iGenetics 3rd Edition by Peter Russell

Exercise 6

You have inserted human insulin cDNA in the cloning vector pBluescript II (described in Figure, p. 176) and transformed the clone into E. coli , but insulin was not expressed. Propose several hypotheses to explain why not.
Figure
The plasmid cloning vector pBluescript II. This plasmid cloning vector has an origin of replication (ori), an amp R selectable marker, and a multiple cloning site located within part of the
galactosidase gene lacZ +.