Biochemistry 4th Edition by Reginald Garrett, Charles Grisham

Exercise 13

Using antibodies to screen cDNA libraries
You have an antibody against yeast hexokinase A (hexokinase is the first enzyme in the glycolytic pathway). Describe an experimental protocol using the cDNA libraries prepared in problem 11 that would allow you to identify and isolate cDNA for hexokinase. Consulting Chapter 5 for protein analysis protocols, describe an experimental protocol to verify that the protein you have identified is hexokinase A.