Deck 11: How Genes Are Controlled
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Deck 11: How Genes Are Controlled
1
In a prokaryote, a group of genes with related functions, along with their associated control sequences, defines a(n)
A) allele.
B) operon.
C) promoter.
D) repressor.
A) allele.
B) operon.
C) promoter.
D) repressor.
B
2
Both prokaryotic and eukaryotic cells use ________ to turn certain genes on or off.
A) activators
B) enhancers
C) transcription factors
D) RNA polymerase
A) activators
B) enhancers
C) transcription factors
D) RNA polymerase
C
3
The relationship between DNA and histones is most like
A) an egg yolk inside of an egg.
B) a spoon cradling some peas.
C) thread wrapped around a spool.
D) the candy shell surrounding the chocolate in a piece of M&M candy.
A) an egg yolk inside of an egg.
B) a spoon cradling some peas.
C) thread wrapped around a spool.
D) the candy shell surrounding the chocolate in a piece of M&M candy.
C
4
The expression of the tryptophan operon is controlled by a(n)
A) repressor that is active when it is alone.
B) repressor that is inactive when it binds to lactose.
C) repressor that is active when it binds to tryptophan.
D) activator that turns the operon on by binding to DNA.
A) repressor that is active when it is alone.
B) repressor that is inactive when it binds to lactose.
C) repressor that is active when it binds to tryptophan.
D) activator that turns the operon on by binding to DNA.
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5
There is a mutation in the operator of the trp operon in a cell such that the trp repressor is unable to bind to the operator. If tryptophan is added to the cell, what will happen?
A) Tryptophan will bind to the repressor, and trp enzymes will be produced.
B) Tryptophan will bind to the repressor, and trp enzymes will not be produced.
C) Tryptophan will not bind to the repressor, and trp enzymes will be produced.
D) Tryptophan will bind to the operator, and trp enzymes will be produced.
A) Tryptophan will bind to the repressor, and trp enzymes will be produced.
B) Tryptophan will bind to the repressor, and trp enzymes will not be produced.
C) Tryptophan will not bind to the repressor, and trp enzymes will be produced.
D) Tryptophan will bind to the operator, and trp enzymes will be produced.
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6
Enhancers are
A) adjacent to the gene that they regulate.
B) required to turn on gene expression when transcription factors are in short supply.
C) DNA sequences to which activator proteins bind.
D) required to facilitate the binding of DNA polymerases.
A) adjacent to the gene that they regulate.
B) required to turn on gene expression when transcription factors are in short supply.
C) DNA sequences to which activator proteins bind.
D) required to facilitate the binding of DNA polymerases.
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7
The tortoiseshell pattern on a cat
A) usually occurs in males.
B) is the result of a homozygous recessive condition.
C) results from X chromosome inactivation.
D) is a result of alleles on the Y chromosome.
A) usually occurs in males.
B) is the result of a homozygous recessive condition.
C) results from X chromosome inactivation.
D) is a result of alleles on the Y chromosome.
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8
The lac operon of E. coli is ________ when the repressor is bound to ________.
A) active; lactose
B) inactive; lactose
C) active; tryptophan
D) inactive; tryptophan
A) active; lactose
B) inactive; lactose
C) active; tryptophan
D) inactive; tryptophan
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9
Proteins that bind to DNA and turn on operons by making it easier for RNA polymerase to bind to a promoter are called
A) regulators.
B) operators.
C) activators.
D) repressors.
A) regulators.
B) operators.
C) activators.
D) repressors.
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10
The coding regions of a gene are called
A) introns.
B) exons.
C) promoters.
D) enhancers.
A) introns.
B) exons.
C) promoters.
D) enhancers.
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11
The term gene expression refers to the
A) fact that each individual of a species has a unique set of genes.
B) fact that individuals of the same species have different phenotypes.
C) process by which genetic information flows from genes to proteins.
D) flow of information from parent to offspring.
A) fact that each individual of a species has a unique set of genes.
B) fact that individuals of the same species have different phenotypes.
C) process by which genetic information flows from genes to proteins.
D) flow of information from parent to offspring.
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12
In female mammals, the inactive X chromosome in each cell
A) can be activated if mutations occur in the active X chromosome.
B) is broken down, and its nucleotides are degraded and reused.
C) is absorbed and used in energy production.
D) becomes a Barr body.
A) can be activated if mutations occur in the active X chromosome.
B) is broken down, and its nucleotides are degraded and reused.
C) is absorbed and used in energy production.
D) becomes a Barr body.
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13
The genes for the enzymes of glycolysis
A) are present in all metabolizing cells, but the genes for specialized proteins are expressed only in particular cell types.
B) are present in all metabolizing cells, and the genes for specialized proteins are expressed in all cell types.
C) and the genes for all specialized proteins are expressed in all metabolizing cells.
D) and the genes for all specialized proteins are expressed in all embryonic cells.
A) are present in all metabolizing cells, but the genes for specialized proteins are expressed only in particular cell types.
B) are present in all metabolizing cells, and the genes for specialized proteins are expressed in all cell types.
C) and the genes for all specialized proteins are expressed in all metabolizing cells.
D) and the genes for all specialized proteins are expressed in all embryonic cells.
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14
A gene operon consists of
A) a transcribed gene only.
B) a promoter only.
C) a regulatory gene only.
D) transcribed genes, an operator, and a promoter.
