Deck 33: Flow Cytometric Analysis in Hematologic Disorders

Question 1

Hydrodynamic focusing in flow cytometry is important because it:

A) Directs the laser light
B) Intensifies the laser light with mirrors
C) Collects the scattered light
D) Creates a core of individually aligned cells

Answer 1

Creates a core of individually aligned cells

Question 2

Forward scatter is related to what cellular feature?

A) Density
B) Size
C) Granularity
D) Complexity

Answer 2

Size

Question 3

A patient has a white cell count of 12.6 *10⁹/L, hematocrit of 28%, and platelet count of 27*10⁹/L. Examination of a Wright-stained blood film reveals that almost all of the white cells in peripheral blood are blasts; the bone marrow is hypercellular with very little fat and is packed with blasts. Flow cytometry is performed on the peripheral blood cells, and a large cluster of cells is present that mark with CD20 and have monoclonal $\kappa$ light chains present on their surface. This patient has a:

A) Myeloid leukemia
B) Mature B-cell malignancy
C) Reactive B-cell hyperplasia
D) Mature T-cell malignancy

Answer 3

Mature B-cell malignancy

Question 4

All of the following are true concerning hematopoietic stem cells except:

A) They express both CD34 and CD117 (c-kit).
B) They lose some antigens as they mature.
C) They express both CD4 and CD34.
D) They gain lineage-specific antigens as they mature.

Question 5

Staining a sample with deoxyribonucleic acid (DNA) dyes such as propidium iodide is done to determine cell:

A) Viability
B) Ploidy
C) Lineage
D) Chromosome number

Question 6

Myeloid stem cells and blasts express which of the following on their surface?

A) CD4
B) CD8
C) CD34
D) Terminal deoxynucleotidyl transferase (TdT)

Question 7

Which of the following is evaluated using fluorochrome dyes that are bound to monoclonal antibodies?

A) Membrane antigens
B) Nuclear complexity
C) Number of lymphocytes
D) Cell size

Question 8

What marker is most closely related to disease progression in acquired immune deficiency syndrome (AIDS) patients?

A) CD3
B) CD4
C) CD8
D) CD10

Question 9

What cell type expresses CD20 and contains immunoglobulin light chains on the surface but not in the cytoplasm?

A) Helper T cells (T_H)
B) T suppressor cells
C) Immature B cells
D) Mature B cells

Question 10

The presence of TdT is characteristic of progenitors of which lineage?

A) Megakaryocytes and platelets
B) Monocytes
C) Erythrocytes
D) Lymphocytes

Question 11

Which of the following is true regarding the relationship between flow cytometry and the microscopic evaluation of leukemic cells?

A) Flow cytometry has made microscopic analysis outmoded.
B) Methods are best used together to establish a diagnosis.
C) More cells can be analyzed by looking under the microscope.
D) The human eye is more sensitive than flow cytometry and better at finding weak antigenic expression.

Question 12

What is an early marker of erythroid precursors?

A) CD45
B) CD71
C) CD34
D) CD8