Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 2

The History of Glycolysis. Following are several historical observations that led to the elucidation of the glycolytic pathway. In each case, suggest a metabolic basis for the observed effect, and explain the significance of the observation for the elucidation of the pathway.
(a)Alcoholic fermentation in yeast extracts requires a heat-labile fraction originally called zymase and a heat-stable fraction (cozymase)that is necessary for the activity of zymase.
(b)Alcoholic fermentation does not take place in the absence of inorganic phosphate.
(c)In the presence of iodoacetate, a known inhibitor of glycolysis, fermenting yeast extracts accumulate a doubly phosphorylated hexose.
(d)In the presence of fluoride ion, another known glycolytic inhibitor, fermenting yeast extracts accumulate two phosphorylated three-carbon acids.