A) a transcribed gene only.
B) a promoter only.
C) a regulatory gene only.
D) transcribed genes, an operator, and a promoter.
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15
Which is likely to occur in E. coli cells that are grown in milk?
A) The lac operon is shut off, and the cells will not produce lactose-utilizing enzymes.
B) The trp repressor is activated, and the cells will produce lactose-utilizing enzymes.
C) The trp operon is turned on, but the bacteria will not produce lactose-utilizing enzymes.
D) The trp operon and the lac operon are both switched off.
A) The lac operon is shut off, and the cells will not produce lactose-utilizing enzymes.
B) The trp repressor is activated, and the cells will produce lactose-utilizing enzymes.
C) The trp operon is turned on, but the bacteria will not produce lactose-utilizing enzymes.
D) The trp operon and the lac operon are both switched off.
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16
Which permits a single gene to code for more than one polypeptide?
A) retention of different introns in the final version of the different mRNA strands
B) alternative RNA splicing
C) differentiation
D) addition of different types of caps and tails to the final version of the mRNA strands
A) retention of different introns in the final version of the different mRNA strands
B) alternative RNA splicing
C) differentiation
D) addition of different types of caps and tails to the final version of the mRNA strands
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17
Which statement regarding DNA packing is false?
A) A nucleosome consists of DNA wound around a protein core of eight histone molecules.
B) DNA packing tends to promote gene expression.
C) Highly compacted chromatin is generally not expressed at all.
D) Prokaryotes have proteins analogous to histones.
A) A nucleosome consists of DNA wound around a protein core of eight histone molecules.
B) DNA packing tends to promote gene expression.
C) Highly compacted chromatin is generally not expressed at all.
D) Prokaryotes have proteins analogous to histones.
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18
The lac operon in Escherichia coli
A) prevents lactose-utilizing enzymes from being expressed when lactose is absent from the environment.
B) prevents lactose intolerance.
C) prevents lactose-utilizing enzymes from being expressed when lactose is present in the environment.
D) promotes the expression of lactose-utilizing enzymes when lactose is absent from the environment.
A) prevents lactose-utilizing enzymes from being expressed when lactose is absent from the environment.
B) prevents lactose intolerance.
C) prevents lactose-utilizing enzymes from being expressed when lactose is present in the environment.
D) promotes the expression of lactose-utilizing enzymes when lactose is absent from the environment.
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19
RNA splicing involves the
A) addition of a nucleotide "cap" to the molecule.
B) addition of a nucleotide "tail" to the molecule.
C) removal of introns from the molecule.
D) removal of exons from the molecule.
A) addition of a nucleotide "cap" to the molecule.
B) addition of a nucleotide "tail" to the molecule.
C) removal of introns from the molecule.
D) removal of exons from the molecule.
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20
A single cell, the zygote, can develop into an entirely new organism with many different specialized cells. Which of the following statements about this process is false?
A) Additional genetic information for the formation of specialized cells is passed on to the developing embryo via the mother.
B) The descendant cells specialize by a process known as differentiation.
C) The zygote contains all of the genetic information required for the development of many different cell types.
D) Differentiation of the zygote into a multicellular organism results from selective gene expression.
A) Additional genetic information for the formation of specialized cells is passed on to the developing embryo via the mother.
B) The descendant cells specialize by a process known as differentiation.
C) The zygote contains all of the genetic information required for the development of many different cell types.
D) Differentiation of the zygote into a multicellular organism results from selective gene expression.
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21
Which possible use of reproductive cloning is still considered by most to be an unresolved ethical issue?
A) the production of genetically identical animals for experimentation
B) cloning mammals for the production of potentially valuable drugs
C) the production of organs in cloned pigs for transplant into humans
D) the reproductive cloning of humans
A) the production of genetically identical animals for experimentation
B) cloning mammals for the production of potentially valuable drugs
C) the production of organs in cloned pigs for transplant into humans
D) the reproductive cloning of humans
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22
Which statement about microarrays is false?
A) Microarrays enable scientists to determine the activity of thousands of genes at once.
B) Microarrays use tiny portions of double-stranded RNA fragments from a large number of genes.
C) Microarrays are used to determine which genes are active in different tissues or in tissues of different states of health.
D) Microarrays use fluorescently labeled cDNA molecules to identify particular genes expressed at a particular time.
A) Microarrays enable scientists to determine the activity of thousands of genes at once.
B) Microarrays use tiny portions of double-stranded RNA fragments from a large number of genes.
C) Microarrays are used to determine which genes are active in different tissues or in tissues of different states of health.
D) Microarrays use fluorescently labeled cDNA molecules to identify particular genes expressed at a particular time.
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23
Yeasts are able to communicate with each other
A) by close cell-to-cell contact.
B) through chemical signaling.
C) only if they can touch each other and have merged cell walls.
A) by close cell-to-cell contact.
B) through chemical signaling.
C) only if they can touch each other and have merged cell walls.
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24
A homeotic gene
A) determines which end of the egg will become the head and which end will become the tail.
B) serves as a master control gene that functions during embryonic development by controlling the developmental fate of groups of cells.
C) represses gene transcription and promotes mRNA translation.
D) is found only in adult somatic cells.
A) determines which end of the egg will become the head and which end will become the tail.
B) serves as a master control gene that functions during embryonic development by controlling the developmental fate of groups of cells.
