Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 8

Restriction endonuclease mapping of a DNA fragment
A 10-kb DNA fragment digested with restriction endonuclease Eco RI yielded fragments 4 kb and 6 kb in size. When digested with Bam HI, fragments 1, 3.5, and 5.5 kb were generated. Concomitant digestion with both Eco RI and Bam HI yielded fragments 0.5, 1, 3, and 5.5 kb in size. Give a possible restriction map for the original fragment.