Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 4

DNA Melting. Figure shows the melting curves for two DNA samples that were thermally denatured under the same conditions.
(a)What conclusion can you draw concerning the base compositions of the two samples Explain.
(b)How might you explain the steeper slope of the melting curve for sample A
(c)Formamide and urea are agents known to form hydrogen bonds with pyrimidines and purines. What effect, if any, would the inclusion of a small amount of formamide or urea in the incubation mixture have on the melting curves
FIGURE Thermal Denaturation of Two DNA Samples.