Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 2

Overlapping restriction sites in a plasmid vector polylinker
The nucleotide sequence of a polylinker in a particular plasmid vector is
-GAATTCCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCThis
polylinker contains restriction sites for Bam HI, Eco RI, Pst I, Sal I, Sma I, Sph I, and Xba I. Indicate the location of each restriction site in this sequence. (See Table 10.2 of restriction enzymes for their cleavage sites.)