Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 18

A commercially available strain of P. syringae marketed as Frostban B is used to combat frost damage. This is a naturally occurring strain that carries a loss-of-function mutation in a gene that encodes a protein that promotes frost formation. In addition, researchers have used recombinant DNA techniques to eliminate the function of this gene. Discuss the advantages and disadvantages of using the nonrecombinant strain compared with a recombinant version.