C) represses gene transcription and promotes mRNA translation.
D) is found only in adult somatic cells.
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25
A signal outside a cell triggers changes in the transcription and translation inside the cell through
A) post-translational editing.
B) signal transduction pathways.
C) protein breakdown.
A) post-translational editing.
B) signal transduction pathways.
C) protein breakdown.
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26
Which statement about fruit fly development is false?
A) One of the earliest development events is the determination of the head and tail ends of the egg.
B) The location of the head and tail ends of the egg is primarily determined by the location of sperm entry during fertilization.
C) Homeotic genes regulate batteries of other genes that direct the anatomical identity of body parts.
D) Cascades of gene expression routinely direct fruit fly development.
A) One of the earliest development events is the determination of the head and tail ends of the egg.
B) The location of the head and tail ends of the egg is primarily determined by the location of sperm entry during fertilization.
C) Homeotic genes regulate batteries of other genes that direct the anatomical identity of body parts.
D) Cascades of gene expression routinely direct fruit fly development.
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27
Which mechanism of controlling gene expression occurs outside of the nucleus?
A) adding a cap and tail to RNA
B) DNA packing/unpacking
C) RNA splicing
D) translation
A) adding a cap and tail to RNA
B) DNA packing/unpacking
C) RNA splicing
D) translation
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28
Which process occurs when a salamander regenerates a lost limb?
A) The homeotic genes of the regenerating limb are expressed.
B) Certain cells in the limb dedifferentiate, divide, and then redifferentiate to form a new limb.
C) A new salamander develops from the lost limb.
D) The homeotic genes of the regenerating cells turn off.
A) The homeotic genes of the regenerating limb are expressed.
B) Certain cells in the limb dedifferentiate, divide, and then redifferentiate to form a new limb.
C) A new salamander develops from the lost limb.
D) The homeotic genes of the regenerating cells turn off.
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29
In plants, most differentiated cells retain
A) only a tiny fraction of their original set of genes.
B) a complete set of their genes but lose the ability to express most of those genes.
C) a complete set of their genes and retain the ability to express those genes under certain circumstances.
D) the ability to dedifferentiate but then cannot return to their original differentiated state.
A) only a tiny fraction of their original set of genes.
B) a complete set of their genes but lose the ability to express most of those genes.
C) a complete set of their genes and retain the ability to express those genes under certain circumstances.
D) the ability to dedifferentiate but then cannot return to their original differentiated state.
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30
To initiate a signal transduction pathway, a signal binds to a receptor protein usually located in the
A) nucleus.
B) plasma membrane.
C) ER.
D) cytoplasm.
A) nucleus.
B) plasma membrane.
C) ER.
D) cytoplasm.
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31
Transcription factors attach to
A) DNA.
B) the nuclear envelope.
C) plasma membrane receptors.
D) mRNA.
A) DNA.
B) the nuclear envelope.
C) plasma membrane receptors.
D) mRNA.
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32
Why can some plants be cloned from a single cell?
A) Plant cells do not differentiate even when mature, so any cell can grow into an entire plant.
B) Plant cells can dedifferentiate and give rise to all of the specialized cells required to produce an entire plant.
C) Plant cells can produce genes to replace those lost during development.
D) Plant cells are capable of self-renewal by utilizing cellular components from adjacent cells.
A) Plant cells do not differentiate even when mature, so any cell can grow into an entire plant.
B) Plant cells can dedifferentiate and give rise to all of the specialized cells required to produce an entire plant.
C) Plant cells can produce genes to replace those lost during development.
D) Plant cells are capable of self-renewal by utilizing cellular components from adjacent cells.
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33
Signal transduction pathways
A) are found strictly in multicellular organisms, which require cell-to-cell communication.
B) originally evolved in vertebrates.
C) are mechanisms of communication that probably evolved in ancient prokaryotes.
A) are found strictly in multicellular organisms, which require cell-to-cell communication.
B) originally evolved in vertebrates.
C) are mechanisms of communication that probably evolved in ancient prokaryotes.
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34
The cloning of Dolly the sheep
A) demonstrated that the nuclei from differentiated mammalian cells can retain their full genetic potential.
B) demonstrated that differentiated cells contain only a fraction of their full genetic potential.
C) demonstrated, for the first time, that eggs are haploid and body cells are diploid.
D) revealed that cloned mammals most resemble the egg donor.
A) demonstrated that the nuclei from differentiated mammalian cells can retain their full genetic potential.
B) demonstrated that differentiated cells contain only a fraction of their full genetic potential.
C) demonstrated, for the first time, that eggs are haploid and body cells are diploid.
D) revealed that cloned mammals most resemble the egg donor.
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35
RNA interference (RNAi) can be used by
A) researchers to induce the production of more mRNA.
B) researchers to artificially turn on gene expression.
C) viruses to stop the production of new proteins.
D) cells to prevent infections from double-stranded RNA viruses.
A) researchers to induce the production of more mRNA.
B) researchers to artificially turn on gene expression.
C) viruses to stop the production of new proteins.
D) cells to prevent infections from double-stranded RNA viruses.
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36
All of the following mechanisms are used to regulate protein production except
A) protein activation.
B) protein breakdown.
C) DNA replication.
D) the breakdown of mRNA.
A) protein activation.
B) protein breakdown.
C) DNA replication.
D) the breakdown of mRNA.
