Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 7

A polylinker for expression of a -galactosidase fusion protein
Figure shows a polylinker that falls within the -galactosidase coding region of the lacZ gene. This polylinker serves as a cloning site in a fusion protein expression vector where the cloned insert is expressed as a -galactosidase fusion protein. Assume the vector polylinker was cleaved with Bam HI and then ligated with an insert whose sequence reads
GATCCATTTATCCACCGGAGAGCTGGTATCCCCAAAAGACGGCC...
What is the amino acid sequence of the fusion protein Where is the junction between - galactosidase and the sequence encoded by the insert (Consult the genetic code table on the inside front cover to decipher the amino acid sequence.)