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Nester's Microbiology 8th Edition by Denise Anderson,Sarah Salm,Deborah Allen
Edition 8ISBN: 978-0073522593Nester's Microbiology 8th Edition by Denise Anderson,Sarah Salm,Deborah Allen
Edition 8ISBN: 978-0073522593 Exercise 1
How did Louis Pasteur help disprove spontaneous generation?
Explanation
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Louis Pasteur was a French chemist during the 19 th century. He disproved the theory of spontaneous generation and won the debate on this topic and Academy's prize. He disproved the notion that a "vital force" originating in the air is responsible for spontaneous generation. Pasteur filtered the air through a gun-cotton filter and observed the presence of microbes on the filter under the microscope.
Pasteur performed an experiment using the swan-neck flasks (long and twisted necks). These flasks can exchange the air inside with the external air, but the microbes from the external air cannot enter into the flask, instead they trapped in the flask of the neck. He sterilized the broth in one of the flasks. With this experiment, he observed that the growth did not occur in the sterile broth.
After that he broke the necks of the flasks and allowed contamination of the broth, microbial growth was observed. Regarding this invention, Pasteur ascertained that "Omne vivum ex vivo" (Life only comes from life).
Pasteur performed an experiment using the swan-neck flasks (long and twisted necks). These flasks can exchange the air inside with the external air, but the microbes from the external air cannot enter into the flask, instead they trapped in the flask of the neck. He sterilized the broth in one of the flasks. With this experiment, he observed that the growth did not occur in the sterile broth.
After that he broke the necks of the flasks and allowed contamination of the broth, microbial growth was observed. Regarding this invention, Pasteur ascertained that "Omne vivum ex vivo" (Life only comes from life).
Nester's Microbiology 8th Edition by Denise Anderson,Sarah Salm,Deborah Allen
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