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Biochemistry 4th Edition by Reginald Garrett, Charles Grisham
Edition 4ISBN: 9780495109358Biochemistry 4th Edition by Reginald Garrett, Charles Grisham
Edition 4ISBN: 9780495109358 Exercise 1
Construction of a recombinant plasmid
A DNA fragment isolated from an Eco RI digest of genomic DNA was combined with a plasmid vector linearized by Eco RI digestion so sticky ends could anneal. Phage T4 DNA ligase was then added to the mixture. List all possible products of the ligation reaction.
A DNA fragment isolated from an Eco RI digest of genomic DNA was combined with a plasmid vector linearized by Eco RI digestion so sticky ends could anneal. Phage T4 DNA ligase was then added to the mixture. List all possible products of the ligation reaction.
Explanation
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Both the DNA fragment and the plasmid we...
Biochemistry 4th Edition by Reginald Garrett, Charles Grisham
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