Deck 8: Precipitation Reactions

Question 1

An example of a qualitative precipitation procedure is:

A) Ouchterlony
B) radial immunodiffusion
C) rapid plasma reagin
D) complement fixation

Answer 1

Ouchterlony

Question 2

Radial immunodiffusion combined with electrophoresis best describes which of the following?

A) immunofixation electrophoresis
B) immunoelectrophoresis
C) Ouchterlony diffusion
D) rocket immunoelectrophoresis

Answer 2

rocket immunoelectrophoresis

Question 3

In the Mancini endpoint method of RID:

A) The diameter is proportional to the log of the concentration of antigen.
B) The squared diameter is proportional to the concentration of antigen.
C) The squared diameter is proportional to the log of the concentration of antigen.
D) The diameter is directly proportional to the concentration.

Answer 3

The squared diameter is proportional to the concentration of antigen.

Question 4

The strength of binding of one Fab on an immunoglobulin to one epitope on an antigen is referred to as:

A) affinity
B) inflammation
C) diapedesis

Question 5

An Ouchterlony immunodiffusion assay is set up and a line of precipitation is seen forming between the antigen and antibody wells.The lines of precipitation formed by two adjacent antigen wells cross each other,forming an X.The reaction described is called:

A) identity
B) non-identity
C) partial identity

Question 6

Which of the following might adversely affect an electrophoresis reaction?

A) decreasing the strength of the current
B) changing the pH of the buffer
C) increasing the strength of the current
D) all of the above
E) all except one of the above

Question 7

In which of the following zones is a false-negative reaction likely?

A) prozone
B) zone of equivalence
C) postzone
D) all of the above

Question 8

Your supervisor wants you to design a quantitative precipitation assay for a new diagnostic test.Which of the following methods would you adopt?

A) Ouchterlony immunodiffusion
B) serum protein electrophoresis
C) radial immunodiffusion
D) enzyme immunoassay

Question 9

Where does lattice formation occur to the greatest extent?

A) prozone
B) postzone
C) zone of equivalence
D) prezone

Question 10

When antibody combines with a particulate antigen,this is classified as which type of reaction?

A) precipitation
B) agglutination
C) flocculation
D) turbidity

Question 11

The zone at which antibody and antigen are at optimal concentrations so that they form a large insoluble lattice together is called the:

A) equivalence zone
B) postzone
C) prozone

Question 12

A situation in which antigen is in excess as compared to antibody is called:

A) prozone
B) postzone
C) equivalence zone

Question 13

A situation in which antibody is in excess as compared to antigen is called:

A) postzone
B) prozone
C) equivalence zone

Question 14

Which technique is best to determine if two fungal antigens are identical?

A) Ouchterlony immunodiffusion
B) serum electrophoresis
C) rocket electrophoresis
D) radial immunodiffusion

Question 15

The initial force of attraction between an antigen and an antibody is called:

A) affinity
B) avidity
C) equivalence
D) Van der Waals force

Question 16

In radial immunodiffusion,the diameter of the precipitin ring correlates with the:

A) concentration of antibody
B) size of antigen
C) length of time the antigen and antibody are bound
D) concentration of antigen

Question 17

Affinity between individual antigen and antibody molecules depends on several types of bonds,such as ionic bonds,hydrophobic bonds,hydrogen bonds,and van der Waals forces.How is the strength of these attractions characterized?

A) specificity
B) avidity
C) reactivity
D) multivalency

Question 18

The radial immunodiffusion test is a method involving:

A) agglutination
B) flocculation
C) precipitation
D) neutralization

Question 19

A serological reaction is set up in which the antigen and antibody are both soluble and form an insoluble complex that is detected macroscopically.What type of assay was described?

A) precipitation
B) agglutination
C) flocculation
D) neutralization

Question 20

In an Ouchterlony immunodiffusion procedure,a continuous arc of precipitate is observed between two adjacent wells.This is a reaction of:

A) identity
B) non-identity
C) partial identity

Question 21

Electrophoresis followed by diffusion of antibody from wells cut in the gel best describes which of the following?

A) immunofixation electrophoresis
B) immunoelectrophoresis
C) Ouchterlony diffusion
D) rocket immunodiffusion

Question 22

Which of the following would be considered a single diffusion reaction?

A) RID
B) immunoelectrophoresis
C) Ouchterlony diffusion
D) immunofixation electrophoresis

Question 23

Measurement of light scattered at an angle used to quantitate antigen-antibody complexes best describes:

A) nephelometry
B) turbidimetry
C) spectrophotometry
D) flow cytometry

Question 24

The Western blot test used to confirm HIV is an example of which of the following?

A) immunofixation
B) immunoelectrophoresis
C) double diffusion
D) passive agglutination

Question 25

Precipitation reactions involve combination of soluble antibody with:

A) soluble antigen
B) cellular antigens
C) red blood cells
D) radioactive antigen

Question 26

The force that keeps antigen and antibody together after the initial attraction and which is responsible for stability of the antigen-antibody complex is:

A) affinity
B) polarity
C) avidity
D) Van der Waals force