Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 10

Tumor-suppressor genes are normal human genes that prevent uncontrollable cell growth. Starting with a normal laboratory human cell line, describe how you could use transposon tagging to identify tumor-suppressor genes. Note: When a TE hops into a tumor-suppressor gene, it may cause uncontrolled cell growth. This is detected as a large clump of cells among a normal monolayer of cells.