Becker's World of the Cell 9th Edition by Lewis Kleinsmith, Jeff Hardin, Gregory Paul Bertoni

Exercise 6

DNA Sequencing. You have isolated the DNA fragment shown below (which you actually analyzed in Problem 16-3, on page 464):
3 AGCGCTATAGCGCT5
5 TCGCGATATCGCGA3
As a training exercise for a young labmate, you provide her with this DNA and instruct her to perform a restriction digest. From knowledge of the specificity of the restriction enzyme used to produce the fragment, she knows the first four bases at the left end. She has prepared a single-stranded DNA primer of sequence 5 TCGC3 . You ask her to explain how she would determine the rest of the sequence using dye-labeled dideoxynucleotides, and you ask her to draw the gel pattern that would be observed, indicating the base sequence of the DNA in each band and the color pattern that would be detected by the camera in a DNA sequencing machine. What should her explanation contain, and what should her drawing look like
Problem 16-3
DNA Structure. Carefully inspect the double-stranded DNA molecule shown here, and notice that it has twofold rotational symmetry:
3 A-G-C-G-C-T-A-T-A-G-C-G-C-T 5
5 T-C-G-C-G-A-T-A-T-C-G-C-G-A 3
Label each of the following statements as T if true or F if false.
(a)There is no way to distinguish the right end of the double helix from the left end.
(b)If a solution of these molecules were heated to denature them, every single-stranded molecule in the solution would be capable of hybridizing with every other molecule.
(c)If the molecule were cut at its midpoint into two halves, it would be possible to distinguish the left half from the right half.
(d)If the two single strands were separated from each other, it would not be possible to distinguish one strand from the other.
(e)In a single strand from this molecule, it would be impossible to determine which is the 3 end and which is the 5 end.