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37
The use of cloning to produce special embryonic stem cells is called
A) regeneration.
B) reproductive cloning.
C) therapeutic cloning.
A) regeneration.
B) reproductive cloning.
C) therapeutic cloning.
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38
In multicellular organisms, the coordination of cellular activities relies on
A) cell receptors that detect transcription factors.
B) the availability of certain "key" nutrients as cells divide.
C) operons.
D) cell-to-cell signaling and signal transduction pathways.
A) cell receptors that detect transcription factors.
B) the availability of certain "key" nutrients as cells divide.
C) operons.
D) cell-to-cell signaling and signal transduction pathways.
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39
Small pieces of RNA that can regulate translation of mRNA are called
A) microRNA.
B) ribosomal RNA.
C) transfer RNA.
D) messenger RNA.
A) microRNA.
B) ribosomal RNA.
C) transfer RNA.
D) messenger RNA.
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40
Which mammal has not yet been cloned and brought through the complete gestation cycle?
A) cow
B) human
C) pig
D) cat
A) cow
B) human
C) pig
D) cat
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41
The carcinogen known to cause the most cases and types of cancer is
A) X-rays.
B) ultraviolet light.
C) alcohol.
D) tobacco.
A) X-rays.
B) ultraviolet light.
C) alcohol.
D) tobacco.
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42
Which statement about proto-oncogenes is false?
A) Proto-oncogenes are normal genes with the potential to become oncogenes.
B) Many proto-oncogenes code for growth factors.
C) A mutation must occur in a cell's DNA for a proto-oncogene to become an oncogene.
D) If a proto-oncogene's gene product is produced in smaller quantities, a cell may become cancerous.
A) Proto-oncogenes are normal genes with the potential to become oncogenes.
B) Many proto-oncogenes code for growth factors.
C) A mutation must occur in a cell's DNA for a proto-oncogene to become an oncogene.
D) If a proto-oncogene's gene product is produced in smaller quantities, a cell may become cancerous.
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43
A normal muscle cell produces the myosin protein, which allows muscles to contract. You discover a single muscle cell that has tRNAs that do not bind to amino acids. Will the myosin gene be expressed in this muscle cell? Why or why not?
A) yes, because all cells in the body express the myosin gene all of the time
B) no, because if the tRNAs do not bind amino acids, then translation cannot occur
C) yes, because the myosin gene will be transcribed in the muscle cell nucleus
D) no, because the myosin mRNA will likely get broken down in the cytoplasm
A) yes, because all cells in the body express the myosin gene all of the time
B) no, because if the tRNAs do not bind amino acids, then translation cannot occur
C) yes, because the myosin gene will be transcribed in the muscle cell nucleus
D) no, because the myosin mRNA will likely get broken down in the cytoplasm
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44
The development of colon cancer occurs slowly, and colon cancer is more frequently seen in the elderly than the young. This is most likely because
A) cancer cells don't have mitochondria, so they grow slowly.
B) usually six or more somatic mutations must occur to give rise to the cancer, which takes time.
C) cancer cells have to wait until new blood vessels grow into the area, which takes much time.
D) most cancer mutations interfere with mitosis, so cell division occurs more slowly.
A) cancer cells don't have mitochondria, so they grow slowly.
B) usually six or more somatic mutations must occur to give rise to the cancer, which takes time.
C) cancer cells have to wait until new blood vessels grow into the area, which takes much time.
D) most cancer mutations interfere with mitosis, so cell division occurs more slowly.
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45
Adult stem cells have limited therapeutic potential
A) because they are fully differentiated.
B) because they lack a complete set of genes.
C) due to their excessive numbers in tissues.
D) because their developmental potential is limited to certain tissues.
A) because they are fully differentiated.
B) because they lack a complete set of genes.
C) due to their excessive numbers in tissues.
D) because their developmental potential is limited to certain tissues.
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46
A cell has a mutation in both alleles of its p53 gene that causes the p53 protein to be three times as effective as normal. What do you predict would happen to this cell?
A) The cell would divide three times as fast and would lead to cancer.
B) The cell would divide into six daughter cells instead of two and would lead to cancer.
C) The cell would divide with mutated DNA and would lead to cancer.
D) The cell would divide normally or possibly not at all.
A) The cell would divide three times as fast and would lead to cancer.
B) The cell would divide into six daughter cells instead of two and would lead to cancer.
C) The cell would divide with mutated DNA and would lead to cancer.
D) The cell would divide normally or possibly not at all.
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47
Scientists are attempting to make a chicken look like a dinosaur ("dino-chicken") by using drugs or proteins to turn on certain homeotic genes in somatic cells during the development of a chicken embryo. If the scientists are successful in creating both male and female "dino-chickens," and then the "dino-chickens" mate, what type of offspring would the two "dino-chickens" have?
A) a male or female "dino-chicken"
B) a male or female normal chicken
C) You can't know for sure because of random fertilization.
D) You can't know for sure because it depends on whether the modified homeotic genes are dominant or recessive.
A) a male or female "dino-chicken"
B) a male or female normal chicken
C) You can't know for sure because of random fertilization.
D) You can't know for sure because it depends on whether the modified homeotic genes are dominant or recessive.
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48
Which molecules are not required to express a gene in eukaryotic cells?
