Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 9

Going Nuclear. Experiments have been performed with the nuclear localization sequence (NLS)of nucleoplasmin. In each case, a string of amino acids was added to pyruvate kinase, a glycolytic enzyme normally found in the cytosol. The sequence of each added string of amino acids is shown using single-letter amino acid designations. Consult the single-letter amino acid code table (Table 3-2, page 45)if you feel it is necessary. Many alanines in a row are denoted by (A)n , where n is the number of alanines. The following results were obtained:
(a)What sort of NLS does nucleoplasmin appear to have, based on these results Explain your answer.
(b)How does your conclusion about the NLS of nucleoplasmin compare to the NLS for SV40 large T antigen