Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 5

Michaelis-Menten Kinetics. Figure represents a Michaelis-Menten plot for a typical enzyme, with initial reaction velocity plotted as a function of substrate concentration. Three regions of the curve are identified by the letters A, B, and C. For each of the statements that follow, indicate with a single letter which one of the three regions of the curve fits the statement best. A given letter can be used more than once.
(a)The active site of an enzyme molecule is occupied by substrate most of the time.
(b)The active site of an enzyme molecule is free most of the time.
(c)This is the range of substrate concentration in which most enzymes usually function in normal cells.
(d)This includes the point ( K m , V max /2).
(e)Reaction velocity is limited mainly by the number of enzyme molecules present.
(f)Reaction velocity is limited mainly by the number of substrate molecules present.
Figure Analysis of the Michaelis-Menten Plot.