Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill

Exercise 5

Of the various oxidation reactions in glycolysis and the citric acid cycle, the only one that does not involve NAD + is the succinate dehydrogenase reaction. What would G ° be for an enzyme that oxidizes succinate with NAD + instead of FAD If the intramitochondrial concentration of succinate was 10-fold higher than that of fumarate, what minimum [NAD + ]/[NADH] ratio in mitochondria would be needed to make this reaction exergonic at 37 °C