Biochemistry 4th Edition by Christopher Mathews,Kensal van Holde, Dean Appling, Spencer Anthony Cahill

Exercise 13

To carefully prepared mitochondria were added succinate, oxidized cytochrome c, ADP, orthophosphate, and sodium cyanide. Referring to Figures 15.9 and 15.18, answer the following.
(a) List the sequence of electron carriers in this system.
(b) Write a balanced equation for the overall reaction occurring in this system, showing oxidation of the initial electron donor, reduction of the final acceptor, and synthesis of ATP.
(c) Calculate G ° for the overall reaction. G ° for ATP hydrolysis is 30.5kJ/mol.
(d) Why was cyanide added in this experiment
(c) What would the P/O ratio be if the same experiment were run with addition to the mitochondria of 2,4-dinitrophenol