Deck 8: DNA Sequencing

Question 1

Dideoxy nucleotides are missing a 3' hydroxyl.

Answer 1

True

Question 2

Which of the following is a method for detecting the addition of nucleotides in a sequencing reaction?

A)luminescence
B)fluorescence
C)changes in pH
D)all of the above

Answer 2

D

Question 3

Which sequencing method would work best for determining the sequence of a single DNA molecule?

A)pyrosequencing
B)third generation sequencing
C)second generation sequencing
D)chain termination sequencing

Answer 3

B

Question 4

Nanopore technology is speedy and handles long DNA molecules.

Question 5

All of the following are second generation sequencing methods except .

A)454 sequencing
B)Illumina/Solexa sequencing
C)SOLiD/Applied Biosystems method
D)Pyrosequencing

Question 6

When DNA polymerase inserts a dideoxy nucleotide,.

A)chain elongation continues until the end of the template
B)energy is produced during the reaction
C)chain elongation is terminated
D)a primer is formed for the next round of sequencing

Question 7

Nanopore detectors contain pores that only allow to pass through.

A)hydrogen ions
B)single-stranded DNA
C)double-stranded DNA
D)dideoxy nucleotides

Question 8

DNA chip technology relies on the hybridization of .

A)DNA to DNA
B)DNA to RNA
C)RNA to DNA
D)RNA to RNA

Question 9

Which method is most useful for sequencing short DNA regions from multiple individuals?

A)dideoxy sequencing
B)pyrosequencing
C)primer walking
D)SMRT sequencing

Question 10

DNA moves across nanopore detectors because .

A)an electrical charge is applied
B)there is a high concentration of salt on one side of the channel
C)there is a difference in pH across the membrane
D)it is physically pulled across by enzymes