A) RNA polymerase
B) DNA-bending protein
C) activator protein
D) repressor protein
A) RNA polymerase
B) DNA-bending protein
C) activator protein
D) repressor protein
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49
E. coli can synthesize the amino acid threonine or absorb it from the environment. In the thr operon, the repressor is active when it is bound to threonine. If an E. coli bacterium is in an environment where threonine is absent, will transcription of threonine synthesis genes take place? Why or why not?
A) yes, because threonine will not be bound to the repressor, so the repressor will be inactive
B) no, because threonine will not be bound to the repressor, so the repressor will be inactive
C) yes, because threonine will not be bound to the repressor, so the repressor will be active
D) no, because threonine will not be bound to the repressor, so the repressor will be active
A) yes, because threonine will not be bound to the repressor, so the repressor will be inactive
B) no, because threonine will not be bound to the repressor, so the repressor will be inactive
C) yes, because threonine will not be bound to the repressor, so the repressor will be active
D) no, because threonine will not be bound to the repressor, so the repressor will be active
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50
There is a mutation in a promoter next to a gene such that RNA polymerase can never bind. What steps must occur for the gene to be transcribed?
A) An activator protein must bind an enhancer.
B) The DNA must physically bend so that the activator is close to the promoter.
C) Transcription factors must bind to the activator and promoter.
D) Transcription will never take place in this specific example.
A) An activator protein must bind an enhancer.
B) The DNA must physically bend so that the activator is close to the promoter.
C) Transcription factors must bind to the activator and promoter.
D) Transcription will never take place in this specific example.
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51
Which of the following statements regarding cancer risk factors is false?
A) Factors that alter DNA and make cells cancerous are called carcinogens.
B) Mutagens are usually not carcinogens.
C) X-rays and ultraviolet radiation are two of the most potent carcinogens.
D) Eating 20-30 grams of plant fiber daily and reducing the intake of animal fat can reduce your risk of developing colon cancer.
A) Factors that alter DNA and make cells cancerous are called carcinogens.
B) Mutagens are usually not carcinogens.
C) X-rays and ultraviolet radiation are two of the most potent carcinogens.
D) Eating 20-30 grams of plant fiber daily and reducing the intake of animal fat can reduce your risk of developing colon cancer.
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52
Mutations in the p53 gene can lead to cancer by
A) causing the production of excessive amounts of relay proteins.
B) causing the production of a faulty protein that is no longer able to inhibit cell division.
C) promoting the expression of mRNA that can interact with DNA, resulting in new mutations.
D) increasing the production of growth hormones, which trigger faster cell cycles.
A) causing the production of excessive amounts of relay proteins.
B) causing the production of a faulty protein that is no longer able to inhibit cell division.
C) promoting the expression of mRNA that can interact with DNA, resulting in new mutations.
D) increasing the production of growth hormones, which trigger faster cell cycles.
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53
Mutations in the proto-oncogene ras and the tumor-suppressor gene p53
A) increase protein synthesis by the cell.
B) can improve the chance of avoiding cancer as one ages.
C) can enhance further mutations, which can develop into cancer.
D) disrupt normal regulation of the cell cycle.
A) increase protein synthesis by the cell.
B) can improve the chance of avoiding cancer as one ages.
C) can enhance further mutations, which can develop into cancer.
D) disrupt normal regulation of the cell cycle.
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54
Which is not a factor that contributes to normal cells becoming cancerous?
A) the conversion of a proto-oncogene to an oncogene
B) mutations in a tumor-suppressor gene
C) the production of more effective tumor-suppressor gene products
D) excessive replication of proto-oncogenes
A) the conversion of a proto-oncogene to an oncogene
B) mutations in a tumor-suppressor gene
C) the production of more effective tumor-suppressor gene products
D) excessive replication of proto-oncogenes
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55
A gene that can cause cancer when present in a single copy in a cell is called a(n)
A) oncogene.
B) tumor-suppressor gene.
C) proto-oncogene.
A) oncogene.
B) tumor-suppressor gene.
C) proto-oncogene.
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56
Two yeast cells, one of type a and the other of type α, are in proximity to each other. The a yeast cell has a mutation in the gene for the α factor receptor protein such that the receptor does not translocate to the plasma membrane of the a cell. If both cells secrete their respective a and α factors, which of the following outcomes is most likely to occur?
A) The a and α cells will grow toward each other.
B) The a cell will grow towards the α cell, but the α cell will not grow toward the a cell.
C) The α cell will grow toward the a cell, but the a cell will not grow towards the α cell.
D) The a and α cells will not grow toward each other at all.
A) The a and α cells will grow toward each other.
B) The a cell will grow towards the α cell, but the α cell will not grow toward the a cell.
C) The α cell will grow toward the a cell, but the a cell will not grow towards the α cell.
D) The a and α cells will not grow toward each other at all.
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57
A stomach cell is producing pepsin, an enzyme that hydrolyzes proteins. Which of the following events suggests that gene expression of pepsin has been turned off in the cell?
A) The pepsin protein is folded properly in the cytoplasm.
B) The chromosome with the pepsin gene is tightly coiled.
C) The pepsin mRNA leaves the nucleus and enters the cytoplasm.
D) Activators and RNA polymerases are abundant in the nucleus.
A) The pepsin protein is folded properly in the cytoplasm.
B) The chromosome with the pepsin gene is tightly coiled.
C) The pepsin mRNA leaves the nucleus and enters the cytoplasm.
D) Activators and RNA polymerases are abundant in the nucleus.
