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Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 10
Identify proteins using BLAST searches for peptide fragment sequences
Go to the National Center for Biotechnology Information web site at http://www.ncbi.nlm.nih.gov/. From the menu of Popular Resources on the right hand side, click on "BLAST". Under the Basic BLAST heading on the new page that comes up, click on "protein blast". In the Enter Query Sequence box at the top of the page that comes up enter the following sequence: NGMMKSSRNLTKDRCK. Confirm that the database under "Choose Search Set" is set on "nr" (nonredundant protein sequences), then click the BLAST button at the bottom of the page to see the results of your search. Next, enter this sequence from a different protein: SLQTASAPDVYAIGECA. Identify the protein from which this sequence was derived.
Go to the National Center for Biotechnology Information web site at http://www.ncbi.nlm.nih.gov/. From the menu of Popular Resources on the right hand side, click on "BLAST". Under the Basic BLAST heading on the new page that comes up, click on "protein blast". In the Enter Query Sequence box at the top of the page that comes up enter the following sequence: NGMMKSSRNLTKDRCK. Confirm that the database under "Choose Search Set" is set on "nr" (nonredundant protein sequences), then click the BLAST button at the bottom of the page to see the results of your search. Next, enter this sequence from a different protein: SLQTASAPDVYAIGECA. Identify the protein from which this sequence was derived.
Explanation
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A protein BLAST search for the protein s...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
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