Deck 6: Polymerase Chain Reaction

Question 1

In real time PCR,the presence of double-stranded DNA is detected by .

A)fluorescence of SYBR® Green
B)luminescence of SYBER® Green
C)quenching of SYBR® Green fluorescence
D)rapid decrease in the amount of SYBR® Green fluorescence

Answer 1

A

Question 2

The enzyme that converts RNA back into a DNA copy called complementary DNA cDNA)is called .

A)DNA polymerase
B)RNA polymerase
C)cDNA polymerase
D)Reverse transcriptase

Answer 2

D

Question 3

RNA can be synthesized in PCR if RNA polymerase and RNA nucleotides are used in place of DNA polymerase and DNA nucleotides,respectively.

Answer 3

False

Question 4

What is the function of the RNA primer during DNA synthesis?

A)DNA polymerase requires a 3' hydroxyl to initiate synthesis.
B)The primer helps the replication machinery find the origin of replication.
C)The primer is required for cleavage of the DNA to generate a loose end.
D)The primer signals DNA polymerase to terminate synthesis.

Question 5

Messenger RNA from eukaryotic cells can be specifically amplified using .

A)Standard PCR
B)RT-PCR
C)Differential display PCR
D)RACE

Question 6

During which step in PCR does synthesis occur?

A)Denaturation
B)Annealing
C)Elongation
D)Termination

Question 7

Why must DNA be denatured during PCR?

A)DNA polymerases work best at higher temperatures?
C)The degraded DNA nucleotides are recycled during the synthesis step of PCR.
D)DNA does not need to be denatured for PCR to work.

Question 8

Why is Escherichia coli DNA polymerase not very useful for PCR?

A)E.coli DNA polymerase cannot proofread during replication.
B)E.coli DNA polymerase only amplifies circular DNA.
C)E.coli DNA polymerase is heat labile.
D)E.coli DNA polymerase is too slow.

Question 9

In addition to DNA polymerase,a PCR reaction mixture contains all of the following except .

A)Buffer
B)Primers
C)Deoxynucleotide triphosphates
D)Ligase

Question 10

Which component of the PCR reaction mixture confers specificity in the reaction for the target sequence on the original DNA template?

A)Taq polymerase
B)primers
C)nucleotides
D)template DNA

Question 11

Which of the following uses an RNA template during the technique?

A)TA cloning
B)RAPD
C)Inverse PCR
D)RT-PCR