Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 3

Separating glycated Hb from normal Hb
(Integrates with Chapters 5 and 6.) Human hemoglobin can react with sugars in the blood (usually glucose) to form covalent adducts. The -amino groups of N-terminal valine in the Hb -subunits react with the C-1 (aldehyde) carbons of monosaccharides to form aldimine adducts, which rearrange to form very stable ketoamine products. Quantitation of this "glycated hemoglobin" is important clinically, especially for diabetic individuals. Suggest at least three methods by which glycated Hb could be separated from normal Hb and quantitated.