Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 13

QUANTITATIVE Monkeys and Peanuts Revisited. Recall the "enzyme-catalyzed reaction" of monkeys shelling peanuts earlier in the chapter. Here we will further increase the peanut concentration and determine the effect on the kinetic parameters of this animal "enzyme."
(a)Let's again triple the concentration of peanuts to 9 peanuts per square meter. Add another column to the following table and determine the overall rate of shelling for the ten monkeys.
(b)How did this second tripling of [S] affect the overall rate How does this compare to the effect of the first tripling
(c)Now increase [S] another tenfold to 90 peanuts per square meter. Add another column to the table and determine the overall rate of shelling for the ten monkeys.
(d)How did this tenfold increase in [S] affect the overall rate compared to the effect of the previous increases
(e)Graph the results as v on the y -axis (suggested scale: 0-10 peanuts/sec)versus [S] on the x -axis (suggested scale: 0-100 peanuts/m 2 ). What do you predict will be the V max Can you explain this logically What is the K m
(f)What is the K m for this shelling reaction