blot
C)Mass Spectrometry
D)Yeast two hybrid system

Question 7

Which method allows a researcher to find a gene based upon the protein that it encodes?

A)Co-immunoprecipitation
B)Phage display
C)RT-PCR
D)Intein trapping

Question 8

Inteins .

A)are protein self-splicing sequences
B)are portions of mRNA that are spliced out of the primary transcript
C)are never translated
D)are protein sequences that are part of functional proteins

Question 9

Proteins microarrays .

A)are used to investigate protein-protein interactions
B)are used to determine enzymatic activity
C)help determine the binding of DNA or RNA to protein
D)all of the above

Question 10

How are proteins denatured in SDS-PAGE?

A)detergent and high temperature
B)high temperature only
C)acylamide
D)electrical charge