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Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill
Edition 4ISBN: 978-0138004644Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill
Edition 4ISBN: 978-0138004644 Exercise 14
The dye ethidium is a planar molecule that can intercalate into double-stranded DNA. This means that it slips between adjacent stacked base pairs. In doing so, it unwinds the DNA helix by about 26° for every ethidium bound.
(a) If ethidium were added to relaxed, closed circular DNA, would negative or positive writhing be expected Explain.
(b) What would be the effect on writhing if the DNA were nicked in one strand
(c) It is observed that progressive addition of ethidium to negatively supercoiled DNA has a peculiar effect: First the eleclrophoretic mobility decreases, but with further addition of ethidium it again increases. Explain.
(a) If ethidium were added to relaxed, closed circular DNA, would negative or positive writhing be expected Explain.
(b) What would be the effect on writhing if the DNA were nicked in one strand
(c) It is observed that progressive addition of ethidium to negatively supercoiled DNA has a peculiar effect: First the eleclrophoretic mobility decreases, but with further addition of ethidium it again increases. Explain.
Explanation
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Verified
(a).
If ethidium were added to relaxed,...
Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill
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