Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill

Exercise 1

Design a radiotracer experiment that would allow you to determine which proportion of glucose catabolism in a given tissue preparation occurs through the pentose phosphate pathway and which proportion through glycolysis and the citric acid cycle. Assume that you can synthesize glucose labeled with l4 C in any desired position or combination of positions. Assume also that you can trap CO₂ after administration of labeled glucose and determine its radioactivity.