Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 5

DNA Renaturation. You are given two samples of DNA, each oi which melts at 92°C during thermal denaturation. After denaturing the DNA, you mix the two samples together and then cool the mixture to allow the DNA strands to reassociate. When the newly reassociated DNA is denatured a second time, the sample now melts at. 85°C.
(a)How might you explain the lowering of the melting temperature from 92°C to85°C
(b)If the newly reassociated DNA had melted at 92°C instead of 85°C, what conclusions might you have drawn concerning the base sequences of the two initial DNA samples