Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 6

The Equilibrium Constant. The following reaction is one of the steps in the glycolytic pathway, which we will encounter again in Chapter 9. You should recognize it already, however, because we used it as an example earlier (Reaction 5-9):
(1)
The equilibrium constant K eq for this reaction at 25°C is 0.5.
(a)Assume that you incubate a solution containing 0.15 M glucose-6-phosphate (G6P)overnight at 25°C with the enzyme phosphoglucoisomerase that catalyzes Reaction 5-28. How many millimoles of fructose-6-phosphate (F6P)will you recover from 10 mL of the incubation mixture the next morning, assuming you have an appropriate chromatographic procedure to separate F6P from G6P
(b)What answer would you get for part a if you had started with a solution containing 0.15 M F6P instead
(c)What answer would you expect for part a if you had started with a solution containing 0.15 M G6P but forgot to add phosphoglucoisomerase to the incubation mixture
(d)Would you be able to answer the question in part a if you had used 15°C as your incubation temperature instead of 25°C Why or why not