Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 1

Calculating K eq and G from concentrations
An enzymatic hydrolysis of fructose-1-P
Fructose-1-P + H₂ O fructose + Pi
was allowed to proceed to equilibrium at 25°C. The original concentration of fructose-1-P was 0.2 M, but when the system had reached equilibrium the concentration of fructose 1-P was only 6.52 x 10-5 M. Calculate the equilibrium constant for this reaction and the standard free energy of hydrolysis of fructose 1-P.