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58
There is a mutation in the operator of the lac operon in a cell such that the lac repressor always stays bound to the operator. If lactose is added to the cell, what will happen?
A) Lactose will bind to the repressor, and lac enzymes will be produced.
B) Lactose will bind to the repressor, and lac enzymes will not be produced.
C) Lactose will not bind to the repressor, and lac enzymes will be produced.
D) Lactose will bind to the operator, and lac enzymes will be produced.
A) Lactose will bind to the repressor, and lac enzymes will be produced.
B) Lactose will bind to the repressor, and lac enzymes will not be produced.
C) Lactose will not bind to the repressor, and lac enzymes will be produced.
D) Lactose will bind to the operator, and lac enzymes will be produced.
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59
Cancer of the colon is caused by
A) a single somatic cell gene mutation.
B) several somatic cell gene mutations.
C) a physical rupture of the colon.
D) a diet high in fiber and low in fat.
A) a single somatic cell gene mutation.
B) several somatic cell gene mutations.
C) a physical rupture of the colon.
D) a diet high in fiber and low in fat.
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60
Which statement regarding stem cells is false?
A) Embryonic stem cells can give rise to all the different specialized cells in the body.
B) Adult, but not embryonic, stem cells can be grown in laboratory culture.
C) Adult stem cells are present in adult tissues.
D) Adult stem cells are partway along the road to differentiation.
A) Embryonic stem cells can give rise to all the different specialized cells in the body.
B) Adult, but not embryonic, stem cells can be grown in laboratory culture.
C) Adult stem cells are present in adult tissues.
D) Adult stem cells are partway along the road to differentiation.
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61
After reading the paragraph below, answer the questions that follow.
While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
A pancreatic cell normally produces low levels of the protein insulin, but after a meal it produces large levels of insulin before returning to its initial state. Which of the following figures would represent qPCR data obtained from a pancreatic cell that went through this cycle?
A)

B)

C)

D)

While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
A pancreatic cell normally produces low levels of the protein insulin, but after a meal it produces large levels of insulin before returning to its initial state. Which of the following figures would represent qPCR data obtained from a pancreatic cell that went through this cycle?
A)

B)

C)

D)

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62
Half the trees in an orchard were derived from rootstock "A" and half from rootstock "B," but all the trees had the same scion. If the trees grafted onto rootstock "A" were infected by a parasite that causes blossom rot, the trees grafted onto rootstock "B"
A) would be less likely to become infected because they're grafted onto different rootstocks.
B) would be more likely to become infected, since the pathogen would spread through the soil to the roots of other trees.
C) would be very likely to become infected, because the remaining scions are genetically identical to those that are already infected.
D) There's no way to determine the likelihood of infection, since genetic variability gives all the trees different characteristics.
A) would be less likely to become infected because they're grafted onto different rootstocks.
B) would be more likely to become infected, since the pathogen would spread through the soil to the roots of other trees.
C) would be very likely to become infected, because the remaining scions are genetically identical to those that are already infected.
D) There's no way to determine the likelihood of infection, since genetic variability gives all the trees different characteristics.
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63
There is a mutation in the operator of the trp operon in a cell such that the trp repressor is unable to bind to the operator. If tryptophan is added to the cell, what will happen?
A) Tryptophan will bind to the repressor, and trp enzymes will be produced.
B) Tryptophan will bind to the repressor, and trp enzymes will not be produced.
C) Tryptophan will not bind to the repressor, and trp enzymes will be produced.
D) Tryptophan will bind to the operator, and trp enzymes will be produced.
A) Tryptophan will bind to the repressor, and trp enzymes will be produced.
B) Tryptophan will bind to the repressor, and trp enzymes will not be produced.
C) Tryptophan will not bind to the repressor, and trp enzymes will be produced.
D) Tryptophan will bind to the operator, and trp enzymes will be produced.
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64
The Pax6 gene codes for a transcription factor that initiates differentiation of cells that ultimately form the eye. Suppose that a researcher added an activator for the Pax6 gene to embryonic cells that are in the process of forming legs in Drosophila. What do you think the researcher will observe?
A) Legs and eyes will develop normally.
B) Legs will develop where the eyes normally develop.
C) Eyes will develop where the legs normally develop.
A) Legs and eyes will develop normally.
B) Legs will develop where the eyes normally develop.
C) Eyes will develop where the legs normally develop.
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65
After reading the paragraph below, answer the questions that follow.
While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
A scientist hypothesizes that a new drug turns on a certain gene in a skin cell. Which of the following experiments would be the best to test her hypothesis?
A) Use qPCR to measure the initial levels of gene expression in skin cells, treat skin cells with the drug and measure the levels of gene expression, and then compare the two measurements.
B) Use qPCR to measure the levels of gene expression in skin cells after exposure to the drug.
C) Use qPCR to measure the initial levels of gene expression in muscle cells, treat skin cells with the drug and measure the levels of gene expression, and then compare the two measurements.
D) Use qPCR to measure the levels of gene expression in skin cells after exposure to the drug and then add another drug to turn off gene expression and use qPCR to measure the levels of expression.
While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
A scientist hypothesizes that a new drug turns on a certain gene in a skin cell. Which of the following experiments would be the best to test her hypothesis?
A) Use qPCR to measure the initial levels of gene expression in skin cells, treat skin cells with the drug and measure the levels of gene expression, and then compare the two measurements.
