Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 17

Design a sequencing strategy for nucleic acids
Snake venom phosphodiesterase is an a-specific exonuclease (Figure 10.28) that acts equally well on single-stranded RNA or DNA. Design a protocol based on snake venom phosphodiesterase that would allow you to determine the base sequence of an oligonucleotide. Hint: Adapt the strategy for protein sequencing by Edman degradation, as described on pages 10-11 and 114-115.