Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 12

Cloning Conundrum. Using established recombinant DNA technology, you insert a gene from a human liver cell into a bacterium. The bacterium then expresses a protein corresponding to the inserted DNA. To your dismay, you discover that the protein produced is useless and is found to contain many more amino acids than does the protein made by the eukaryotic cell. Assuming there is no mutation in the human gene, explain why this happened.