B) Use qPCR to measure the levels of gene expression in skin cells after exposure to the drug.
C) Use qPCR to measure the initial levels of gene expression in muscle cells, treat skin cells with the drug and measure the levels of gene expression, and then compare the two measurements.
D) Use qPCR to measure the levels of gene expression in skin cells after exposure to the drug and then add another drug to turn off gene expression and use qPCR to measure the levels of expression.
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66
What is the correct order of structures in a chromosome from smallest to largest?
A) nucleotide, nucleosome, histone, tight helical fiber, chromosome
B) nucleosome, nucleotide, histone, tight helical fiber, chromosome
C) nucleotide, histone, tight helical fiber, nucleosome, chromosome
D) nucleotide, histone, nucleosome, tight helical fiber, chromosome
A) nucleotide, nucleosome, histone, tight helical fiber, chromosome
B) nucleosome, nucleotide, histone, tight helical fiber, chromosome
C) nucleotide, histone, tight helical fiber, nucleosome, chromosome
D) nucleotide, histone, nucleosome, tight helical fiber, chromosome
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67
In this drawing of the lac operon, which molecule is an inactive repressor? 
A) molecule A
B) molecule B
C) molecule C
D) molecule D

A) molecule A
B) molecule B
C) molecule C
D) molecule D
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68
During an experiment assessing the development of a fly embryo, a scientist artificially turns on a homeotic gene that controls eye development in somatic cells that normally develop into the wings. Which of the following is the most likely outcome of this experiment?
A) A normal adult fly will develop.
B) A mutant adult fly will develop that has eyes on its wings.
C) A mutant adult fly will develop that has legs on its head.
D) A mutant adult fly will develop that has no eyes on its head but has eyes on its wings.
A) A normal adult fly will develop.
B) A mutant adult fly will develop that has eyes on its wings.
C) A mutant adult fly will develop that has legs on its head.
D) A mutant adult fly will develop that has no eyes on its head but has eyes on its wings.
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69
Melanoma (skin cancer) can develop after several mutations occur in skin cells. If a woman is diagnosed with melanoma and becomes pregnant, what are the chances that her offspring will inherit melanoma?
A) The offspring will certainly be born with melanoma.
B) There is a chance, but it depends on how serious the mother's melanoma is.
C) There is a chance, but it depends on whether or not the father has melanoma.
D) It is unlikely that the offspring will be born with melanoma.
A) The offspring will certainly be born with melanoma.
B) There is a chance, but it depends on how serious the mother's melanoma is.
C) There is a chance, but it depends on whether or not the father has melanoma.
D) It is unlikely that the offspring will be born with melanoma.
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70
After reading the paragraph below, answer the questions that follow.
All apples in the United States, regardless of variety or where they're purchased, are produced by cloning. For more than 2,000 years, apple growers around the world have used a type of cloning called grafting to produce larger, better-tasting apples. Why has cloning become the primary method of apple growing? Apples grown from seeds usually don't produce apples with the same taste and appearance as that of the parent tree because there's a high degree of genetic variability among the seeds. Making identical genetic copies of the preferred fruit is the only way to get reliable apple quality.
In grafting, the shoots and branches of the desired fruit, called a scion, are attached onto the trunk and root system of a previously existing tree, called the rootstock. Both components of the graft are needed because the rootstock controls gene expression in the scion, triggering production of apples that match the cloned scion.
Grafting research can be used to produce some interesting tree combinations that are beneficial for intensive agriculture. For example, if you graft the root of a small tree variety, such as the crab apple, onto the shoot from a larger apple tree, such as the Gala, you can produce Gala apples on a much smaller tree. Other scientists are trying to create disease-resistant varieties that would need fewer pesticides. This is beneficial for the environment and also lowers the price of apples in the grocery store.
Why don't the grafted hybrids produce apples with a blend of traits from the scion and the rootstock?
A) The rootstock suppresses activation of the scion genes, which alters fruit production.
B) The rootstock is unable to perform photosynthesis and so can't produce fruit.
C) Transplanted nuclei from scion cells regulate gene expression in the rootstock.
D) The rootstock regulates gene expression in the scion but contributes no genetic information for fruit production.
All apples in the United States, regardless of variety or where they're purchased, are produced by cloning. For more than 2,000 years, apple growers around the world have used a type of cloning called grafting to produce larger, better-tasting apples. Why has cloning become the primary method of apple growing? Apples grown from seeds usually don't produce apples with the same taste and appearance as that of the parent tree because there's a high degree of genetic variability among the seeds. Making identical genetic copies of the preferred fruit is the only way to get reliable apple quality.
In grafting, the shoots and branches of the desired fruit, called a scion, are attached onto the trunk and root system of a previously existing tree, called the rootstock. Both components of the graft are needed because the rootstock controls gene expression in the scion, triggering production of apples that match the cloned scion.
Grafting research can be used to produce some interesting tree combinations that are beneficial for intensive agriculture. For example, if you graft the root of a small tree variety, such as the crab apple, onto the shoot from a larger apple tree, such as the Gala, you can produce Gala apples on a much smaller tree. Other scientists are trying to create disease-resistant varieties that would need fewer pesticides. This is beneficial for the environment and also lowers the price of apples in the grocery store.
Why don't the grafted hybrids produce apples with a blend of traits from the scion and the rootstock?
