Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill

Exercise 6

Note that some of these problems refer to information presented in the Tools of Biochemistry 5A-D.
Given the following peptide
(a) Estimate the net charge at pH 7 and at pH 12. Assume the p K a values given in Table 5.1.
(b) How many peptides would result if this peptide were treated with (1) cyanogen bromide, (2) trypsin, and (3) chymotrypsin
(c) Suggest a method for separating the peptides produced by chymotrypsin treatment.