A) The rootstock suppresses activation of the scion genes, which alters fruit production.
B) The rootstock is unable to perform photosynthesis and so can't produce fruit.
C) Transplanted nuclei from scion cells regulate gene expression in the rootstock.
D) The rootstock regulates gene expression in the scion but contributes no genetic information for fruit production.
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71
Researchers wish to use RNAi to prevent the expression of the enzyme hexokinase in order to study its impact on glycolysis. Which of the following processes would still occur if the researchers used RNAi effectively to silence hexokinase expression?
A) folding of the hexokinase protein
B) translation of the hexokinase protein
C) transcription of the hexokinase gene
A) folding of the hexokinase protein
B) translation of the hexokinase protein
C) transcription of the hexokinase gene
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72
After reading the paragraph below, answer the questions that follow.
While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
In a muscle cell, the contractile protein myosin is present in larger quantities than the structural protein actin. Which of the following qPCR results would support this observation?
A) The fluorescent signal for myosin is higher than that of actin.
B) The fluorescent signal for actin is higher than that of myosin.
C) The fluorescent signals for myosin and actin are equal.
D) The fluorescent signal for myosin is large and is zero for actin.
While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
In a muscle cell, the contractile protein myosin is present in larger quantities than the structural protein actin. Which of the following qPCR results would support this observation?
A) The fluorescent signal for myosin is higher than that of actin.
B) The fluorescent signal for actin is higher than that of myosin.
C) The fluorescent signals for myosin and actin are equal.
D) The fluorescent signal for myosin is large and is zero for actin.
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73
A cell is expressing the mutant form of ras, but one of the downstream relay proteins is not present because of another mutation. How will cell division be affected in this scenario?
A) Cell division will decrease because the mutant form of ras decreases cell division.
B) Cell division will increase because the mutant form of ras increases cell division.
C) The cell will divide normally.
D) The cell will not divide at all unless other proteins that stimulate cell division are present.
A) Cell division will decrease because the mutant form of ras decreases cell division.
B) Cell division will increase because the mutant form of ras increases cell division.
C) The cell will divide normally.
D) The cell will not divide at all unless other proteins that stimulate cell division are present.
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74
In thinking about their role in cell division, oncogenes are like the ________ of a car, while tumor-suppressor genes are like the ________ of a car.
A) gas pedal; brakes
B) steering wheel; brakes
C) gas pedal; headlights
D) brakes; gas pedal
A) gas pedal; brakes
B) steering wheel; brakes
C) gas pedal; headlights
D) brakes; gas pedal
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75
The major function of siRNAs is to
A) increase the rate of protein translation.
B) turn off gene expression.
C) prevent chromatin condensation.
D) support DNA replication.
A) increase the rate of protein translation.
B) turn off gene expression.
C) prevent chromatin condensation.
D) support DNA replication.
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76
What is the proper order of events in the expression of a eukaryotic protein?
A) DNA unpacking, mRNA transport through nucleus, mRNA splicing, protein modification
B) DNA unpacking, mRNA splicing, translation, protein folding
C) transcription, translation, addition of cap and tail to mRNA, DNA unpacking
D) transcription, mRNA splicing, protein modification, translation
A) DNA unpacking, mRNA transport through nucleus, mRNA splicing, protein modification
B) DNA unpacking, mRNA splicing, translation, protein folding
C) transcription, translation, addition of cap and tail to mRNA, DNA unpacking
D) transcription, mRNA splicing, protein modification, translation
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77
All of the trees in an orchard were derived from the same rootstock, but half had scion "A" grafted and the other half had a different scion, scion "B," grafted. The trees that were grafted with scion "A" developed large fruits. What characteristics do you think the fruit from the trees that were grafted with scion "B" will have and why?
A) The fruits will be large because the trees share the same rootstock.
B) The fruits will be large because all scions produce the same kind of fruits.
C) The fruits will be small because the trees share the same rootstock.
D) There is not enough information to know what the fruit will look like.
A) The fruits will be large because the trees share the same rootstock.
B) The fruits will be large because all scions produce the same kind of fruits.
C) The fruits will be small because the trees share the same rootstock.
D) There is not enough information to know what the fruit will look like.
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78
Which structure in this figure shows one complete nucleosome? 
A) structure A
B) structure B
C) structure C
D) structure D

A) structure A
B) structure B
C) structure C
D) structure D
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79
Which statement regarding skin cells and muscle cells in your body is true?
A) Muscle cells contain information about muscle proteins but not about skin color.
B) Skin cells contain information about skin color but not about muscle proteins.
C) Muscle cells contain information about skin color but not about muscle proteins.
D) Skin cells and muscle cells each contain information about both muscle proteins and skin color.
A) Muscle cells contain information about muscle proteins but not about skin color.
B) Skin cells contain information about skin color but not about muscle proteins.
C) Muscle cells contain information about skin color but not about muscle proteins.
D) Skin cells and muscle cells each contain information about both muscle proteins and skin color.
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80
The figure below shows a normal cell transduction pathway. If the receptor weren't there, which of the following is the most likely outcome? 
A) Cell division will occur normally.
B) Cell division will not occur at all.
C) Cell division will be increased.
D) Cell division will be decreased.

A) Cell division will occur normally.
B) Cell division will not occur at all.
C) Cell division will be increased.
D) Cell division will be decreased.
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